<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP07195

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMNENNKKMQLELLMEKLRHKTSHLNLTDSAKTFRMTILEKRYNNDTVEKLQYQKCLDLLQTNMKVTSLQSMLERLESISRQAGLKFTADGSGRAFFISSDMFYLEVIVEQGGSVCEIHIQHEGKSEPQTCNEIAVCLNSGNFSKFTNHLQGLCAIYQVSADKKLKAKAFAALSALELDLGSMSKLQVYRQDIAMLVHKSPLGIVMPRVGGLPLTLTYFLSPYDLVNVQSPTLTMTLEKILEKNAGLSCTIGIDSANNFQLPISPLFSVTRNSEGESVPAFSPLNAVNSASLPATFVLRMRDPLVLCFSFAQQIKSITGMEVGNWDQPEHLFRSLIKTISKGLIECSGIEDGISRALHVTLPDQQHSYFITESEDAALKCCSVSSIPFSHPSHVPQILAILRRQGLLNALLSSCIRPKSQSDSDAALTFEINSIALDHMSVTFEHPLLEQMCSVEIDLGMDPTCIRCSLFGAEDERTSSDEYATRVLQRCLSIPVTMRAILRQMQSPSSRWSNGPGDPQGSFERRFQTHTNGDSDSHTGPNGQYGNPSFTGGGGGGGFDSGHSPRTHNIGNNDVMMHQSMSNPHASTPKETENESFNGEMEQPGEVVSLAEDGTSANRSKAQFLPASESRTSTSTLGNADLTKYSVNSHSSDSILGITLNNSSTQFNPVRRLNNDVSLKSEPVDMEVSVLEPTTENNQIITEDADSLSSNDAVASLLNVSKSRESPSPKSKSAYPSVSITPVNSLHTSSHTAAYPNINLERRPGIEIIPFSKDSSSSSIPSSLTITPVGGKPPKEKSKYKDFRLKESLALDSETKERDRDKERERKERKRRREGEKSPSSGSGGKPTKMMSLSATLMGPPGALLKLDSSPKLSHKNNSSGSNSGSTPPANTNQSPLTSPSKSSSKSSTPSSSPKHPSSGGKPSMSALSMSALKCSTSPSSKSKKSKDRDRERSDKDRDRDKDRERDRDKDRERDRERDKDRSRDREKKSRTPTPTSNHHSRGPPLLKTKSLDFALAVEDKSEETKEDNSFDSILALATANSEMLPSQLDLQSSIHAVTQELLSFSPPTLSDSENGKVSGSTGGLPGDGSMSCPSSGNPNKSPLRSRKSSLSAVIDKLRLQHNPESDVFQNSGPKYGADPASRMEQSEGRSSFMDELMEGSDSSASPKTNDVEDNMHFNRIHSGSNSPNDPFSQEDSGINETTDAHEGECNGDSLPDKEFVKPFPPPSARGTYGIRPHGYLSNSEADLNPNSPSVSVHIVNVPSPLALQQTRSPLANQLALVTATSGPSPASSTPQSSPCIIDDDLMDEALVGLGK
Length1314
PositionMiddle
OrganismDaphnia magna
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Crustacea> Branchiopoda> Diplostraca> Cladocera> Anomopoda> Daphniidae> Daphnia.
Aromaticity0.04
Grand average of hydropathy-0.642
Instability index59.15
Isoelectric point6.12
Molecular weight142802.59
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP07195
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     157.18|      21|      73|     825|     845|       1
---------------------------------------------------------------------------
  519-  546 (22.41/ 6.63)	G..SFERRfqTHTNG.DSDS.....H...............TGpngqyGNP
  825-  845 (34.62/15.08)	K..ERKRR..REGEK.SPSS.....G...............SG.....GKP
  866-  886 (29.54/11.56)	D..SSPKL..SHKNN.SSGS.....N...............SG.....STP
  896-  921 (23.71/ 7.53)	T..SPSKS..SSKSS.TPSSspkhpS...............SG.....GKP
  968- 1003 (23.55/ 7.41)	K..DRERD..RERDK.DRSR.....DrekksrtptptsnhhSR.....GPP
 1166- 1189 (23.35/ 7.28)	KtnDVEDN..MHFNRiHSGS.....N...............SP.....NDP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     164.24|      43|      54|    1050|    1095|       2
---------------------------------------------------------------------------
 1050- 1095 (67.37/38.02)	QSSIHAVTQEL.LSFSPPTlsDSENGKVSGSTGGLPGDGSMScPSSG
 1106- 1147 (67.20/29.51)	KSSLSAVIDKLrLQHNP....ESDVFQNSGPKYGADPASRME.QSEG
