<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP08218

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAPQPLEQGGAVTSFIPTGQEMAQRQNLIPLGRLIDFIIQRTYHELTVLAELLPRKTDMDRKIEIYNFSASTRQLFIRLLALVKWANSASKVDKSAKIMGFLDKQSMLFIDTADMLSRVARETLVHARLPNFHIPAAVEILTTGSYSRLPSVIRDRIVPPDPITPAEKRQTLQRLNQVIQHRLVTGSLLPQLRKFRIENGRVTFKVDHEFEVSLTVMGDAPTVPWRLLDIDFLVEDKETGDGKALVHPLQVNYIHQLIQGRIVDCTDALAEVYTCLHYFCQSLQLEVLYTQTLRLIRDRLDDHIHVDEYVVGSRLTVSYWRELTNKDPKSELGYRLTIQTDPNDAAKQLAILHVPSIGNKEADIADRAVRSDLLSMERLLVHTVYVRSLARLNDVKTELQLFLKDVEYNIQGTPAMLTVPVLNPCLRAEHIYITVDTHTGMLRCHVPKHLDCPIMPEMQHALNNDWSKLQHLISELRYWITQRRCEKTLQHLPAATQDRLPLIYSHTHPIARMGPHKVFIQLYRHANVILIVELKEKKTCPNEMTYTFYLVLVKPSSVEEGQSPDVLSGQPPQPSAAGGAAPGSDAANAAMPKMYLRVLSMIEFDTFVATHGPGTYIDDPCPTSTSGTSVKRKVSPLDNALSAIGPPLKQQKTIYPAYFIPELAHVVAMCDEKLPFVTLAKEFSMRKIPHGGLQVEANATSLVLKLLTLPQPKPPQAPPTPQQQQQQQQQQQPGTSDAKSSGAGASANEPKTVHVPPIDKQVWNALLKRLLSVSVRAQVNKSNQTRLWTMELVFYGSPLPSLHHKEQGMRRAVYLQYEMQPVESVSKVVDQLLSDWSKIVYLYTLVHEFREQYNNEKYNLPSMVAIKSYSYTNLLLAYGPNKDVSVNICWDTEAKEFRLVFTGGNSAINAHSMMRDQLQAHLNHNYSLAQVVHMLHETYQPLSSIAKLPIIPHLAILQSPKIPVLSFCIIPQSPTLLRISFQGVYCLEVRFRGGGLCTIRDGAYSRFDRSHVVEEFTPTQGLKGFLSKYVDETAVFRRRSQSEDDNPPSPVTLEDPSAGAGNNGGGGGGGAGAGGGANTFLSGGTGMRGPQSPRDPGLRFAAPLTPPTSSNPHTPASPHPIGGGGGAGGAGGQGGQGGQGGQQQGHMNNFNMTSPPASHMPHPSPGGGLMPSSPLNAQPSPMAAHSPGPSSLSYMQSHTDGSPFAALSPAASNWPGSPGMPRPSPRPGQSPEHKVQTSHHYTSRVLPARSWAGAIPTTLTYEALDTLCRATPHPQKEVPGPELSPLERFLGSVFMRRQLQRIIHQEESLMAITSNEPGVVVFKADCLQYQVFLNPNHMQSLHLKVDQLPMGPMMDGKPPYQWAAQDLQILEQFFDHRVAAPPYRPAVMTSFTRMLNLPAKVLKDFIQIMRLDLMPELVQGNKWNVQFVLRVPPSATPIVPVGTTTILSHRQKILFFIQITRVPYLPNMEWKDAVTMLLPMVYDMNMNHTTLAERREPMPPQLTSAVSAHLRRFSECSVLLPDECSLFPAVHDLLLTLTLPNEPPAPGQMQMQQLGGVMQPGGGPGVPGGPGGPMGGQIGGPTPQVVPQVGSSPSPMMHSPMQQMGGGGPQPGAYGGMVGGPGGGPQSGGPVGGGPGGPN
Length1639
PositionTail
OrganismAnopheles quadriannulatus (Mosquito)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Nematocera> Culicoidea> Culicidae> Anophelinae> Anopheles.
Aromaticity0.07
Grand average of hydropathy-0.273
Instability index51.73
Isoelectric point8.53
Molecular weight180083.85
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP08218
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     345.60|      42|      49|    1540|    1581|       1
---------------------------------------------------------------------------
 1045- 1077 (49.79/14.29)	.DNPP....SP..V...TLE..........DPS......AGAGNNGGG..GGGGAGAGGGA
 1112- 1142 (62.55/19.76)	PHTPA....SP........HPI.G...G....G......GGAGGAGGQ..GGQ.GGQ.GGQ
 1153- 1179 (47.34/13.24)	TS.PP....A.SHM...PHPS..........PG......GG....LMP..SSPLNAQ...P
 1187- 1219 (33.37/ 7.24)	PG.PS....SLSYM...QSHTD.GspfAALSPA......AS.NWPGSP..G..........
 1540- 1581 (91.49/32.18)	PNEPP....APGQM...QMQQL.G...GVMQPG......GGPGVPGGP..GGPMGGQIGGP
 1583- 1638 (61.06/19.12)	PQVVPqvgsSPSPMmhsPMQQMgG...GGPQPGayggmvGGPG..GGPqsGGPVGGGPGGP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     112.97|      37|     130|     174|     303|       2
---------------------------------------------------------------------------
  245-  293 (50.28/177.42)	LVHPLqvnYIHQLiqGRivdctdaLAEVYTCLHYFCQSLQLEVLYTQTL
  380-  416 (62.69/ 8.56)	LVHTV...YVRSL..AR.......LNDVKTELQLFLKDVEYNIQGTPAM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.66|      13|      41|     711|     723|       3
---------------------------------------------------------------------------
