<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP06556

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMHASGEPAAAPHSASPTSLPPSNGTRANQDQDGDVEGGDERELPVVLQDLVPLSYLIDRVVSSAYSDLATLVETLPGSHSHASTSAAGTDDQARKRKIVDHVLNSRRQLVKLLVLARWSKEAHRLHKAINIVGFLAIQNHQIDRAIEALTETHGMLARARVRNYDLASALSVLSTGSAASLPASLTDPFAADLAHPLTDAQVLDTLAELDRVLLARLVLRIEPLPPALTDPAAWRIHDGRVTFRVDGMWEADLTFGGDNESEESTGQQGGEWYLLAIRFLFRVKDARGAWSSTPLGPIKDHLLQLCNQELLRRPYFPLPLPPRPTFALDPHSTSATTGGAGRETGTTPLLPDPQAPPPAPGEMPVLFPNGDSSAAPNGVIMGMGGEDNKSEQERVRQEWDEKEREWQREKDEVIKKRKRDRPLDRGYTFLQRLALSYQLEAVHSSAARLAATSWSGSLEVERRTSKTTTAGAGNAEVEGEDEVRIHYWRPPAAISSSAAAATSGKPLRPTSTPAPSTSTAAQPGPPAQPGRRAGPGGTVVFSLRPIPQVAVTGGGGGPRRGGLSRMRGERARQDALQAALDRAARSASASSFGSGGGGGDDDELEDPMPASQTPPTRIDVPKTLSVMWLPSTTNGTPASIPSALASLNLTSLLGSDLDVEGVLRHVTALHARDAIERLSTVILAAPTPGSGDEAKKHERMQRARLVYPSSRRRRRPVGLAGSETKNKEVEEEDLTDVVVPYLYLPLVGSHAIGAHIDPVTGRFELRVAPAIAPDAGSLNGRVDFAEDEDEGSAAEVEGGSSARDQRLRLASERIDRERFGTAVVGGPKGVKQQQQQQQGDPDAWMKGVVEVVARTRASTIIDELDTLLSLLSLPLSSPPVRRLPLPPRELAKLGPNASAAAGRAAFLFVPLLADEPALAGWFLVLELFEDGIRAALLSTAERSDQLGNWTEILEVGWLGTPSPAAATSEAVEAKTAETAAGHAKGANLGFEIKSEVLRSLWWHCVRRAAAFSLELQLHLRRIPYRRESVSSVATASRFVLPTANLLRIPDVEQLLRPDAELRCAVEEDGGRKTILLVYLRITPGAPSPLPPPNELPPSVLYNPARGALVLVAEGSFADTVERLLRALATVLKLVRSARPLGSPAKALSASARLKTVKVEKH
Length1161
PositionTail
OrganismRhodotorula sp. JG-1b
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Pucciniomycotina> Microbotryomycetes> Sporidiobolales> Sporidiobolaceae> Rhodotorula> unclassified Rhodotorula.
Aromaticity0.05
Grand average of hydropathy-0.304
Instability index49.81
Isoelectric point6.30
Molecular weight124503.19
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP06556
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     190.48|      33|      40|     310|     343|       1
---------------------------------------------------------------------------
  310-  342 (61.74/20.50)	LLRRPYFPLP.....LPPR.PTFALDPHSTSATTG...GAGR
  349-  385 (52.32/16.00)	LLPDPQAPPP.....APGEmPVLFPNGDSSAAPNGvimGMGG
  870-  903 (43.14/11.63)	LLSLPLSSPPvrrlpLPPR.ELAKLGPNASAA.......AGR
 1083- 1107 (33.28/ 8.84)	....PGAPSP.....LPP..PN.ELPPSVLYNPAR...GA..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.26|      18|      40|     181|     202|       2
---------------------------------------------------------------------------
  181-  202 (24.03/23.76)	LPASLTDPfAADLAHpltDAQV
  224-  241 (37.23/20.27)	LPPALTDP.AAWRIH...DGRV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     184.01|      48|      91|     473|     526|       3
---------------------------------------------------------------------------
  473-  526 (73.60/36.80)	GNAEVEGE....DEVRIHYWRPpAAISSSAAAATS...GKPLRPTSTPAPSTSTaaqpgPP
  528-  560 (44.11/12.56)	QPGRRAGP....GGTVVFSLRP...IPQVAVTGGG...GGPRR..................
  562-  615 (66.30/23.38)	GLSRMRGErarqDALQAALDR..AARSASASSFGSgggGGDDDELEDPMPASQT.....PP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     156.02|      41|     128|     637|     678|       5
---------------------------------------------------------------------------
    7-   54 (38.97/14.36)	PAAAPHSASPTSLPPSN......GTranqdqDGDVEGgderelpvVLQDLVPLS............
  637-  677 (69.88/34.14)	PASIPSALASLNLTSLL......GS......DLDVEG........VLRHVTALHARD.....AIER
  769-  813 (47.17/19.60)	PAIAPDA.GSLNGRVDFaededeGS......AAEVEG..............GSSARDqrlrlASER
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.76|      13|     126|     258|     272|       6
---------------------------------------------------------------------------
  258-  272 (20.57/23.18)	DNESEESTGQQggEW
  387-  399 (25.19/18.21)	DNKSEQERVRQ..EW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.50|      18|      41|     951|     968|      14
---------------------------------------------------------------------------
  951-  968 (32.43/14.71)	EILEVGWLGTPSPAAATS
  995- 1012 (35.07/16.40)	EVLRSLWWHCVRRAAAFS
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP06556 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AATSWSGSLEVERRTSKTTTAGAGNAEVEGEDEVRIHYWRPP
2) ISSSAAAATSGKPLRPTSTPAPSTSTAAQPGPPAQPGRRAGPGGTVVFSLRPIPQVAVTGGGGGPRRGGLSRMRGERARQDALQAALDRAARSASASSFGSGGGGGDDDELEDPMPASQTPPTRIDVPKTLSVMWLPSTTNGTPASIPSALA
3) MHASGEPAAAPHSASPTSLPPSNGTRANQDQDGDVEGGDER
4) PPRPTFALDPHSTSATTGGAGRETGTTPLLPDPQAPPPAPGEMPVLFPNGDSSAAPNGVIMGMGGEDNKSEQERVRQEWDEKEREWQREKDEVIKKRKRDRP
450
494
1
321
491
645
41
422










Molecular Recognition Features






MoRF SequenceStartStop




1) VRIHYWR
483
489