 1222- 1243 (29.68/ 9.18)	..............FPPPS........ARG.TYGIRPHGYLS.NSE.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     148.30|      33|     139|     792|     824|       3
---------------------------------------------------------------------------
  727-  766 (42.90/24.15)	PKSKSAYPSVSITpvNSLHTSSHTAAYpninlERRPGIEI
  792-  824 (57.08/34.86)	PKEKSKYKDFRLK..ESLALDSETKER.....DRDKERER
  937-  964 (48.32/28.24)	PSSKSKKSKDRDR..ER....SD.KDR.....DRDKDRER
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     124.47|      30|      66|     565|     594|       4
---------------------------------------------------------------------------
  565-  594 (54.59/31.75)	THNIGNNDVMMHQSMSNPHASTPKETENES
  632-  661 (46.61/25.95)	TSTLGNADLTKYSVNSHSSDSILGITLNNS
  668-  685 (23.28/ 8.99)	VRRL.NNDVSLK........SEPVDME...
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     133.78|      43|     868|     375|     420|       6
---------------------------------------------------------------------------
  375-  420 (71.64/52.88)	AALKCCSVS.SIpfsHPSHVPQILAILR.RQGLLN..ALLSSCIRPKSQS
 1244- 1290 (62.14/37.21)	ADLNPNSPSvSV...HIVNVPSPLALQQtRSPLANqlALVTATSGPSPAS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.83|      17|      19|    1006|    1022|      10
---------------------------------------------------------------------------
 1006- 1022 (26.17/19.26)	KTKSLDFALAVEDKSEE
 1025- 1041 (26.66/19.77)	EDNSFDSILALATANSE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      70.12|      21|      65|     704|     725|      11
---------------------------------------------------------------------------
  611-  631 (36.29/16.31)	DGTSANRSKAQFLPASESRTS
  704-  724 (33.83/19.73)	DSLSSNDAVASLLNVSKSRES
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP07195 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DAVASLLNVSKSRESPSPKSKSAYPSVSITPVNSLHTSSHTAAYPNINLE
2) DSILGITLNNSSTQFNPVRRLNNDVSLKSEPVDMEVSVLEPTTENNQIITEDAD
3) RQMQSPSSRWSNGPGDPQGSFERRFQTHTNGDSDSHTGPNGQYGNPSFTGGGGGGGFDSGHSPRTHNIGNNDVMMHQSMSNPHASTPKETENESFNGEMEQPGEVVSLAEDGTSANRSKAQFLPASESRTSTSTLGNADLTKYS
4) SSSIPSSLTITPVGGKPPKEKSKYKDFRLKESLALDSETKERDRDKERERKERKRRREGEKSPSSGSGGKPTKMMSLSATLMGPPGALLKLDSSPKLSHKNNSSGSNSGSTPPANTNQSPLTSPSKSSSKSSTPSSSPKHPSSGGKPSMSALSMSALKCSTSPSSKSKKSKDRDRERSDKDRDRDKDRERDRDKDRERDRERDKDRSRDREKKSRTPTPTSNHHSRGPPLLKTKSLDFALAVEDKSEETKEDNSFDSILALATANSEMLPSQLDLQSSIHAVTQELLSFSPPTLSDSENGKVSGSTGGLPGDGSMSCPSSGNPNKSPLRSRKSSLSAVIDKLRLQHNPESDVFQNSGPKYGADPASRMEQSEGRSSFMDELMEGSDSSASPKTNDVEDNMHFNRIHSGSNSPNDPFSQEDSGINETTDAHEGECNGDSLPDKEFVKPFPPPSARGTYGIRPHGYLSNSEADLNPNSPSVSVHIVNVPSPLALQQTRSPLANQLALVTATSGPSPASSTPQSSPCIIDDD
710
651
501
775
759
704
644
1303










Molecular Recognition Features






MoRF SequenceStartStop




1) DRERDRDKDRER
2) FALAV
3) GTYGIRPHGYL
4) KEKSKYKDFRLKESLAL
5) VIDKLRL
961
1012
1229
793
1112
972
1016
1239
809
1118