  711-  723 (27.07/12.09)	QPKPPQAPPTPQQ
  749-  761 (24.59/10.25)	EPKTVHVPPIDKQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|      89.26|      16|      41|     141|     157|       4
---------------------------------------------------------------------------
  142-  157 (27.33/17.12)	TTGSYSRLPSVIRDRI
  168-  183 (26.89/11.45)	KRQTLQRLNQVIQHRL
  185-  197 (17.85/ 6.97)	.TGSL..LPQLRKFRI
  488-  500 (17.18/ 6.37)	...TLQHLPAATQDRL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     162.20|      62|     566|     915|     987|       6
---------------------------------------------------------------------------
  915-  984 (100.36/70.92)	R.DQLQAHLNHNYSLAQVVHMLHEtYQP...........................................................................................................................................................................................................................................................................................................................................................................................................................lssiaklPIIP.HLAILQSPKIPVLSFCIIPQSPTLLRISFQG........V
  992- 1018 (23.96/ 7.31)	RgGGLCTIRDGAYSRFDRSHVVEE.FTP...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................
 1437- 1481 (37.88/14.18)	............................tqglkgflskyvdetavfrrrsqseddnppspvtledpsagagnngggggggagagggantflsggtgmrgpqsprdpglrfaapltpptssnphtpasphpigggggaggaggqggqggqggqqqghmnnfnmtsppashmphpspggglmpssplnaqpspmaahspgpsslsymqshtdgspfaalspaasnwpgspgmprpsprpgqspehkvqtshhytsrvlparswagaipttltyealdtlcratphpqkevpgpelsplerflgsvfmrrqlqriihqeeslmaitsnepgvvvfkadclqyqvflnpnhmqslhlkvdqlpmgpmmdgkppyqwaaqdlqileqffdhrvaappyrpavmtsftrmlnlpakvlkdfiqimrldlmpelvqgnkwnvqfvlrvppsatPIVPvGTTTILSHRQKILFFIQITRVPYLPNMEWKDavtmllpmV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      57.05|      17|      21|    1343|    1363|       7
---------------------------------------------------------------------------
 1343- 1363 (30.14/28.95)	LKVdqlpMGPMMDGK...PPYQWA
 1367- 1386 (26.90/14.26)	LQI....LEQFFDHRvaaPPYRPA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      34.23|       9|     130|    1087|    1097|      10
---------------------------------------------------------------------------
 1087- 1097 (12.46/11.18)	MRGPqSPRdPG
 1220- 1228 (21.77/ 8.70)	MPRP.SPR.PG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     124.86|      41|     315|     502|     566|      12
---------------------------------------------------------------------------
  505-  555 (54.49/59.78)	SHTHPIARmgPHKV...FIQLYRhanVILIVELKEkktcPNEMTYTFYLvLVKP
 1390- 1433 (70.37/36.69)	SFTRMLNL..PAKVlkdFIQIMR...LDLMPELVQ....GNKWNVQFVL.RVPP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP08218 with Med14 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FRRRSQSEDDNPPSPVTLEDPSAGAGNNGGGGGGGAGAGGGANTFLSGGTGMRGPQSPRDPGLRFAAPLTPPTSSNPHTPASPHPIGGGGGAGGAGGQGGQGGQGGQQQGHMNNFNMTSPPASHMPHPSPGGGLMPSSPLNAQPSPMAAHSPGPSSLSYMQSHTDGSPFAALSPAASNWPGSPGMPRPSPRPGQSPEHKVQTSHHYTS
2) LLTLPQPKPPQAPPTPQQQQQQQQQQQPGTSDAKSSGAGASANEPKTVH
3) LTLPNEPPAPGQMQMQQLGGVMQPGGGPGVPGGPGGPMGGQIGGPTPQVVPQVGSSPSPMMHSPMQQMGGGGPQPGAYGGMVGGPGGGPQSGGPVGGGPGGPN
1036
706
1537
1243
754
1639










Molecular Recognition Features






MoRF SequenceStartStop




NANANA