<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP05213

Description Mediator of RNA polymerase II transcription subunit 13
SequenceMTTKNHKDIFDDYSNLYHKDSIANDVTLEDCHTNVFPLADITKLKWKCYSTSNRSITKNKEAIKADLVLKAYSLCISENYICLWRRIPSEFPENDYPRGEFTLRTAKELWVFWFDKREPRNLASYTCDLVVREEYCGDYQNEGVNYDIRCLLFRALHNLMERSLFTMGQKRFGKWFVATGSKEELKQSMPQRNYSLAYGFSFFVHGDNMICATINIQRQPLMCKIGLLDYFSKKTIPLILGPHGIRATLVLDQIECVKEETIQITNKHKRNILVAKQYKEVETIAEEQYNDWRGFYPLPVYDAEDVKYRVPSVTEILPRMTLVEIDNLKMLWPTECIGIIMDEDESNGDRKCFYEKIVDEDQEVATIMSNNKENPDVKIEAINNPVIEDVERVNTETYCRHRIEDIDKNSRIFEDIIVPSEYAVLKENIIDKPMLNMPLSSTENNDTKDKFESAISFLMDIDNDNKKDESKPMEIDDDDDIESFLKFDNSVKPPLSKSPEKNTNENKSDKMDTNEKKNDNDSKQKDKVDRNEETKDSTMNVPKYVEPAIGRKWRFSNYCSKTNCVCHLCRKFQKIFPSHSNKIPSKNDSKGSALRDELMTIDEEKENEPIQGNKSDVKQMKRDDIKSYDALVEQRKLAKTVIPIHPNRSKLITQQQNIRNHKSYKTPNYKLRDHVKDFQTMDFGNPKLFSDYRLLSGYNKTQGITSKESFPKFAPCAKTKFEYHRKKKYILSKISHKKCKKKVYHLSNPMAKVFSTPAVTKRNRLINETRQRYTFPKEDTLMARDVTFYDRSVQKETNGMLNSFISCLQDIKYNRKNEYKFKLYSDYEIIRIKSNKKFKNCFDDEERLKLYDFSNEEKKMEHYEIVNDHILRNLGKMYLTDDSNTTDLKYLDPFMWNTEEINNFNVSSLIKDNEPRIYNIPEYIVMSNFLYIVEDRRISRSLRRKDSKFHQRRMFPLRFDDGFSWDNSIICDEPIGTILRDSKERFMENYYSEYLFKPKEEAMLRNEDINKVNCTFDIENSEIPLDVENYFDNLGKKNVMEVNILDDDDLDSFNMDEFLKDATSSNSIDEAIVEEPENDLPDIFNGTDEFDVNDICFKIDPGLIVEKDSLNEIETLFNDSKRKFEQDKLLSHQIYECLGISKSNNSSNNGFKVNEELDTMFEEIKNNPLSHVQVNTEVLFENLEDIFFKNESKASFVDDLGGYNNFFTNINYSKIVPSNLRPKKQYIDDIWKKPKPTWCNQFKQVLDYEIGVYNLPESLIKKNCQNVSFIEILRKILQELDNHRNVVDDISIKISESKIKPFWKNLILNQNVETVYFPMRKFYNDRHKNAILIQEDPFAMVGIEEHLNFHQLLSVIDTYKRKKVYKLTECFEVLREDQISSKNILKRRYDRMLRAFDFSNRFLLARHFDDSDYKHHMMYDYEFSNQSYGMHGIPQHHGYGYDYYNYSQNYNNPIDEFGDSFNSYGMQNQQHNQSYYNDPMHYPGRHNNQMYHNYNTNMHQNAHNHMPYYDNFINDDFDSQIMGHNNMNSWNQGNHMPYGHSRNSYLSNYHQSQSNMYVGNPQSFSRKMPMIMENDEEKACAIRDNETIMKTRYFKSLNDTNMYYKSLSDNYIGCYALLKYNFKQTIYSETNNVTKQNQYHTFNYNKRLELTYLNDSQRITNDISTFKERNLFSKKFESQICIRKKASHISEDELACFNALQHLVNTQSLHSIFIEPHYYNRNLTKYKIKHQISEPMNLFGNSVMQINDNIFGMAGFITHDEMERRKFEFSKRASKNPYNFEPELEQMPPYKIRRECEPDKEESNIKLFPCRVGYSDYTMDSVICGTGNDEDGEWANQLFDSLICGTEEAVPNNDMEIDYGLPNSNSSLENFVDQFNSFAQDIDKYAGVTSNTNKIYNNIYDENENEELLRARFKDCSIDENYSESLVPMPMEELFKQFAGSPEPVVINNTGLSKKESPKTSPKEEKEIDYFSIMTELAYRVKNKAETVDIKEAILTTKVNPSIVDISLYDDDDEEETSVTKYEVPFAQNQFNCRTLYNSYDPSYPIYDESEDMCYTVDDYGNICEGDSNDNNCDLSYDSLDSENSFGSILGEKKEAQIIAEAEDAVYEELFAESSSDEKELDEDCEKINYEEVLPHEHFKSEYCEKYFRKHIFPVVLCRELLNNWTGMNLITKAMLPRYSKINDIYALCMAADSQEMYDNLRDSVIKKIRKLKKNKIAKFAPDYYKIIEKIKDYGYTLPYETYILYREGPALSYAHYLLHNDVDNFIHLPDCECIECAQLPPIPTYIDLDDSTSKNPYIYNYKNPPFIKKGEKLYDISSTARRYVPPMMPHHTESMQRKCALETINSLRHRYKYANIIRSINERNEKTIKNGRYYKCGALAYKNNEMTNRSNATVVIEVDKKIKINVVKGPLPPPYLRRNQCGAPVTRNAYERLVPKSVYLKMRKKRLAPLNFKQPKPVFFKGYDMKCSDRVPLYNEIVSASNPPPPEALVEEIEEEDEYDECYDLNSLSYNEDCPVLENNSDAVNMSLESNCGPGINSHNPLTPNTNPLTPSMNPLTPSNNPLTPSMNPNTPSMNPLTPGNGIGEQSQIINPLTPNANSLASSGPPSNYMNPGSNSYPNPGSNNYPNPGSNSYPNPGSNSYSNPGSNSYSNPASNTYPNPGSNNSVLQSIISQPSSQGNPHNFIGGNISSPSSFHGGNQMNSSMMPSPIISNENQFIGNSGNYNYNRQMNPGSYNPMRDSYNSGMYQNRNNPNVFQGNEKHNIPYPMDGGNNMYEQNQYHSNMSQMSNTDSMSSIHSEMNSNMSQSMNSHYNSPMQQNYNSPNTIPDRNSQHYGMDTMNHGMVGNMNQGYMNSNNHHMMSGNQKGSYNNPMVESQFYNKMPTMNYDNNSQGYINNRNQYSDENNPNEASYDSSYNNMSGHQQMNSNEVFHTKTNSYEQSPGNNYPGNNPYPMNYNQNNYMDSENGNGQMCNNDDFNNCNMDTNSYDMQQNCKDSHASSENSPMPLLYNEAINPTEPSIIDSNTEYNCLYYEMEVDSVPPASSLPPVETEKLTIESHYILPIKPSVKVEEPKKDVISRRDIKKDSIGISLLLQDSVFELYYDIIFDGCPICSCNSNIMSDDYGMYLRPPSNRNSTRLNKPEWNGFYLAGQKSKCQCGFSALRHKLFSYNSGLFKEDAEEGSGLVAVYDQHSNKYHTFYNEPTETDFSFINLLRIQSLNREMGAQISVLQNMLTKCIVPKTNAISNKYLNKCSNYMFSQVDINEKEHAIYNSFKASIEDDGESHLCLPNRNRKLIDYPSFLHPWSIQFPNNAKEASDPDCYIVLDNVSKFFKNIIHNTQCFGSEKRKSYKTLTWSELVRNAYKNTKGPDECPFAPEPIPSVIVASERDVISCAPQIVCSWEKLALGPIDQPKDILYFAITPDNDFIVDKTKLYLYEVSKMYETYKLGRHIKLSKDIVEDGIFRVPVMDSNHYKIETDSEKGYYEIFDCIINDKEYVEKMKSYALFIEKQVYQFFEYNDILFDRRAYWECVKQELSGFPSGSHDTSLGGQHGSHEYVPGNYPPGYYDHSSPPNFNRSGNEMDQQRSDSPIKDEDMADMPHIVVLYVINPFNFGTGVKQAEQPKFALISLMRAFNSFLMKLDSTKRIRIQLEIINLQSILDYAGYISDKKRNERWYAPDSIFTRNKNFKFSAYEQIRSLSFAVYTHSRIYNAEDLRYVLSKSVTKFGYSSTVIDQITEGVKAGEKIFKLPCNLYRLSLPKRNPIQVNGKILKRHVEDTVLFVSYCLIEDKWLAVTVSDHLGYLLDNCLINLQVNQKENELVRYKKGHRILDAISRLWMYVMGVLASETRNWRVVIGRFGRVGHTEFKAWMHILSKTNIKRYNSKIKEACNLCAETFTSQNCPVIVSACFVSMEAENNLRLFHNAVSKEKVMASAAMNTRPTKQKVIHSPDDVSVTHILVFPTTVDLSADLGQGKEDQKDDFDSLELEDGNEDILNLITDELINDSNEKENNSTELENELEGNFFNIDPSDIYASNQPMAIGYYISTAPTGKLPNWFWSQCPKAKDRCPVHLRNSLHIVTSNILQSDEQMLNFKNTDSTHPLDSTATDDVLRYVLKTYNALSWLNVDCVTSERRSCLPIHIQALARLCDAVETFMF
