<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04954

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMSGINIKTSTLAKSAGSGGHITMPVSILIPSIEEKNKQMQQETLMEKLRAKYRNKPKSYDEIKKSVRMYFIEKHYPMDPLCKATLQTALDKLQHYIKVSSRHGLVERLESLSRQLGLKFMEDQQLLFISTDMFYVEILLDASGNLNDVKVHHECKIEHQSSSSSSCSELLNCLTAGDFADFTVQLEGLSSIYQLNAEPKVKKKAFVALQAMETDILTIYQQQVKKQMAMGISDSYHIMNSSSVGLVLPRRGGHPLKLTYFCPPLHLIEASGKIKTEKGAPATSRDFSIDQVMRSTNGLNATLNLEASSANKLQILPIVSFTSESGEELDLPRCESLNQNNSMLMPATYVLRLSKPIPVCVESLQALGLSGSGNSNEGAKGAGSGSTTVMNLIVQTASGHAIKSTQKGLYVNLPKETHCYFFTDNRRLHGTMLTSLPFTEPAQVAKIIAFLKKQALFYTLLSSCVREQQKQYNDMDSTVILEVTAVSLNQITVELQHPYEESLATIDFLLEDSQPKCTIYCLNNDYEALSDKLTRTVRQVLSIPMVIYKLLKCWDEEHQQKLHGIKGSGPGTGAGSGAGSAIGGGLGASGTSNFRQYSKDSSTGTGGDGSYGASGGFNSVNSMKIESRVRSLADAFAASSSAAAAIAGLISLKQEPVPSVSSRNSDQEIADKYKNIWKDKTPNLKHCVSITPIDGDGKAAPQLPQQQQSSEMQRSGGIEIIPLSAPTNMGNSPGAGSTATTITITPITGSTGGGKEASKEARKIASSGTNSLSSSKRPHDSITSSSSSLNPSSSSGSSSSDSQKEKKRKKKRDDSPMGPPEKIYSRQNSPAAGSITDVSTGTGVARKFSSSSASSSPKAGVTGSSTVGMMGGQSTARPSPKHSPVYSSPKHNSNCNTASNSPKSPFGTQSPKHGSSGKPSMSTLKSATAATSLSPKAEKSSTSTLGVNVGSAGSTPASMSLSISQPSTQPSPSSGPNTSMMRSVPPLANSSNSSSQLSMPMKKDKSISSSSTTGIPGSTSGVVAAAVAALKSSQQQQQQQHQQQKSSVGSLSHLAAGGNLANFAPSAVSAMEINALRKSMGGSGVGAGAGGAVSLMTSTVASTPAIQSTSTTLAAATDLSAPTPAVESGVGVLSALATTASTQDQQQQQQQQQQQQAAGSTSSESSGLGSASSQGVSTEYMVKPSSQEGLKLTINKTGSAGGSISATPHKRESKGASSGSLTSASSGKKQHTGLKPGVNSGPASKKGPSNVSQGSSSSKHLFQKANSSGNLSSKLSSSSSGGGLPLIKSNSTNSFQELGAPRRRPSMSSSGGGAGGNVQRKGSFAAPGSAIGGGNFAASLSGSMSQPPPRFDHHTDMMTILQYASPSMAASMEGFIKGLHNKFQIPKLSQRSSGNNSANDASTTSTSAPSTIQSTTATTTSTIGPSPEEQQQKQLNADLSALAASLTASTRSGGGQQKLSSHSDNDLLLNLSNNAGMGSDDGIDEELLARLAGE
Length1493
PositionMiddle
OrganismDrosophila busckii (Fruit fly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Ephydroidea> Drosophilidae> Drosophila.
Aromaticity0.04
Grand average of hydropathy-0.430
Instability index55.20
Isoelectric point9.34
Molecular weight155417.03
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364059

