<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP05446

Description Mediator of RNA polymerase II transcription subunit 13
SequenceMTHQNHQTNGASLEDCHTNFYALTDLCGIKWRKFVMEERPNALSDPLKDPILRSYSRCIQADMLCVWRRVQSTKQDNTDPNALTYEITTSTKIHPPLSLAAAKELWIFWYGEEPDLSELVDADLLRVAANQALWNGTWKGALTYECRSLLFKALHNLMERFVLTKDIVRFGKWFVQPCTSSDRLFGRSSQHLSFSFTFFVHGDTVCASIDLREHPAVRPLTKEHLAEAAAAFAAASAPPGSAAPGAAGGAGGPQPSQEPAQNGPAMDEAGEGGAAKVTPPAHPRKVMLAPFGIAAILTGNSYKANDPMAEKILEDWASFFPLCNKDNTDLPPVVEVVSGGHKMYHPTNYVLVTDLDDMEHMEHMAELQKMQMHNAAGHGGSEASLSCPPVAGAAAGPVPGAPGTAAVPVGPASASASAKEASTRKTAPQVVSALERLSFQPYYDQRPTSGFTFNTNNTHIPATAAVEMPERTWQDCIMNTLHVDAAAAAAAAAASSRSTGPEASAAGEEEDSKQQVQQQLQQQQQQQQQRHKLWNFVDPMQKAPCTCTKHLGNTPHGTPHGGASTYSRNSLGGDSSMPVASVESPATPAPSPHPNSAHSQPTSVPPPEQMLNMSPHAPTSVSNLQQPPTPIDHLLDKNTPAPTPTDQHDSKSITASPYVHQTPSVEPPSYADHATGGGTGGSGSGGGATGTGPGSVPAQQPATPTAAAVPSAGAGSASGGAGSVGTISVKKLEMQQKQPPLTAAMAIKQEPGIAAERGGASGGGAASTAEAIQNLLSLYKPPQLTLKDEDSYYDEDWLKELYNFQCQENWDYLPVKRPKLEDTNKQRRPRYAKDLYEGHTHVKPLMPSPGSVYGSQLLSLDVQSNATGGGGAGTGGSGGTSGGLVGIANSNANGSDAENESGNSFFQGLDIKTEPGLHSPTKTSSGGGGGGSGGGSGGGNLFTAEGLNPSLNDLEQLFETSSNDECSSVQIHTPPDSNNPSNGGCSTVTNTIDELKRSTAISTNALVAAAAAAAAGNIQAEDLTKMFPTPPSHEQQHPNSSPCQTDVVMTDLSVDTSTSCISNSACITSSNTTIVSNNNNNSNVTINATTTTSSIILAAIQAPVAVVNVTKLVKQEYNLELGSPVEEAIDNWNYVYRPPQQEKFVGASKYAPLTNLPSQALPPLNIPPGCFYQPTWSSHKSRAATLAKAAAAQKQQHQKHQALQQRIQLHQQKLQQHQHQNHHQQQQQQQQQKHQHQQLHELLSAAPRTPMTPTVPQPLSSGGSQLLLNQLNCPQAPPGSSMQQMMRAGMSPISPGPGVVPFPRSSPMMRQTPTHPPPPYPYELAVASPATSTSSYLNRPLHSQEHQGMGGGGMGVGGMGAPGGGMSMMPYTGDAGISSGNSAAAVASSLLQELPEVNSVLVNILLYDTALNVFRDHNFDSSSVCVCNADTQKIGNIRGADSGVYVPLPGASFNPFPAGGGQRLLNGPAASFGGLRMISAFGGSPASASMPGAGSGHGHGPNGGSNSSSCTPPSSNPHITGYVDDDPVECTCGFSAVVNRRLSHRAGLFYEDEVEITGLADDPGRNKQPTLLSLIQSLCRKNQIKQAPGEPSSALEKAGSGGGAGATAGQLEHLAHAIFDLLLDQCSIIQTSSSSVHRALQSHRRRMSRHRRIFGTNGAPTASRASIANVLEFIDAHDVVSLALEQARLAFENNMMDFHGNGSSTFQAFHAPPPALRHKLAGIGGGRLTVHKWPYLPVGFTRSNKEIVRTMNAIQPMLQSAFHCKSRGGAAGSKDASSYNTVSGPLTWRQFHRLAGRASGQCEPQPIPSVIVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNDHVANSTRTYFRELSSTYEMCKLGRHTPIRGWDGILQVGAARNPAAADREATPLDEWLRSLEHAALAEQIRRYAVAFIHQLAPYLSRVPNDKTLLNPPDGTGNSGNTKGGSSLTPNLSAATGLPGGDLATDNIKLEPGTEPPPVEANAEVKQEPGTGKPGAAGADTKPALILGDPLGVGETLEDINPPAIVLYVVNPFTFGSDSCELERLALIALLRCYAELLKAVPDSVRSQMNIQIISLESVMELGPCGNRKRFSDEIKCLALNIFSQCRRHLVHAQSVKSLTGFGTAANMEAFLKTKDEPNRRAYKMYTAPFVLAPMHERNDKTDFSRSAGSMHGQNEHRYSVMYCNYCLSEDQAWLLATATDERGELLEKICINIDVPNRARRRKAPARYVALKKLMDFIMGLISQTSQMWRLVIGRIGRIGHSELKSWSYLLSKQQLQKASKQFKDMCKQCTLMYPPTILSACLVTLEPDAKLRVMPDQFTPDERFSQISMQNPLSTPQDVTCTHILVFPTSAVCAPFTRQFQNEPQVEDDFLTFEEEGNEDFDDADMNDLFWDPPMDKTSNHGSPGRMDDNRSWQSAGGNNFKCTPPQEVEEVGSLNQQPISVGYMVSTAPTGRMPAWFWSACPHLEDVCPVFLKTALHLHVPSIQTADDILNSTNAHQSANDHPLDSILTADVLRFVLEGYNALSWLALDSNTHDRLSCLPINVQTLMDLYYLTAAIA
