<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP09405

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMASSSAGLEPGPSSSTDGANANLPIQASSSAAPSVIKGGNPQLSDEPWFAYPTPTPEQIEDELPPYFEGENIPLGLLLDRLVRKGYGDLRYLLAEVLPPLSAKHKPKHIINYATATRQALLKYLAVLRWKNAVDIPSTYHPSSSSSGTTGQQGNNNNNNSNNNNQNGTTNFPTPHSNGDDTSPPSILSNNKGKGKAYSAGGEGPGEEVTVKGKVSDSRRLAQFMEHQNKQHDDAVIHLKHVTKLVESLRERNPDLLTALSLLTTGTYQHLSTIITEPFIPKPPSTNSFILSLLKRLNRHIKYRLRCVDYLPPDLVVEGISDGQVYLRGGGKNGWKARMTIVGFGEDSKWWLTGVEWCWRDTRKGVYDPGGDSEEGGEGKKFVGEERQSILDLANLEILPPREVDIQRFYQQEEKGIEVEPQTVNIAAPDESTTTKSMDVELKDQKPKNTVVDAPLVRIYNFLQHLSLSYQLEILFSQAMTLSQGKWRNQLRVEIDRENKTLKVKYWVRPRPAVIPQQPQQAAVGKRPAPTANLNAARTPLVGGVLSITLGENGPRPTETDSILGDVSSGGLIPNERILRLQLGVKWEVGELGVGGGLKLGDVMDATALNVDPASLDMENLLMSSARAHAAHLTRVQASSLISSPKFILSLLNSPSLQESEDQTSSRPLTLRIPLPSRQRISSLLIGVSAYNGYIEIEDDGSIGDEARANRVELATKSINDGKTRLVDDIGRLTVAVVMENLEDQMRQSGWKPIRRLALRSQDLAKVQLHPATTILIPLPSTSSHYFVTKVTQGGLAFELLKTIKVPIENGLGVGTKAAVGDRIPLDLEKLKARRQDKDEMTASPFEIGNKDLRELYILSNALVAQTLIEQQLKERNIPFNAQYPPTSGPGAPRSSSALAGMVPTICVDVGDLLRDGKTGAAADVAMPKVGMQIEDWWKGGRCEVTTIVQLRQQSSMSQTSTPTTSQPHSEDISFDPASSIVKFRAKDIERCVPAFLEQWERLSKVIVVAGEVNRLNKLAEFRDIKMLSFDLRTATLSYSKGYNASITYIPTNDSYQVILSTDHTSTSPAFETDSPSPVQKERHNPHEKLSQLLSARLTELASQPLESRGSIGKEFFGLLKNTLPFFEQVDKLEKRNWNLLIMDLRNWRIVRDHESRRYALDIILIPSLTHYLIQSSSKPRGPDQRIVDITGKLNELPLTKIIPKVFDNFKEQQHTPSGGKREIPPLMKLDKNKSMLCKVDLIESVLEGFLNEVDLVIGVKKE
Length1262
PositionTail
OrganismKwoniella mangroviensis CBS 10435
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Agaricomycotina> Tremellomycetes> Tremellales> Cryptococcaceae> Kwoniella.
Aromaticity0.06
Grand average of hydropathy-0.424
Instability index45.89
Isoelectric point7.34
Molecular weight139438.84
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP09405
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     771.29|     201|     218|     488|     703|       1
---------------------------------------------------------------------------
  488-  703 (302.69/226.59)	NqlRVEID....RENKT............LKVKYWV...RPRPAVIPQ..QPQQAAVGKRPAPTANL...NAARTPLvGGVLSITLgengPRP.TETDSILGDVSSGGLIPNERILRL..QLGVKWE.VGELGVGGGL.....KLGDVMDATALnvdpaSLDMENL..........LMSSARAHAAHLTRVQASsliSSPKFILS.LLNSPSLQESEDQTSSRPLTLRIPL...PSRQRISSLLIGVSAYNGYIEIEDDGSIG
  709-  911 (233.80/146.03)	N..RVELAtksiNDGKT............RLVDDIG...RLTVAVVMEnlEDQMRQSGWKPIRRLALrsqDLAKVQL.HPATTILI....PLPsTSSHYFVTKVTQGGL.AFE.LLK...TIKVPIE.NG.LGVGTKA.....AVGDRI..........PLDLEKLkarrqdkdemTASPFEIGNKDLREL..........YILSnALVAQTLIEQQLKERNIPFNAQYPPtsgPGAPRSSSALAGMVP.TICVDVGD.....
  914- 1112 (234.80/146.16)	............RDGKTgaaadvampkvgMQIEDWWkggRCEVTTIVQ..LRQQSSMSQTSTPTT.........................SQP.HSEDISFDPASSIVKFRAKDIERCvpAFLEQWErLSKVIVVAGEvnrlnKLAEFRDIKML.....SFDLRTA..........TLSYSKGYNASITYIPTN...DSYQVILS..TDHTSTSPAFETDSPSPVQKERHN...P.HEKLSQLLSARLTELASQPLESRGSIG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.55|      14|     174|     194|     207|       2
---------------------------------------------------------------------------
  194-  207 (27.61/16.41)	GKAYSAGGEGP...GEE
  369-  385 (21.93/11.39)	GGDSEEGGEGKkfvGEE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.33|      18|    1039|     173|     191|       6
---------------------------------------------------------------------------
  173-  191 (30.05/21.43)	TPhSNGDDTSPPSI.LSNNK
 1215- 1233 (30.27/16.12)	TP.SGGKREIPPLMkLDKNK
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP09405 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) PSTYHPSSSSSGTTGQQGNNNNNNSNNNNQNGTTNFPTPHSNGDDTSPPSILSNNKGKGKAYSAGGEGPGEEVTVKGKVSDSRRLAQFMEHQNKQHDDAVIHLKHVTK
136
243










Molecular Recognition Features






MoRF SequenceStartStop




1) DEPWFAYPTP
2) IEDELP
45
59
54
64