<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP08068

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAPQPLEQGGAVTSFIPTGQEMAQRQNLIPLGRLIDFIIQRTYHELTVLAELLPRKTDMDRKIEIYNFSASTRQLFIRLLALVKWANSASKVDKSAKIMGFLDKQSMLFIDTADMLSRVARETLVHARLPNFHIPAAVEILTTGSYSRLPSVIRDRIVPPDPITPAEKRQTLQRLNQVIQHRLVTGSLLPQLRKFRIENGRVTFKVDHEFEVSLTVMGDAPTVPWRLLDIDFLVEDKETGDGKALVHPLQVNYIHQLIQGRIVDCTDALAEVYTCLHYFCQSLQLEVLYTQTLRLIRDRLDDHIHVDEYVVGSRLTVSYWRELTNKDPKSELGYRLTIQTDPNDAAKQLAILHVPSIGNKEADIADRAVRSDLLSMERLLVHTVYVRSLARLNDVKTELQLFLKDVEYHIQGTPAMLTVPVLNPCLRAEHIYITVDTHTGMLRCHVPKHLDCPIMPEMQHALNNDWSKLQHLISELRYWITQRRCEKTLQHLPAATQDRLPLIYPQNHPIARMGPHKVFIQLYRHANVILIVELKEKKTCPNEMTYTFYLVLVKPSSVEEGQSQDTASAGQTNAPSGSGGHPSSNETATAAMPKMYLRVLSMIEFDTFVATHGPGTYIDDPSTTCTSTKRKISPLDSALSSMGPPLKQQKTIYPAYFIPELAHVVAMCDEKLPFVTLAKEFTMRKIPHGGLQVEANATSLVLKLLTLPQPKPPQVPPSAQQQQQQQQQQQQQQSGQSGSDAKAAAAAAANEPKTVHVPPIDKQVWNALLKRLLSVSVRAQVNKSNQTRLWTMELVFYGSPLPSLHHKEQGMRRAVYLQYEMQPVESVSKVVDQLLSDWSKIVYLYTLVHEFREQFNNEKYNLQSMVTIKSYSYTNLLLAYGPNKDVSVNICWDTEAKEFRLVFTGGNSAINAHSMMRDQLQAHLNHNYSLAQIVHMLHETYQPLSSIAKLPIIPHLAILQSPKIPVLSFCIIPQSPTLLRISFQGVYCLEVRFRGGGLCTIRDGAYSRFDRSHVVEEFTPTQGLKGFLSKYVDETAVFRRRSQSEDDNPPSPVTLEDPSGHGGAGGGTGGGNNGGAGGGGGTGGGSNTFLSGGAGMRGPQSPRDPGLRFAAPLTPPTSSNPHTPASPHPIGGGGGAGGAGGGGAGGTGGQGGQQQGHMSNFNMTSPPASHMPHPSPSGGLMPSSPLNAQPSPMAAHSPGPSSLPYMQSHTDGSPFAALSPAASNWPGSPGMPRPSPRPGQSPEHKVQTSHHYTSRVLPARSWAGAIPTTLTYEALDTLCRATSHPQKEVPGPELSPLERFLGSVFMRRQLQRIIHQEESLMAITSNEPGVVVFKADCLQYQVFLNPNHMQSLHLKVDQLPMGPMMDGKPPYQWAAQDLQILEQFFDQRVAAPPYRPAVMTSFTRMLNLPAKVLKDFIQIMRLDLMPELVQGNKWNVQFVLRVPPSATPIVPVGTTTILSHRQKILFFIQITRVPYMPNMEWKDAVTMLLPMVYDMNMNHTTLAERREPMPPQLNSAVSAHLRRFSECSVLLPDECSLFPAVHDLLLTLTLPNEPPAPGQMQMQMGGVMQPGGGPGGPGGPGGPMGGQIGGPSPQVVPQVGSSPGPMMHSPMQQMGGGGQQPGGYGGMVGGPGGGPQSGGPGGGGPGGPN
Length1649
PositionTail
OrganismAnopheles funestus (African malaria mosquito)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Nematocera> Culicoidea> Culicidae> Anophelinae> Anopheles.
Aromaticity0.07
Grand average of hydropathy-0.296
Instability index53.84
Isoelectric point8.54
Molecular weight180975.50
Publications
PubMed=21129198
PubMed=31157884

