<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP07276

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAPIPQPSPLEGHQMAGAMVPAGQGGGGGGPGGSISLALLIDFIIQRTYHELSVLAELLPRKTDMERKIEIYQFASRTRQLFVRLLALVKWAASVSKVDKSANIMAFLDKQSMIFIETADALSRMARETLVHARLPNFHLPAAVEVLTIGTYSRLPLVIRDKIIPPDPITAIEKKNTLMRLSQVIEYRLATTTLPMQMRKNKIENGRVKFTVDHEFEASLTLMGDGPTVPWSLLDINILVEDKETGDGKNLVHPFQVRYIHELLQSRLRDCDNPLLELYTVLHTFSQSLQLEVLHSQTKRLCQERLGDLIRIEHYTEGRSLTISYWKDLASRELKSDLGYRLTAQVCTSHSGGTDVIGKPLQILHNPPLSTKDSEMAEQSIRSDQLSLEKLLTHTIYMRTRTRLLELKVELQPKLGHQQTDCVVQGSPPVLHLPILSPCLRSEQLLITVDTRTGSLQAHIPQDDPPASLMSELQNTLNSDKIRFEAAIAELRFWITQRRCQKTLQHLPVSVYDKLPLMYPADSPMSRLGKHKFFLRLHKHPNSWVVVEFKEKAKDIDYVCHLVVVKPVPIEENVSTDETSIELPKMYYKVMEVNELDMFLLTHGPCTALESQEVVGTKRKTGSTSGPLEVARDSTPGPCKRSKHPAYLLPDLSHVVALCDERIPFASLASQLTKREILHQGLTVEALGTGLSLKLVSLPPVEGVSREALNDLNRRLLTATIRMQVKGSRAWMMEFLFHGSPLVSVSPTEQGSRFPVYLSYDVTTADDAGRTVDAILQDWAHIANLYAAIRSLANVMQLKEERQHFSVKSYNFKKLILGYGPARMATAAVTWKSHERRFHLGLGCSGQLGSNPHSPLREQLEFFLNKERSCGYLARVLHETYEPLASLAKLPSTLQLGLLSTKPPIPVQTFTVMAHSPTHLRLAYCNLYCLDIHLQSDGQVSIRDGAFSVFDKSKVIEEFGPTQGLKAFLSKYVDETALSRRRSQSEDDHPPSPITMEHTNEPSHFLGHSRSTGAAGASPAGQQRGDLAGLRFQTPLTPSGTSNPHTPASPHMGNISQQQVQPQAQHGFGSSPATSSFSLASPPSLPPGAGVNPSPSMLPHPSPVGPGSAFGIASSPIPNPLHAPSPAGMLPTPSPNPAGMSGGAHNIESSPFPPQSLASPAPNTWPGSPGMPRPSPARPGGPPSAAASHHSPHGGHSGSAGGGFGGSFVSRVLPQRSWAGAVPTILTHEAFDVLCTPSTLPGEMDETFSAGGMMAPLERFLGCISMRRSLQRIIQDSQDCPSALPITEPGVIAFRCESLHGRVTVNAVHMQSLQLKLSPSGPEYREQWTMEEFQVLERFFEVRVMAIPFKPMAVHAFCRLLNAPHHILRDLVQLMKLELMPNAFGQQTFKWSVQFCLTVPPSAPSIVPIGASSVLTARNKMLFFLYIRRLGMLPEAEGASLVLPLVYDSINNVTQLAERRDAAAGNMGGLGPVAHTASMMLKRFGECSHMMGLQPGECSIYLAVRYLLANLTLPNEPPAMAVGPGNNGVELQQQPNQMVPMGLGSGPGMANMGPGTPINPMQMQAGPMSMGGGLPQQVQTQGQVSMPIHSPYGQAPMSANPMTPVMSMPINNTIGPTMGQGGTGNMQMQ
Length1629
PositionTail
OrganismDaphnia magna
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Crustacea> Branchiopoda> Diplostraca> Cladocera> Anomopoda> Daphniidae> Daphnia.
Aromaticity0.06
Grand average of hydropathy-0.194
Instability index56.95
Isoelectric point8.29
Molecular weight178064.10
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP07276
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|     331.99|      41|      41|    1114|    1154|       1
---------------------------------------------------------------------------
    5-   33 (29.64/ 6.88)	..P......QPSPLEGH.QMAGaMVPAGQ..GGGGGGPGG...........
 1010- 1038 (38.20/11.25)	RS.........TGAAGA.SPAG.Q...QR..GDLAGLR......FQTPLTP
 1039- 1062 (30.53/ 7.34)	.SG......TSNP.HTPaSP.................HMG..NISQQQVQP
 1070- 1112 (44.05/14.23)	SSPatssfsLASPPSLP.PGAG.VNPSPSmlPHPSPVGPG......SAFGI
 1114- 1154 (81.21/33.18)	SSP......IPNPLHAP.SPAG.MLPTPS..PNPAGMSGGAHNIESSPFPP
 1158- 1192 (62.67/23.73)	ASP......APNTW..P.GSPG.M...PR..PSPAR.PGGPPSAAASHHSP
