<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP07228

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDDILQAQFDRVEQALSTLVDSIASYNPNPQAAIDLVAADDELSHGLDQLARHQANHARIQTLRAEADALEEQLKSSVAALAGLRKELSETPATVFPEDSRPVPFDELLGYAKNISKYTVPPTFRERVPEPELESHKDKDAFEGSASAPATNGVNTPTTAAAAVEAPKGNIEPPKEGAGTDAAAPEITAEEEEWLKKLKDSGFAWFPWPDDAKIRRGNLWKVYHYREHGRNLDEFNIEAYEEEEKKKFLDAVQPQQAPEEMQAEPQFQEQRAQQAPQAQAAPAAPAGAGFTLFDDMESDED
Length301
PositionMiddle
OrganismDidymella rabiei (Chickpea ascochyta blight fungus) (Mycosphaerella rabiei)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Dothideomycetes> Pleosporomycetidae> Pleosporales> Pleosporineae> Didymellaceae> Ascochyta.
Aromaticity0.07
Grand average of hydropathy-0.736
Instability index59.59
Isoelectric point4.50
Molecular weight33191.04
Publications
PubMed=27091329

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP07228
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.77|      15|      16|     250|     265|       1
---------------------------------------------------------------------------
  250-  265 (23.10/16.13)	DAVQPQQAPEEmQAEP
  268-  282 (25.66/13.10)	QEQRAQQAPQA.QAAP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      77.28|      24|      24|       6|      29|       2
---------------------------------------------------------------------------
    6-   29 (41.10/26.70)	QAQFDRV..EQALSTLVDSIASYNPN
   31-   56 (36.17/22.77)	QAAIDLVaaDDELSHGLDQLARHQAN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.72|      13|      16|     148|     161|       3
---------------------------------------------------------------------------
  148-  161 (18.34/16.04)	APATNgVNTPTTAA
  166-  178 (24.38/15.49)	APKGN.IEPPKEGA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      74.24|      22|      24|      81|     104|       4
---------------------------------------------------------------------------
   81-  104 (33.83/30.14)	LAGLRKELSEtpATVFPEDSRPVP
  108-  129 (40.41/27.92)	LLGYAKNISK..YTVPPTFRERVP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP07228 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) EEKKKFLDAVQPQQAPEEMQAEPQFQEQRAQQAPQAQAAPAAPAGAGFTLFDDMESDED
2) VPPTFRERVPEPELESHKDKDAFEGSASAPATNGVNTPTTAAAAVEAPKGNIEPPKEGAGTDAAAPEITAEEEE
243
120
301
193










Molecular Recognition Features






MoRF SequenceStartStop




1) AGFTLFDDMESDED
2) KKKFLDA
3) QAEPQFQEQRAQQAPQAQAAPA
288
245
262
301
251
283