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats
>MDP08068
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     360.43|      60|      62|    1069|    1130|       1
---------------------------------------------------------------------------
 1069- 1105 (65.12/20.67)	.............................GGGNN..GGAGG.GG........GTGG.G.......SNTFLSGGAGMRGPQSPRDP
 1106- 1172 (81.46/31.65)	GLRFAAPLTPPTSsnPHTPASPHPI...gGGGGA..GGAGG.GG......agGTGGqGgqqqghmSN.F.....NMTSPPASHMP
 1179- 1229 (67.73/22.13)	GLMPSSPLNAQPS..PMAAHSPGP....................sslpymqsHTDG.S.......PFAALSPAAS....NWPGSP
 1551- 1603 (70.17/22.84)	...............PNEPPAPGQMqmqmGGGGP..GGPGG.PG........GPMG.G.......Q...IGGPSPQVVPQVGSSP
 1604- 1648 (75.95/25.68)	GPMMHSPMQQ..............M....GGGGQqpGGYGGmVG........GPGG.G.......PQ...SGGPGGGGPGGP...
---------------------------------------------------------------------------
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     281.95|      88|     480|     435|     580|       2
---------------------------------------------------------------------------
  163-  252 (142.56/76.25)	ITPAEKRQTLQRLNQVIQHRLVTgsLLPQLRKF.RIENGRVTFKVDHEFEVSLTV.MGDAPTVPWRLLDIDFLVEDK....ETGDGKALVHPLQVN
  480-  573 (139.39/142.36)	ITQRRCEKTLQHLPAATQDRLPL..IYPQNHPIaRMGPHKVFIQLYRHANVILIVeLKEKKTCPNEMTYTFYLVLVKpssvEEGQSQDTASAGQTN
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.53|      13|      41|     709|     721|       3
---------------------------------------------------------------------------
  709-  721 (26.44/11.56)	QPKPPQVPPSAQQ
  751-  763 (25.09/10.58)	EPKTVHVPPIDKQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.64|      19|      65|     314|     332|       4
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  314-  332 (35.64/27.69)	RLTVS..YWRELTN.KDPKSEL
  378-  399 (24.00/15.87)	RLLVHtvYVRSLARlNDVKTEL
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.92|      16|      17|    1474|    1490|       5
---------------------------------------------------------------------------
 1459- 1479 (20.53/ 8.13)	SHRQKILFFIqitrvPYMPNM
 1480- 1497 (19.39/10.06)	EWKDAVTMLL...pmVYDMNM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.00|      15|      17|    1238|    1252|       6
---------------------------------------------------------------------------
 1238- 1252 (28.14/16.36)	PGQSPEHKVQTSHHY
 1258- 1272 (25.86/14.39)	PARSWAGAIPTTLTY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      86.76|      27|      42|     801|     828|       7
---------------------------------------------------------------------------
  801-  828 (44.02/37.67)	LPSLHH..KEQGMRRAVYLQyEMQPVESVS
  844-  872 (42.74/30.19)	LYTLVHefREQFNNEKYNLQ.SMVTIKSYS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     125.80|      47|      65|    1309|    1373|      10
---------------------------------------------------------------------------
 1284- 1346 (64.95/46.54)	HPQ......KEVP.GPELS...PlerflgsvfMRR...QLQrIIHQ.EESLMAITSNEPGVVVfkadclQYQVFLN.P
 1348- 1409 (60.85/53.84)	HMQslhlkvDQLPmGPMMDgkpP.........YQWaaqDLQ.ILEQfFDQRVAAPPYRPAVMT......SFTRMLNlP
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP08068 with Med14 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FRRRSQSEDDNPPSPVTLEDPSGHGGAGGGTGGGNNGGAGGGGGTGGGSNTFLSGGAGMRGPQSPRDPGLRFAAPLTPPTSSNPHTPASPHPIGGGGGAGGAGGGGAGGTGGQGGQQQGHMSNFNMTSPPASHMPHPSPSGGLMPSSPLNAQPSPMAAHSPGPSSLPYMQSHTDGSPFAALSPAASNWPGSPGMPRPSPRPGQSPEHKVQTSHHYTS
2) LTLTLPNEPPAPGQMQMQMGGVMQPGGGPGGPGGPGGPMGGQIGGPSPQVVPQVGSSPGPMMHSPMQQMGGGGQQPGGYGGMVGGPGGGPQSGGPGGGGPGGPN
3) TLPQPKPPQVPPSAQQQQQQQQQQQQQQSGQSGSDAKAAAAAAANEPK
1038
1546
706
1254
1649
753










Molecular Recognition Features






MoRF SequenceStartStop




1) VGGPGGGPQSGGPGGGGP
1628
1645