 1545- 1575 (45.68/15.06)	..............SGP.GMAN.MGPGTP..INPMQMQAGPMSMGGG..LP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      85.26|      28|      41|    1237|    1271|       2
---------------------------------------------------------------------------
 1237- 1271 (43.02/38.68)	PSTLPgemdetFSAGGMMA.PLERFLG.....CISMRrSLQ
 1281- 1314 (42.24/21.57)	PSALP......ITEPGVIAfRCESLHGrvtvnAVHMQ.SLQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     688.78|     186|     372|     147|     361|       3
---------------------------------------------------------------------------
  147-  344 (290.66/220.17)	LTIGTYSRLPLVIRDKIIPPD.PITAIEKKNTLMR.LSQ......VIEYR............LATTTLPMQ....MRKNKIENGRVKFTV.DHEFEASLTLMGDGPTVpwSLLDINILVEDKE....TG...DG...............KNLVHPFQ.VRYIHELLQSRLRDCDN..PLLELYTVLH.TFSQSLQLEVLHSqTKRLCQERLGdliriehytEGRSLTISYWKDL..ASRELKSDLGYR.LTA
  507-  719 (224.96/143.34)	LPVSVYDKLPL.....MYPADsPMSRLGKHKFFLR.LHKhpnswvVVEFKekakdidyvchlVVVKPVPIEenvsTDETSIELPKMYYKV.MEVNELDMFLLTHGPCT..ALESQEVVGTKRK....TG...STsgplevardstpgpcKRSKHP....AYLLPDLSHVVALCDEriPFASLA.......SQLTKREILH...QGLTVEALG.........TGLSLKLVSLPPVegVSREALNDLNRRlLTA
  746-  896 (173.16/127.44)	SPTEQGSRFPVYLSYDVTTAD...DAGRTVDAILQdWAH......IANLY............AAIRSLANV....M...QLKEERQHFSVkSYNFKK..LILGYGPA...RMATAAVTWKSHErrfhLGlgcSG...............QLGSNPHSpLREQLEFFLNKERSCG......YLARVLHeTYEPLASLAKLPS.TLQL..............................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.97|      12|      27|    1491|    1502|       4
---------------------------------------------------------------------------
 1491- 1502 (24.38/12.82)	MGLQPGECSIYL
 1520- 1531 (21.59/10.46)	MAVGPGNNGVEL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     104.47|      33|      35|     428|     462|       5
---------------------------------------------------------------------------
  428-  462 (51.60/34.88)	PPVLHLPILSPCLRSEQllITVDTRTGSLQAHIPQ
  465-  497 (52.86/30.31)	PPASLMSELQNTLNSDK..IRFEAAIAELRFWITQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     121.89|      37|      51|    1348|    1385|       7
---------------------------------------------------------------------------
 1348- 1385 (62.81/43.24)	PFKPMAVH.AFCRLLNAPHHILRdLVQLMKLELMPNAFG
 1401- 1438 (59.09/35.74)	PSAPSIVPiGASSVLTARNKMLF.FLYIRRLGMLPEAEG
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP07276 with Med14 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) PPAMAVGPGNNGVELQQQPNQMVPMGLGSGPGMANMGPGTPINPMQMQAGPMSMGGGLPQQVQTQGQVSMPIHSPYGQAPMSANPMTPVMSMPINNTIGPTMGQGGTGNMQMQ
2) TALSRRRSQSEDDHPPSPITMEHTNEPSHFLGHSRSTGAAGASPAGQQRGDLAGLRFQTPLTPSGTSNPHTPASPHMGNISQQQVQPQAQHGFGSSPATSSFSLASPPSLPPGAGVNPSPSMLPHPSPVGPGSAFGIASSPIPNPLHAPSPAGMLPTPSPNPAGMSGGAHNIESSPFPPQSLASPAPNTWPGSPGMPRPSPARPGGPPSAAASHHSPHGGHSGSAGGGFGG
1517
976
1629
1206










Molecular Recognition Features






MoRF SequenceStartStop




NANANA