Length4152
PositionMiddle
OrganismParastrongyloides trichosuri (Possum-specific nematode worm)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Nematoda> Chromadorea> Rhabditida> Tylenchina> Panagrolaimomorpha> Strongyloidoidea> Strongyloididae> Parastrongyloides.
Aromaticity0.11
Grand average of hydropathy-0.758
Instability index49.03
Isoelectric point5.50
Molecular weight481932.06
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP05213
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      86.22|      15|      15|    2612|    2626|       1
---------------------------------------------------------------------------
 1483- 1495 (24.93/ 6.69)	PG..RHNNQMYHNY.N
 2613- 2628 (31.74/10.63)	PGSNSYPNPGSNNYpN
 2629- 2644 (29.56/ 9.37)	PGSNSYPNPGSNSYsN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             9|     441.35|      50|      50|    2507|    2556|       2
---------------------------------------------------------------------------
 2449- 2506 (35.40/12.08)	APLNF......KQPKPVFFK.....GydmkcS.....DRVPLYNE.....I...VSAS.....NPP.......PPE.AlveeieeeD.EYD........ECYDL
 2507- 2556 (87.02/43.94)	NSLSY......NEDCPVLEN.....N.....S.....DAVNMSLESNCGPG...INSH.....NPL.......TPN.T........N.PLT........PSMNP
 2557- 2606 (61.17/27.98)	LTPSN......NPLTPSMNP.....N.....T.....PSMN.PLTPGNGIG...EQSQ...iiNPL.......TPN.A........N.SLA........SS.GP
 2646- 2695 (40.28/15.10)	GSNSY.....sNPASNTYPN...pgS.....N.....NSVLQSIISQPSSQ...GNPH.....NFI.......GGNiS........S.P..........SSF..
 2702- 2744 (56.13/24.88)	NS.SM......MPS.PIISN.....E.....N.....QFI.....GNSG.N...YNYN.....RQM.......NPG.S.......yN.PMR........DSYNS
 2778- 2827 (49.21/20.61)	NQYHS......NMSQMSNTD.....S.....M.....SSIHSEMNSNMSQS...MNSH...ynSPM.......QQN.Y........NsPNT.......iP....