Length2577
PositionMiddle
OrganismDrosophila ananassae (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila> Sophophora.
Aromaticity0.06
Grand average of hydropathy-0.403
Instability index51.36
Isoelectric point6.04
Molecular weight276017.26
Publications
PubMed=17994087

Function

Annotated function Component of the Mediator complex, a coactivator involved in regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP05446
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             7|    1103.56|     192|     194|     499|     690|       1
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  212-  307 (59.47/19.24)	..................................................................................................................................................REHPAVRPlTKEH..............LAEA..AAA...F...A..AA.SAPP..GSAA......PGAAGG...AGGP.QPSQ.EPAQ........................................NGP..AMDEAGEG...GAAKvtppaHPRKVM.................L.............................APFGIAA.ILtGNSykandP
  374-  555 (234.99/102.56)	NAAGHGG..........SEASL...SCPP..VAG.........AAAGPVPgA..PGTAAVP.......VGPASASASAKEAS.TRKTAPQVVSA.......................LER..LSFQP................YYDQ.RPTSGF.TFNT..............NNTHIPA.........T..AA.VEMPerTWQDcimntlH.VDAA...AAAA.AAAA.SSRS........................................TGP..EASAAGEE...EDSK.....QQVQQQ.................LQQQQQQ....QQQRH...KLWNFVDPMQKAPCTCTK.HL.GNT.....P
  556-  713 (266.63/117.58)	HGTPHGGA...STY...SRNSLGGDSSMP..VASVE.......SPATPAP.S..PHPNSAH.......SQPTSVPPPEQMLN.MSPHAPTSVSN.......................LQQPPTPIDH................LLDKNTPAPTP.TDQH..............DSKSITASP...YVHQT..PS.VEPP..SYAD......HATGGG...TGGS.GSGG.GATG........................................TGP..........................GS.................V.........................PAQQPATPTAA.AV.PSA.....G
  714-  977 (166.98/70.28)	AGSASGGAgsvGTI...SVKKLEMQQKQPplTAAMA.......IKQEPGI.A..AERGGA........SGGGAASTAEAIQNlLSLYKPPQLT........................LKDEDSYYDEdwlkelynfqcqenwdYLPVKRPKLED.TNKQrrpryakdlyeghtHVKPLMPSPgsvYGSQL..LS.LDVQ..S.........NATGGGgagTGGSgGTSG.GLVGiansnangsdaenesgnsffqgldiktepglhsptktssgGGG..GGSGGG.S...GGGN.....LFTAEG.................LNPSLND....LEQ.........LFETSSNDECSSVQiHT.PPD.....S
 1122- 1256 (88.53/33.03)	.........................................................................................................................GSPVEE................AIDNWNYVYRP.PQQE................KFVGASK...YAPLTnlPSqALPP.lNIPP......GCFYQP...TWSS.HKSR.AAT............................................laKAAAAQKQ...QHQK.....HQALQQriqlhqqklqqhqhqnhHQQQQQQ....QQQKHqhqQLHELLSAAPRTPMTPT.......V.....P
