<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP07727

Description RNA polymerase II mediator complex component Srb8
SequenceMISHSSSRRPPSRPPAPSRGSSSHSVVPYPASDSYPAIDSRAHQNEVIDLTESDGGGMADIGRPAKRPRLDVGNEPPQSSLDHTHNQHQQQQYQNQDQPSSQHHVSSMTGETPESSMNPLDLMSSSSTEQTGRPPWSFKGEIPRSDAEAQPEASQWPQSSSLPPLPIRPWKYGPQERYLAGTTDSKGGKECGAVKTTPYRIDVPSIAPRFADNKPADFSPWRGSHPEDVLSEQTAKQGFYDRASVSNETTSAKSVVYPQFKNRSGLKLLSSVFAAALEKRQALRTITAASTFRPPPRVTLTDIKREAWLRDLANPSVPLRRLSRTIPHGIRGKVLLDQCLGKSIPICRAIWLAKCVGANELRAFKRKGTSAGVASGLEAKWVRDWTISVQQFVEGVIAGIGDANWRLRISYSIRLLARLFLEHLVEQDHFLEWFLTSFHNSSVENLPVWLLMVGIYWENIVRYRKRGKRLAECLLEKLRLATEADQHAHLNPLTRRLSRLTKTLSLSHPSCFILPRCWDKYENVLSSCLDMNLSDDKAGFQNLKARNARVCRLGNSRRKPTQSTNQRLIQLLDSSSDVSTIGTECLNISQDYSTLATKVIEWASTSFRYGLARIYTAVRVFRRWKKTGIDIDSHLLSFLVQDHRKSGVRFLDVYHVICELVRSQSFSVAKYLQWLMARGSVSNVLGCPQMHIPGDIGLLGHLPSSRLPNHIWNLRNTLLSRTGFLVALEVDTIQRIKASVRRRLPNMFPKAPPEGDAEMTDDVDFSSLSWTVRSEIGNWIRDQVASHVKDSLKSAMDRDFGSEVKTSALIPQEFFEVRYILECLCDLSMLADVLKYASGSDNVTVLASAVDTLNCHLDSFNAIGATSDLFKSFTSAYARISKAELSVQDLIASLLDVAIKLPGELSTVSLLRRDLLQRDRKLAMAACSPVSDHIVDTLNTSNPTFTEELDQLLTSGNSMDESTVARIFDSLCKRLEAGSVNGEQCAHDIARYFAQLRPFNAKLFDNLMIKWVISVLRSSPRPNLSTMLPPLIGVGCVTLHSFYVLARALLHSDVHKSTIPDLAELRISMIQLLDSRLSNGNGSQDLVAYRFKIARQEYMRQFSGEALGLIHDLLAELSEGGTDSLPKQFNSELLSTVVTPLLCEIIVRHPATISADYGIRLLEKFPACINLTDRALGHLLASQVQSGLVAAEETIESINDFSLPLCLMKLRLLFGFECDSNVKKRIYDVVFETAKSNMRKGQSHWIDVVGSLHADAAKEIRQRAEEQLLSHVLTSDSLPTSPTSESNTPPGFNVSALVCLRIVEDLSFSIPETGSPSLCPTLIEKMNMILHRITTLENNLMEANISQDNDHATAIRQAHVVQQSSVIAFWFSVLLRLIAIHRSRFAPGTLSKSDLADQTRLLISINCIALSRTLSPKAPACPRPFPSTSPLTLTTPLQEPRPHFPFHVTDTGISTSLQIQALDVAATLLDSIPDDYRHQCARFLRDRCPPFLHPQNDPRLLFLLGPLAADNQPSPTTTQSSQQQPGGSTLSQANPGTPGTPATPLVVASVGATAASQSSQQFQGGNSYNAATFNPFDDQNSIVGKLRVQQRGRIAGPYPLRPWEMLEESAPVIGVNDTAVNLGWFAARRIRGDVA
Length1635
PositionKinase
OrganismBlastomyces gilchristii (strain SLH14081) (Blastomyces dermatitidis)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Eurotiomycetidae> Onygenales> Ajellomycetaceae> Blastomyces.
Aromaticity0.07
Grand average of hydropathy-0.246
Instability index50.18
Isoelectric point8.57
Molecular weight181174.19
Publications
PubMed=26439490