 2828- 2886 (26.47/ 6.57)	..................DR.....N.....SqhygmDTMNHGMVGNMNQG..yMNSN.....NHHmmsgnqkGSY.N........N.PMVesqfynkmPTMNY
 2889- 2940 (48.64/20.25)	NSQGY....inNRNQYSDEN.....N.....P.....NEASYDSSYNNMSGhqqMNSN.....EVF.......HTK.T........N.SYE........QS...
 2958- 3018 (37.03/13.09)	NN..YmdsengN.GQMCNNDdfnncN.....M.....DTNSYDMQQNCK......DSHassenSPM.......PLL.Y....neaiN.PTE........PS...
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     188.80|      46|      47|    1145|    1191|       3
---------------------------------------------------------------------------
 1011- 1045 (44.10/25.14)	KVN..........CTFD......IE.....N...SEIPLDVENYFD...NLGK...KN..........vmevNIL
 1046- 1097 (54.39/33.34)	D.D..........DDLDSFnMDEFL.....KDATSSNSIDEAIVEEpenDLPDIF..NG.tdefDVND....ICF
 1098- 1151 (35.78/18.50)	KIDpglivekdslNEIETL.FNDSKrkfeqDKLLSH.QI.....YE...CLG.......isksnNSSN....NGF
 1152- 1191 (54.53/38.85)	KVN..........EELDTM.FEEIK.....NNPLSHVQVNTEVLFE...NLEDIFfKNE................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.75|      13|      18|    1414|    1427|       5
---------------------------------------------------------------------------
 1414- 1427 (22.17/17.12)	KHHmMYDYEFSNQS
 1435- 1447 (27.58/15.64)	QHH.GYGYDYYNYS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      34.73|      10|      19|    3286|    3295|       6
---------------------------------------------------------------------------
 3286- 3295 (17.29/ 8.52)	LPNRNRKLID
 3307- 3316 (17.45/ 8.69)	FPNNAKEASD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     217.75|      48|      52|    2046|    2093|       7
---------------------------------------------------------------------------
 1899- 1924 (24.91/ 6.65)	IYDENENEELLRARFK...........DCS.IdeNYSE..............
 2008- 2044 (49.68/21.18)	LYDDDDEEETSVTKYE..VPFAQN.QFNCR.T..LYNSYDP..SY.......
 2046- 2093 (80.37/39.18)	IYDESEDMCYTVDDYGNICEGDSN.DNNCD.L..SYDSLDSENSFGSILGEK
 2098- 2146 (62.80/28.88)	IIAEAEDAVYE.ELFAESSSDEKElDEDCEkI..NYEEVLPHEHFKSEYCEK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     165.04|      33|      33|    1505|    1537|       8
---------------------------------------------------------------------------
 1505- 1537 (64.84/27.88)	HMPYYDNFI......NDD.......FDSQIMGH.NNMNSWNQGNHMP
 1542- 1561 (32.85/10.48)	RNSYLSNY.................HQSQ.....SNMYV...GN..P
 1567- 1603 (31.90/ 9.96)	KMPM...IM......ENDeekacaiRDNETIMK.TRYFKSLNDTNMY
 1604- 1643 (35.45/11.89)	YKSLSDNYIgcyallKYN.......FKQTIYSEtNNVTKQNQYHTFN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      69.43|      20|      34|     609|     628|       9
---------------------------------------------------------------------------
  609-  628 (36.20/24.16)	PIQGNKSDV..KQMKRDDIKSY
  643-  664 (33.23/21.41)	PIHPNRSKLitQQQNIRNHKSY
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.66|      19|      34|     932|     954|      10
---------------------------------------------------------------------------
  932-  954 (24.42/27.90)	IVEDRRISRSLRrkDSKfhQRRM
  969-  987 (36.23/22.64)	IICDEPIGTILR..DSK..ERFM
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     119.18|      31|      50|    1650|    1680|      11
---------------------------------------------------------------------------
 1650- 1680 (53.37/37.46)	LTYLNDSQRITND...I...STF......KE.....RNL.FSKK.......FESQI
 1694- 1732 (39.68/25.38)	LACFNALQHLVNTqslH...SIF......IEphyynRNL.TKYK.......IKHQI
 1738- 1784 (26.13/13.44)	L.FGNSVMQINDN...IfgmAGFithdemER.....RKFeFSKRasknpynFEPEL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.50|      18|      50|    3191|    3211|      12
