<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP07474

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDKYIDARFDRVEKALTTLIDSIAKYNPSTALAEDLLAADKELSQGLETLQTHQNNHLRILALRADVTALDAQIKSALLALAGSRRDILTTPATVFPEDSPNRYVRRQTHNYEFRYDQLMSYARRISRNTVPAPGQTDGAAFFGPFDGGEGAGGPAEAAATVGTSAATPAATPGPLNGVGTPSAAAAGGAQNPSQMELDTPVAAAGGAGSKAVGGGGSGGNTTAGTSAGTPGPLSGMAPPANVPDVLPADYQQYIAPAAGTVFFPWPMPDLMRTGALRSLQAVTDSQSEKLGGAASPSFSIRFVSLEEQQEEERQRAAREAEEREAWEERQRQMAEEAAAAGGFGGSQPTMAGAARPGGPAAAAAPAQFASTLDMDDD
Length378
PositionMiddle
OrganismSporothrix insectorum RCEF 264
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Sordariomycetidae> Ophiostomatales> Ophiostomataceae> Sporothrix.
Aromaticity0.06
Grand average of hydropathy-0.367
Instability index53.60
Isoelectric point4.73
Molecular weight39032.66
Publications
PubMed=27071652

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP07474
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      33.30|      10|      15|     312|     321|       1
---------------------------------------------------------------------------
  312-  321 (18.52/11.55)	EERQR.AAREA
  328-  338 (14.77/ 7.81)	EERQRqMAEEA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      81.10|      17|      17|     173|     189|       2
---------------------------------------------------------------------------
  173-  189 (34.25/14.32)	PGPLNGVGTPSA.............AAAGG
  193-  207 (24.59/ 8.09)	PSQME.LDTP.V.............AAAGG
  231-  260 (22.26/ 6.59)	PGPLSGMAPPANvpdvlpadyqqyiAPAAG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.29|      16|      18|      96|     111|       3
---------------------------------------------------------------------------
   96-  111 (31.35/22.72)	FPEDSPNRYVRRQTHN
  114-  129 (28.94/20.43)	FRYDQLMSYARRISRN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.32|      12|      18|     133|     144|       4
---------------------------------------------------------------------------
  133-  144 (22.51/ 9.03)	APGQTDGAAFFG
  152-  163 (22.81/ 9.24)	AGGPAEAAATVG
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP07474 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) APGQTDGAAFFGPFDGGEGAGGPAEAAATVGTSAATPAATPGPLNGVGTPSAAAAGGAQNPSQMELDTPVAAAGGAGSKAVGGGGSGGNTTAGTSAGTPGPLSGMAPPANVPDVLPAD
2) TGALRSLQAVTDSQSEKLGGAASPSFSIRFVSLEEQQEEERQRAAREAEEREAWEERQRQMAEEAAAAGGFGGSQPTMAGAARPGGPAAAAAPAQFASTLDMDDD
133
274
250
378










Molecular Recognition Features






MoRF SequenceStartStop




1) QPTMAGAARPGGPAAAAAPAQFASTLDMDDD
348
378