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP04954
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             8|     822.77|     109|     109|    1020|    1128|       1
---------------------------------------------------------------------------
  555-  647 (83.03/19.40)	..................E.EHQQKLH.GIKG.SGPGtgagSG.AG......S...AIGGGLG.....A..SGTSNF......R..Q.......Y...........SKD..S...........STGT................GGDGSyGAsGGFNSV..........NSMK...IESR.VRSLADAFAASS..S............A..AAAIAG
  653-  756 (87.27/20.74)	QEPVPSVS.....SRNSDQ.EIADKYK.NIWK.DKTP....NL.KHcvsitpI...DGDGKAAPQ.L.PqqQQSSEMqrsggiE..I.......I...........PLSapT...........NMGN................SP.GA.G...........................ST.ATTI.TITPITG..S............T..GGGKEA
  757-  883 (84.73/19.94)	SKEARKIAssgtnSLSSS.....KRPH.DSIT.SSSS....SL.N.......................P..SSSSGS......S..S.......S...........DSQ..KekkrkkkrddsPMGPpekiysrqnspaagsiTDVST.GT.GVARKFSS....SSASSSPK...AGVTgSSTVGMMGGQSTarP............S..PK.HSP
  893- 1020 (102.24/25.47)	NCNTASNS.....P.KSPF.GTQSPKHgSSGK.PSMS....TL.KS......A...TAATSLSPK.AeK..SSTSTL......G..V.......NvgsagstpasmSLS..I...........SQPS................TQPSP.SS.GPNTSMMR........SVPP...LANS.SNS...SSQLSM..PmkkdksisssstTgiPGSTSG
 1024- 1128 (160.99/44.02)	....AAVA.....ALKSSQ.QQQQQQH.QQQK.SSVG....SL.SH......L...AAGGNLANF.A.P..SAVSAM......E..I.......N...........ALR..K...........SMGG................SGVGA.GA.GGAVSLMT....STVASTPA...IQST.STTLAAATDLSA..P............T..PAVESG
 1130- 1240 (110.18/27.98)	GVLSA.LA.....TTASTQdQQQQQQQ.QQQQ.QAAG....ST.SS......E...SSG..LGSA.S.S..QGVST.......E..YmvkpssqE...........GLK..L...........TINK................TG....SA.GGSISATPhkreSKGASSGS...LTSA.SSGKKQHTGLK...................PGVNSG
 1244- 1331 (91.16/21.97)	KKGPSNV..........SQ.GSSSSKH.LFQKaNSSG....NLsSK......LsssSSGGGLPLIkS.N..STNSFQ......E..L.......G...........APR..R...........RPSM................SSSGG.GA.GGNVQRKG....SFAAPGSA...I.......................................G
 1333- 1431 (103.16/25.76)	GNFAAS........LSGSM.SQPPPRF.DHHT.DMMT....IL....................QY.AsP..SMAASM......EgfI.......K...........GLH..N...........KFQI................PKLSQ.RS.SGNNSAND....ASTTSTSApstIQST.T...ATTTSTIG..P............S..PEEQQQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     129.53|      34|     290|       1|      45|       2
---------------------------------------------------------------------------
    1-   34 (58.53/23.30)	MSGINIKTSTLAKSAGSGGHI.TMP.VSILIPSIEE
  292-  327 (44.04/14.38)	MRSTNGLNATLNLEASSANKLqILPiVSFTSESGEE
  368-  389 (26.96/10.06)	LSG.SGNSNEGAKGAGSGSTT.VM............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     130.77|      45|     241|     197|     245|       3
---------------------------------------------------------------------------
  197-  245 (68.96/65.25)	EP.KVKK.KAFVALQAMETDILT..IYQQQvKKQMAMgisDSYHIMNSSSVGL
  439-  487 (61.81/44.86)	EPaQVAKiIAFLKKQALFYTLLSscVREQQ.KQYNDM...DSTVILEVTAVSL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04954 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AAVAALKSSQQQQQQQHQQQKSSVGSLSHLAA
2) HNKFQIPKLSQRSSGNNSANDASTTSTSAPSTIQSTTATTTSTIGPSPEEQQQKQLNADLSALAASLTASTRSGGGQQKLSSHSDNDLLLNLSNNAGMGSDDGIDEELLARLAGE
3) IQSTSTTLAAATDLSAPTPAVESGVGVLSALATTASTQDQQQQQQQQQQQQAAGSTSSESSGLGSASSQGVSTEYMVKPSSQEGLKLTINKTGSAGGSISATPHKRESKGASSGSLTSASSGKKQHTGLKPGVNSGPASKKGPSNVSQGSSSSKHLFQKANSSGNLSSKLSSSSSGGGLPLIKSNSTNSFQELGAPRRRPSMSSSGGGAGGNVQRKGSFAAPGSAIG
4) KHCVSITPIDGDGKAAPQLPQQQQSSEMQRSGGIEIIPLSAPTNMGNSPGAGSTATTITITPITGSTGGGKEASKEARKIASSGTNSLSSSKRPHDSITSSSSSLNPSSSSGSSSSDSQKEKKRKKKRDDSPMGPPEKIYSRQNSPAAGSITDVSTGTGVARKFSSSSASSSPKAGVTGSSTVGMMGGQSTARPSPKHSPVYSSPKHNSNCNTASNSPKSPFGTQSPKHGSSGKPSMSTLKSATAATSLSPKAEKSSTSTLGVNVGSAGSTPASMSLSISQPSTQPSPSSGPNTSMMRSVPPLANSSNSSSQLSMPMKKDKSISSSSTTGIP
5) NFAASLSGSMSQPPPRFDHHTDMMTILQYA
1024
1379
1105
684
1334
1055
1493
1331
1015
1363










Molecular Recognition Features






MoRF SequenceStartStop




1) DGIDEELLARLAGE
2) EKIYSR
1480
820
1493
825