<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04656

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMSGRENAECAPAAGPGAGRERINPLLTSNCHPYPHNNTSPASSSSSSSHQQLAKKIKSECSSQKPIQFDQETLNQIDRVELPRLWGPHLVPLQFVIQRTIAQVFHELQILSHNSQNLEDHDRKKRVVDFVVHSRRQLIKLLVLVKWSCNSVPVHKIMSIVGFLQRQNSQFERNVNILKASKENFHSARMRNYDLPTALQVLTNGYPTLPGRLIDQFDSKPKLSDSEVIKIMQDLNELIRYRLASCEVIPKDFRSYRIYDGRVVFRIDRMFECSLTLGGGELDSQWYLLHVEFLFKVTGPRGKDFMRIPQGPIKDQIIECGNRTMAPNTLPTESDPDPKPPERPIMKLFAYLRHKLKTNSGDLCLNYQLEALHYQASQLGRSAWATHTRLSISADRRNLTVMYWLHARPVSIPVIRPGQQPPKTLPQPTPQGTLTIRRQTERHSPSRTRVLDFLNGYPLKNKLLPGGQDGDFGASYTERLQVVWRPIEVSSQGGSLTQQALADLKIRQGDEVVFTKTEAGLMELELTMNELCLEGIIQRAVRIHTDCTLRRAFSDLTRLERPANGPSTRVVPYFASVRLLAWEITCSSERFAKSIEVVLHARHAVEVFIDQFSGRFRLQSTTHPSRTNEGDKHSAHRLGVGCFSGYNPHRLSQSANKINLNPSGLRDVLVRLKSDILLDQIEFKARMLGIRTTRNLPIQPQLLSLPSYLSSPSTIPQHIFQLTLYCRLSNFPHHYLLIMVTENGIRCALIIVNPNRGDQPTQQAGGTSCWNVHSVLAIEVPTFADDLDEDGGVLDQLSPLPLISCAQFTLSRTQLKYVYHYFTLNQLKNGLRRNGISYEQSLPLTPEIIKRQCFNHRNNRSTLTSSAALPSIKLPTLHPSYEVQGLLGIPLPGFLQPSFFRHHHALKGIFDGHNLAVYVFPHPDSHFGVKVDDDDDDDEDEEPLSRRIQTTRLKPASPPNANNAAVEERKEESSVEENLDGELIDSDEWSIDAQSHQFIMRFQNLEERVDQIYKLSLQKFFNLIRSPASADP
Length1031
PositionTail
OrganismPuccinia sorghi
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Pucciniomycotina> Pucciniomycetes> Pucciniales> Pucciniaceae> Puccinia.
Aromaticity0.07
Grand average of hydropathy-0.435
Instability index50.19
Isoelectric point8.77
Molecular weight116977.87
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP04656
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     245.39|      65|      89|     326|     391|       2
---------------------------------------------------------------------------
  326-  391 (113.38/71.55)	PNTLPTESDPDPKPPERPIMKlFAYLRHK.LKTNSGDLCLNYQLEALHYQASQLG...RSAWATHT.RLSI
  416-  481 (103.79/61.21)	PGQQPPKTLPQPTPQGTLTIR.RQTERHSpSRTRVLDFLNGYPLKNKLLPGGQDG...DFG.ASYTeRLQV
  622-  651 (28.21/10.52)	...........................HP.SRTNEGD..........KHSAHRLGvgcFSGYNPH..RLS.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      86.64|      29|     530|      11|      64|       3
---------------------------------------------------------------------------
   11-   64 (31.50/58.58)	PAAGPGAgreRINPLLtsnchpyphnntspasssssSSHQQLAKKIksECSSQK
  561-  589 (55.13/28.08)	PANGPST...RVVPYF....................ASVRLLAWEI..TCSSER
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     194.72|      61|     530|     259|     322|       4
---------------------------------------------------------------------------
  259-  322 (104.55/78.16)	DGRVVFRID..RMFECS.LTLGGGELDsqwYLLHVEFLFKVTGPRGKDFMRIPQG.PIKDQII..ECGNR
  789-  855 (90.16/58.46)	DGGVLDQLSplPLISCAqFTLSRTQLK...YVYHYFTLNQLKNGLRRNGISYEQSlPLTPEIIkrQCFNH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     131.54|      40|     728|     150|     195|       6
---------------------------------------------------------------------------
  150-  195 (61.16/62.39)	SVPVHKIMSI..VGFLQrqnSQFERNVNILKASkenFHSARMRNYDLP
  879-  920 (70.38/52.06)	SYEVQGLLGIplPGFLQ...PSFFRHHHALKGI...FDGHNLAVYVFP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04656 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) GVKVDDDDDDDEDEEPLSRRIQTTRLKPASPPNANNAAVEERKEESSVEENL
2) MSGRENAECAPAAGPGAGRERINPLLTSNCHPYPHNNTSPASSSSSSSHQQLAKKI
927
1
978
56










Molecular Recognition Features






MoRF SequenceStartStop




1) QLAKKIK
51
57