---------------------------------------------------------------------------
 3191- 3211 (28.39/21.53)	SNKYhtfYNEPTETDFSFINL
 3244- 3261 (34.11/17.02)	SNKY...LNKCSNYMFSQVDI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.13|      11|      23|    1819|    1829|      14
---------------------------------------------------------------------------
 1819- 1829 (24.00/13.43)	MDSVICGT.....GND
 1839- 1854 (17.13/ 7.31)	FDSLICGTeeavpNND
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      80.97|      18|      18|     811|     828|      15
---------------------------------------------------------------------------
  811-  828 (35.16/18.15)	IK.YNRKNEYKFK.LYSDYE
  832-  846 (21.41/ 7.77)	IK.SNKK....FKnCFDDEE
  848-  864 (24.40/10.03)	LKlYDFSNEEK.K.M.EHYE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      59.34|      17|      18|    1374|    1390|      16
---------------------------------------------------------------------------
 1374- 1390 (28.91/15.12)	LREDQISSKNILKRRYD
 1393- 1409 (30.43/16.31)	LRAFDFSNRFLLARHFD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      56.16|      17|      20|     874|     893|      17
---------------------------------------------------------------------------
  877-  893 (30.43/21.52)	MYLTDDSNTTDLKYL....DP
  895-  915 (25.72/ 8.50)	MWNTEEINNFNVSSLikdnEP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      84.24|      19|      19|    2266|    2284|      18
---------------------------------------------------------------------------
 2266- 2284 (38.83/22.94)	FI....HLPDCECIECAQLPP.I.....P
 2286- 2305 (21.08/ 8.40)	YI....DLDDS....TSKNPY.IynyknP
 2307- 2330 (24.33/11.06)	FIkkgeKLYDISSTARRYVPPmM.....P
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      77.82|      27|      47|    1259|    1287|      19
---------------------------------------------------------------------------
 1259- 1287 (42.94/28.46)	LIK...KN...CQNVSFIEI.LRKILQelDNHRNVV
 1298- 1331 (34.87/17.13)	KIKpfwKNlilNQNVETVYFpMRKFYN..DRHKNAI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.08|      12|      48|    2373|    2384|      20
---------------------------------------------------------------------------
 2373- 2384 (23.87/13.40)	RYYKCGALAYKN
 2418- 2429 (23.21/12.80)	RRNQCGAPVTRN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     129.15|      28|      47|    3353|    3382|      22
---------------------------------------------------------------------------
 3327- 3350 (35.52/15.99)	.SKFFKNIIHNT....QC.FGSEKRKSYKT
 3353- 3382 (46.48/29.88)	WSELVRNAYKNTKgpDECPFAPEPIPSVIV
 3399- 3426 (47.16/23.84)	WEKLALGPIDQPK..DILYFAITPDNDFIV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      63.93|      21|      22|    3450|    3471|      29
---------------------------------------------------------------------------
 3448- 3469 (30.95/26.08)	HIKLSKDiVEDGIFRV..PVMDSN
 3470- 3492 (32.98/22.20)	HYKIETD.SEKGYYEIfdCIINDK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.71|      12|      33|     446|     457|      30
---------------------------------------------------------------------------
  446-  457 (21.24/12.08)	DTKDKFESAISF
  476-  487 (21.47/12.30)	DDDDDIESFLKF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.25|      16|      23|     698|     716|      34
---------------------------------------------------------------------------
  698-  716 (25.88/24.22)	YN.KTQGITSKESFPKfapC
  723-  739 (26.37/14.40)	YHrKKKYILSKISHKK...C
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP05213 with Med13 domain of Kingdom Metazoa
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