<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP08224

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAPQPLEQGGAVTSFIPTGQEMAQRQNLIPLGRLIDFIIQRTYHELTVLAELLPRKTDMDRKIEIYNFSASTRQLFIRLLALVKWANSASKVDKSAKIMGFLDKQSMLFIDTADMLSRVARETLVHARLPNFHIPAAVEILTTGSYSRLPSVIRDRIVPPDPITPAEKRQTLQRLNQVIQHRLVTGSLLPQLRKFRIENGRVTFKVDHEFEVSLTVMGDAPTVPWRLLDIDFLVEDKETGDGKALVHPLQVNYIHQLIQGRIVDCTDALAEVYTCLHYFCQSLQLEVLYTQTLRLIRDRLDDHIHVDEYVVGSRLTVSYWRELTNKDPKSELGYRLTIQTDPNDAAKQLAILHVPSIGNKEADIADRAVRSDLLSMERLLVHTVYVRSLARLNDVKTELQLFLKDVEYHIQGTPAMLTVPVLNPCLRAEHIYITVDTHTGMLRCHVPKHLDCPIMPEMQHALNNDWSKLQHLISELRYWITQRRCEKTLQHLPAATQDRLPLIYPQNHPIARMGPHKVFIQLYRHANVILIVELKEKKSCPNEMTYTFYLVLVKPSSVEEGQSQDAAAAAAGQANQAGGNETATAAMPKMYLRVLSMIEFDTFVATHGPGTYIDDPTPTSSGSTSVSTKRKLSPLESAISSMGPPLKQQKTIYPAYFIPELAHVVAMCDEKLPFVTLAKEFTMRKIPHGGLQVEANATSLVLKLLTLPQPKPPQAPTTAQQQQQQQQSGASGADAKAAAAAAASANEPKTVHVPPIDKQVWNALLKRLLSVSVRAQVNKSNQTRLWTMELVFYGSPLPSLHHKEQGMRRAVYLQYEMQPVESVSKVVDQLLSDWSKIVYLYTLVHEFREQFNTEKYNLQSMVTIKSYSYTNLLLAYGPNKDVSVNICWDTEAKEFRLVFTGGNSAINAHSMMRDQLQAHLNHNYSLAQIVHMLHETYQPLSSIAKLPIIPHLAILQSPKIPVLSFCIIPQSPTLLRISFQGVYCLEVRFRGGGLCTIRDGAYSRFDRSHVVEEFTPTQGLKGFLSKYVDETAVFRRRSQSEDDNPPSPVTLEDPSGHGGGAGGSSGGGNNGGAGGGGGTGGGTGTFLSGGTGMRGPQSPRDPGLRFAAPLTPPTGSNPHTPASPHPIGGGGGGGGAGGAGGGGTGGAGGQGGQQQGHMNNFNMTSPPASHMPHPSPSGGLMPSSPLNAQPSPMAAHSPGPSSLPYMQSHTEGSPFAALSPAASNWPGSPGMPRPSPRPGQSPEHKVQTSHHYASRVLPARSWAGATPTTLTYEALDTLCRATSHPQKEVPGPELSPLERFLGSVFMRRQLQRIIHQEESLMAITSNEPGVVVFKADCLQYQVFLNPNHMQSLHLKVDQLPMGPMMDGKPPYQWAAQDLQILEQFFDQRVAAPPYRPAVMTSFTRMLNLPAKVLKDFIQIMRLDLMPELVQGNKWNVQFVLRVPPSATPIVPVGTTTILSHRQKILFFIQITRVPYMPNMEWKDAVTMLLPMVYDMNMNHTTLAERREPMPPQLNSAVSAHLRRFSECSVLLPDECSLFPAVHDLLLSLTLPNEPPAPGQMQMQMGGVMQPGGGPGGPGGPGGPMGGQIGGPSPQVVPQVGSSPSPMMHSPMQQMGGGGVQQPGGYGGMVGGPGGGPQSGGPGGGGPGGPN
Length1650
PositionTail
OrganismAnopheles stephensi (Indo-Pakistan malaria mosquito)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Nematocera> Culicoidea> Culicidae> Anophelinae> Anopheles.
Aromaticity0.07
Grand average of hydropathy-0.269
Instability index52.78
Isoelectric point8.55
Molecular weight180452.96
Publications
PubMed=25244985

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP08224
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     440.09|      64|      64|    1063|    1126|       1
---------------------------------------------------------------------------
 1036- 1092 (73.67/21.51)	.RRSQSEDD...............NPPSP..VtledpSGHG..ggagGSS...G..G....GNNGGAGGGG.GT..GGGTGTFLSGGTG
 1093- 1159 (121.10/39.65)	MRGPQSPRDPGLRFAAPLTPPTGSNPHTP..A.....SPHP....igGGG...G..G....GGAGGAGGGGtGG..AGGQGGQQQGHMN
 1160- 1230 (89.53/27.58)	NFNMTSP..PASHMPHP.SPSGGLMPSSPlnA.....QPSPmaahspGPS...SlpY....MQSHTEGSPF.AA..LSPAASNWPGSPG
 1548- 1592 (66.14/18.63)	.................LTLP..NEPPAP..G.....QMQM.......QM...G..GvmqpG..GGPGGPG.GP..GGPMGGQI.GGPS
 1593- 1650 (89.65/27.62)	...PQVVPQVG.....S.SPS..PMMHSP..M.....QQMG......GGGvqqP..G....GYGGMVGGPG.GGpqSGGPGGGGPGGPN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     229.42|      70|     314|     163|     234|       2
---------------------------------------------------------------------------
  163-  234 (113.75/92.41)	ITPAEKRQTLQRLNQVIQHRLVTgsLLPQLRKF.RIENGRVTFKVDHEFEVSLTV.MGDAPTVPWRLLDIDFLV
  480-  551 (115.67/86.81)	ITQRRCEKTLQHLPAATQDRLPL..IYPQNHPIaRMGPHKVFIQLYRHANVILIVeLKEKKSCPNEMTYTFYLV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     125.64|      46|     218|    1255|    1306|       3
---------------------------------------------------------------------------
 1255- 1301 (78.81/60.64)	RVL.PARSWAGAT...PTTLTYEAlDTL....CRATSHPQ......KEVP.GP.............ELSPLERFL
 1312- 1385 (46.82/21.05)	RIIhQEESLMAITsnePGVVVFKA.DCLqyqvFLNPNHMQslhlkvDQLPmGPmmdgkppyqwaaqDLQILEQFF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     131.84|      38|      49|    1392|    1434|       4
---------------------------------------------------------------------------
 1392- 1434 (62.41/59.15)	PP.YRPAVMTSFTRMLNLPAKVLKdFIQIMRLDLMPELvqgnKW
 1443- 1481 (69.43/48.79)	PPsATPIVPVGTTTILSHRQKILF.FIQITRVPYMPNM....EW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.67|      21|     523|     708|     728|       8
---------------------------------------------------------------------------
  708-  728 (41.34/23.86)	PQPKPPQAPTTAQQQQQQQQS
 1234- 1254 (41.33/23.86)	PSPRPGQSPEHKVQTSHHYAS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     154.66|      49|     549|     288|     341|       9
---------------------------------------------------------------------------
  288-  341 (74.17/62.37)	LYTqtlrLIRDrLDDHIHVDEYVVGSRLTV...SYWRELTNKDPKSELGYRLTIQTD
  840-  891 (80.49/53.00)	LYT....LVHE.FREQFNTEKYNLQSMVTIksySYTNLLLAYGPNKDVSVNICWDTE
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP08224 with Med14 domain of Kingdom Metazoa
Unable to open file!