<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP06514

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAEIGQESVEFSTLVSRAAEESYLSLKELVEKSKSSDMSDSEKKISILKYLVKTQQRMLRLNVLAQWCQQVPLIQYCQQLASTLSSHETCFTQAADSMFFMHEGLQQARAPIYDVPSAIEVLLTGTYQRLPKCTEDVGIQSTLTDKQQKPVLKKLDTIVRSKLLETSLPKEFSEVKIFDGTVHLCVQGEFKVLLTLGYRGHLSMWRILHLEVLVGERSGLVKLEETRRFVLGDDLERRMAASDSPFATMYSILHEFCVALIMDTVIRQVQALRLGRWKDAIRFELISDGNPGQGSATGSVQMNQDGETDSGGLRTPGLKILYWLDCDKNNGTSDAVSCPFIKIEPGQDLRIKCLHSAFVIDPLTNKEAEFSIDQSCIDVEKLLLRAIWCNKFTRLLEIYKELGENSHIRRAAGDVLLHCPVDQPGAEYKACIYQVVEVVSVVGGGLHLVLVGLVGDVADNKSDSEGQEVLRVRAYGSSFFTLGINIRTGRFLLHSSRSTIAPSALRECEEALNQGSITAAEAFISLRSKSFLHLFACIGRFLGLQVAVFLACKRKKKKPILLLVYEHGFSPVKVPKHILNGSNMLLMGFPDCGSSYFLLMQLEEDFRPLFKLLETQADPPGKPESSGVLNQVTRIKNVDVNQMHMLEDELNLSLLDYGALTLPANAVGTNQTSENGLSDLSVEGSGLLSAFSSIVDEVFEQERRSSAPAFPVQSFNTTFNTTTNHGSGPTISSSPKWEGGPQMSHIDNPAGSSWNGALYPTSNYKGRIPSVSTSSIAPAPGRSTVMKKLPASKSDQDLASRSPHSAEVGSYITMEEDQLGVAGSRSSRLSSLPQSSGPRVSAASARANAFRNSVTGTPAPAPDSPMCTSSLPAVVSKYDNGSRKRALMDMLDLLPSLKCPEILEGSFKRRKITNSVSTQRSSLQTQSPLGVVNNIEKYRYVNLIAEANKGDAPSSIYVSALLHVVRHCSLCIKHARLTSQMDELNIPYAEEVGLRNASSNIWFRLPFARGDAWEHICLRLGRPGSMYWDVKINDQHFMDLWELQKGSTSTPWGSGVRIANTSDIDSHIRYYADGVVLTYNSVESDSIKKLVADIQRLSNARTFALGMRKLLIIKSDEKTEESRSSSDAKAPPGAKSTSEAVDKYLEQMRRAFKIEAVGLMSLWFSFGSGVLARFVVEWESGKSGCTMHVSPDQLWPHTKFLEDFINGTEVSSLLDCIRLTAGPLHALAAATRPARAAPVSGVPGSASSVPTLSKASVYGQMPNNSNNVGHSSAPGGNPGASAISTLGTHGHHGSAMLTAASAAGRGGPGIVPSSLLPIDVSFVLRGPYWIRIIYRKYFAVDMRCFAGDQVWLQPATPPKGGPTVGGSLPCPQFRPFIMEHVAQELNGLDPNFTGGQQSVGPTNSNNSGAPSSPQVSASNGNRVSLAGPTGMSRPGNQPVGFSRATNAMPTSSTSSLGSSGLALRRPPGTVVPAHVRGELNTAIIGLGDDGGYGGGWVPLVALKKVLRGILKYLGVLWLFAQLPELLKEILGSILKDNEGALLNLDQEQPALRFFVGGYVFAVSVHRVQLLLQVLSVKRFHHSQQQQQNSVIAQEELSQSEIGEICDYFSRRVASEPYDASRVASFITLLTLPISVLREFLKLIAWKKGLVQTQGGDIPPAQRSRIELCLENHSGLYTDGNSDNSSVSKSNIHYDRPHNAVDFGLTVVLDPAHIPHINAAGGAAWLPYCVSVRLKYSFGDNPSVCFLGMEGSHGGRSCWLRLEDWEYCKQRVIRMVDMNGSSGGDANQGRLRMVADNVQRALHLCLQGLRTGGGGSSGGGGGVATGGNTT
Length1829
PositionTail
OrganismCynara cardunculus var. scolymus (Globe artichoke) (Cynara scolymus)
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Streptophyta> Embryophyta> Tracheophyta> Spermatophyta> Magnoliopsida> eudicotyledons> Gunneridae> Pentapetalae> asterids> campanulids> Asterales> Asteraceae> Carduoideae> Cardueae> Carduinae> Cynara.
Aromaticity0.07
Grand average of hydropathy-0.165
Instability index49.86
Isoelectric point8.12
Molecular weight198644.96
Publications
PubMed=26786968