 1257- 1453 (174.39/73.79)	QPLSSGG....SQL...LLNQLNCPQAPP..GSSMQqmmragmSPISPGP.GvvPFPRSS..................PMMR....QTPT.............................HPPPPYPY................ELAVASPA.T.........................STSS...YLNR........PL..HSQE......H.QGMG...GGGM.GVGGmGAPG...........ggmsmmpytgdagissgnsaaavassllqELP..EVNSVLVNillYDTA.....LNVFRD.................HNFDSSSvcvcNADTQ...KIGN....IRGADSGVYV.PLpGAS.....F
 1454- 1609 (112.57/44.45)	NPFPAGGG...QRLlngPAASFGG.LRMI..SAFGG.......SPAS.AS.M..PGAGSGHghgpnggSNSSSCTPP....S.SNPHITGYVDDdpvectcgfsavvnrrlshraglFYEDEVEITG................LADDPGRNKQP.TLLS..............LIQSLCRKN...QIKQA..PG..EPS..SALE......KAGSGG...GAGA.TAG................................................................................................................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     169.57|      52|     163|    2314|    2366|       2
---------------------------------------------------------------------------
 2314- 2366 (88.55/53.68)	PTILSACLVTLEPDAKLRVmPDQFTPDERF.SQISMQNPLSTPQD.VTCTHILVF
 2482- 2535 (81.02/44.57)	PHLEDVCPVFLKTALHLHV.PSIQTADDILnSTNAHQSANDHPLDsILTADVLRF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     130.63|      39|     163|    1676|    1727|       3
---------------------------------------------------------------------------
 1686- 1727 (67.45/71.05)	QARLAFENNMMDFHGNGSSTF......QAFHAPPpalRHKLAGIGG.GR
 2380- 2425 (63.18/36.85)	QNEPQVEDDFLTFEEEGNEDFddadmnDLFWDPP...MDKTSNHGSpGR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      46.86|      14|      16|    1063|    1076|       7
---------------------------------------------------------------------------
 1063- 1076 (23.68/16.21)	NSACITSSNTTIVS
 1080- 1093 (23.18/15.68)	NNSNVTINATTTTS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      35.82|      10|      25|      97|     106|       8
---------------------------------------------------------------------------
   97-  106 (17.48/ 9.87)	LSLAAAKELW
  125-  134 (18.34/10.74)	LRVAANQALW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      53.63|      16|      26|     328|     343|      12
---------------------------------------------------------------------------
  328-  343 (28.93/19.03)	TDLPPV..VEVVSGGHKM
  353-  370 (24.70/15.04)	TDLDDMehMEHMAELQKM
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP05446 with Med13 domain of Kingdom Metazoa
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