Function

Annotated function Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. Component of the srb8-11 complex. The srb8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The srb8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. 
ECO:0000256	ARBA:ARBA00002895
ECO:0000256	ARBA:ARBA00003744
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP07727
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      97.79|      27|      35|     113|     146|       1
---------------------------------------------------------------------------
  113-  144 (43.05/37.36)	PESSMNPldlmSSSSTEQTGRPPWSFkGEIPR
  151-  177 (54.74/26.53)	PEASQWP....QSSSLPPLPIRPWKY.GPQER
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      97.71|      28|      35|    1506|    1533|       2
---------------------------------------------------------------------------
 1506- 1533 (50.18/30.77)	PLAADNQPSPTTTQSSQQQPGGSTLSQA
 1544- 1571 (47.53/28.71)	PLVVASVGATAASQSSQQFQGGNSYNAA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     467.66|     144|     176|     318|     470|       3
---------------------------------------------------------------------------
  318-  459 (204.63/160.60)	.................................PL.RRLSR...TIPHGIRGKVLLDQCLGKSIPIcraiwLAKCVGAN..ELRA.FKR.KGTSAGVASGLEAK.........wVRDWTISV.QQFVEGV.....IAGIGDANW....RLRISYSIRLLARLFLEHLveQDHFLEWFLTSFH......NSSVENLPVWlLMVGIYWEN
  460-  633 (180.69/116.39)	IVR.YRKRGKRLaeclleklrlateadqhahlnPLtRRLSRltkTLSLSHPSCFILPRCWDKYENV.....LSSCLDMNlsDDKAgFQNlKARNARVCRLGNSR...........RKPTQSTnQRLIQLLdsssdVSTIGTEC......LNISQDYSTLAT..........KVIEWASTSFRyglariYTAVRVFRRW.KKTGIDIDS
  639-  717 (82.34/48.12)	LVQdHRKSGVRF............................................................................................ldvyhvicelVRSQSFSV.AKYLQWL.....MARGSVSNVlgcpQMHIPGDIGLLGHLPSSRL..PNHI..W...NLR......NT..................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     210.70|      75|     117|     832|     907|       4
---------------------------------------------------------------------------
  787-  867 (115.97/72.73)	HVKDSLKSAMDRDFGSEVKTSALIPQeFFEVRYILE.CLCD.lsmlaDVL....KYASG.....SDN.VTVLASAVDTLNCHLDSFNAIGATS
  868-  959 (94.73/62.62)	DLFKSFTSAYARISKAELSVQDLIAS.LLDVAIKLPgELSTvsllrrDLLqrdrKLAMAacspvSDHiVDTLNTSNPTFTEELDQLLTSGNSM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.52|      19|      37|    1067|    1086|       6
---------------------------------------------------------------------------
 1067- 1086 (27.94/23.82)	ISMIQLLDSRLSNGnGSQDL
 1107- 1125 (31.57/21.26)	LGLIHDLLAELSEG.GTDSL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      54.44|      17|      37|    1153|    1169|       8
---------------------------------------------------------------------------
 1153- 1169 (30.11/23.53)	ISADYGIRLLEKF..PACI
 1189- 1207 (24.32/17.26)	VAAEETIESINDFslPLCL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.73|      20|     105|    1299|    1320|      10
---------------------------------------------------------------------------
 1263- 1289 (24.27/ 9.13)	RAEEQLlshvltSDSLPTSpTSESNTP
 1301- 1320 (37.46/18.35)	RIVEDL......SFSIPET.GSPSLCP
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP07727 with Med12 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DNKPADFSPWRGSHPEDVLSEQTAKQGFYDRAS
2) FLLGPLAADNQPSPTTTQSSQQQPGGSTLSQANPGTPGTPATPLVVASV
3) MISHSSSRRPPSRPPAPSRGSSSHSVVPYPASDSYPAIDSRAHQNEVIDLTESDGGGMADIGRPAKRPRLDVGNEPPQSSLDHTHNQHQQQQYQNQDQPSSQHHVSSMTGETPESSMNPLDLMSSSSTEQTGRPPWSFKGEIPRSDAEAQPEASQWPQSSSLPPLPIRPWKYGPQERYLAGTTDSKGGKECGAVKTTP
212
1502
1
244
1550
198










Molecular Recognition Features






MoRF SequenceStartStop




1) ADIGRPAK
2) LPIRPWKYGPQERYLA
59
165
66
180