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP06514
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     209.32|      43|      66|     769|     811|       1
---------------------------------------------------------------------------
  729-  759 (36.41/14.43)	.........PTISSSpKW.............EGGPQMSHIDN..PAGSSWN....GALY
  760-  802 (72.06/35.42)	PTSNYKGRIPSVSTS.SI.............APAPGRSTVMKKLPASKSDQ..DLASRS
  803-  835 (39.95/16.51)	PHSAEVGSYITMEED.QL.............GVAGSRSSRLSSLPQS............
  838-  883 (30.90/11.18)	.........PRVSAA.SAranafrnsvtgtpAPAPDSPMCTSSLPAVVSKY..DNGSR.
  895-  927 (30.00/10.65)	.........PSLKCP.EI.............LEG...SFKRRKITNSVSTQrsSLQTQS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     281.33|      91|     324|      38|     153|       2
---------------------------------------------------------------------------
   38-  138 (138.44/106.92)	MSDSEKKISILKYLVKTQQRMLRlnvlAQWCQQVP.LIQYCQQLAStlSSHetcFTQAADSMFfMHEGLQQA....RAPIYDVPSAIEVLLTGTYQRLPKCTEDVG
  363-  458 (142.89/84.21)	LTNKEAEFSIDQSCIDVEKLLLR....AIWCNKFTrLLEIYKELGE..NSH...IRRAAGDVL.LHCPVDQPgaeyKACIYQVVEVVSVVGGGLHLVLVGLVGDVA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     136.28|      39|     324|    1457|    1495|       3
---------------------------------------------------------------------------
 1457- 1495 (70.73/34.86)	GSSGLALRRPPGTVVPAHVRGELNTAIIGL..GDDGGYGGG
 1779- 1819 (65.55/31.80)	GSSGGDANQGRLRMVADNVQRALHLCLQGLrtGGGGSSGGG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     185.69|      56|     324|     653|     728|       4
---------------------------------------------------------------------------
  663-  727 (85.54/72.25)	PANAVGTNQTSENGLSDLSVEGSglLSAFSSIVDevfeqerRSSAP.AFPVQSFNTTFNTTTNHGS
 1238- 1294 (100.15/50.13)	PVSGVPGSASSVPTLSKASVYGQ..MPNNSNNVG.......HSSAPgGNPGASAISTLGTHGHHGS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     187.18|      59|     127|    1031|    1104|       8
---------------------------------------------------------------------------
 1037- 1102 (89.35/71.12)	FMDLWELQKGSTSTpWGSGVRIANTSDI....DSHIRYYADGvvltyNSvESDSIKKLVADIQRLSNART
 1639- 1701 (97.84/50.58)	FLKLIAWKKGLVQT.QGGDIPPAQRSRIelclENHSGLYTDG.....NS.DNSSVSKSNIHYDRPHNAVD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     116.58|      37|    1086|     490|     530|      10
---------------------------------------------------------------------------
  490-  530 (51.82/45.47)	RFllHSSRSTIAPSALreCEEALNQGSITAAEAFISLRSKS
 1578- 1614 (64.76/42.97)	RF..HHSQQQQQNSVI..AQEELSQSEIGEICDYFSRRVAS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     114.91|      35|      37|    1375|    1411|      12
---------------------------------------------------------------------------
 1375- 1411 (58.50/38.02)	PFIMEHVAQELNGLDPnfTG....GQQSVGPTNSNNSGAPS
 1413- 1451 (56.41/30.43)	PQVSASNGNRVSLAGP..TGmsrpGNQPVGFSRATNAMPTS
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP06514 with Med14 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AFPVQSFNTTFNTTTNHGSGPTISSSPKWEGGPQMSHIDNPAGS
2) AGSRSSRLSSLPQSSGPRVSAASARANAFRNSVTGTPAPAPDSPMCTSSLPAV
3) GLDPNFTGGQQSVGPTNSNNSGAPSSPQVSASNGNRVSLAGPTGMSRPGNQPVGFSRATNAMPTSSTSSLGSSGLAL
4) PSVSTSSIAPAPGRSTVMKKLPASKSDQDLASRSPHSAEVGSYITMEEDQLG
709
822
1387
769
752
874
1463
820










Molecular Recognition Features






MoRF SequenceStartStop




NANANA