<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04613

Description Mediator of RNA polymerase II transcription subunit 5
SequenceMERFISLTRASCRRGVEGTEWAEQITKIQQQEEEEEDYGSALIELTLSSKVTSIRLLSYYRTAISKQIIEPSRAASKLIITSNRITTNKLNWLAEPLLSQQNLAVSVEEHLDPLLELIDSTTTTKEGEEQPISVLAQILLRSIANELLAKPVTSKLDNRTILKITQTIAPNDDHLHSLLRHLSPSTAKGLSYNPPSSTSTTASGLIAYILLTPGGATSFQPFISFALSRRDSFILELLSAAIQLGTVPKQELEEEDQDINLIPWIALLAGGRLAEIIRTVIDSKIIIDIELLDTHQLQLLSSKINPQKIWYCLLDSLIVNNLVDSDTLPHLVSLSDSVGTDESVPVYYKYAHHSCLELGLHSLANSLSVDISSTTISELINKLVQSLRLACESFDLSRCISITKSITKSESLSVLFIWAKPVQILAPIRELLDDWNSIRNRTSNNHHLKNDLEDHRTVHSNENDGGEHHHHQNSVIDESSSGSEFEKFGALLGWIQGVIGRFNLMANLGAHLGSTKGFTINYTSNPSQSYSLTDLGPIQMTTLSTWIESLYGSTGIPDDLLIKTDPRIFFMISTSLFKQSFDALKAGLIDLQTFRDGLSYFEHKLLVEGCAIGVIGWLLDELSRLGPISPSSFPTALIEILQSILLSESISSTALFLVSARSLKVLRQFDKLFTDRKTCLIEEDSNSDLNRRVQLNLEVIEQRLTVLPTTEMITPARFISFDFGESNVSWSQALDMAVKMAIDGNLSEEEDSQDRTIPIWSLLPQIMKALKAKEFVKILIGSIVKAIKPDPDPPSPKPCRRVEKAWDIHQYQRLERAVGLAVDILTWPADLPSSESRRLRVLEELFGGWGNRLSKTTDIDHAEIERDILLGCLDRLHALELVHSNSSGVSSSLNQDTDGNSTGDVEMRTSDDHHHTGNQTNDDDKDLVRNLLSLRFRLISMESLRREKLKQIGVLDVYLDAQRQSLGDCERDGERQDHQGMRSVDDLGRGLEGVGQGERQNGDQVLIHSHGQLLVEKADHQLNDLGVEDVNGLENQNRIEIELEPEPATGAQTATATATATGSETQTRAATEIGSDVVEHTENDKEHEKESDLDKVEEEVEVEVGDKEEEKGELEDKAGLDVKGEQDEKEKEGLEEKEKEEEEQEQEGKEKEKGEQEQEEKEKEEEKEMEMEKGEQEEKEESKEKSRSESGIDNEIEQVEDKMEVDPKESVESTKGESTIPDTKKVHFLEPARSSTEPEKEMCTIKEDDNHQASIRDEPVRKKIKKSRTHIQVDHQLVELTVLDWIISSLTISPVSGLSDPSTSTSSTTHSSLDSSDLIEFRKIFLPACFPVHPLDKFKLVGLVDL
Length1346
PositionTail
OrganismPuccinia striiformis f. sp. tritici PST-78
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Basidiomycota> Pucciniomycotina> Pucciniomycetes> Pucciniales> Pucciniaceae> Puccinia.
Aromaticity0.05
Grand average of hydropathy-0.443
Instability index48.03
Isoelectric point4.80
Molecular weight150456.05
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364142

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP04613
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|      90.84|      19|      19|    1125|    1143|       1
---------------------------------------------------------------------------
 1122- 1141 (28.65/12.27)	VK......gEQDEKEKEGLEEKEKEE
 1155- 1171 (22.50/ 8.15)	.........EQEQEEKEKEEEKEMEM
 1172- 1187 (20.03/ 6.49)	EK......gEQEEKE....ESKEKSR
 1188- 1212 (19.67/ 6.26)	SEsgidneiEQVE.DKMEVDPKESVE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     197.04|      53|      61|     869|     929|       2
---------------------------------------------------------------------------
  448-  478 (35.60/15.10)	........................LKN........DLEDHRTVHSNENDGGEHHHHQNSVIDE......
  869-  929 (81.41/68.47)	LLGCLDRLHALElvhsnsSGVSSSLNQ........DTDGNSTGDVEMRTSDDHHHTGNQTNDDdkDLVR
  931-  989 (80.04/49.21)	LLSLRFRLISME......SLRREKLKQigvldvylDAQRQSLGDCE.RDGERQDHQGMRSVD...DLGR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     124.82|      39|      59|     205|     243|       3
---------------------------------------------------------------------------
  205-  243 (65.67/39.95)	LIAYILLTPGG......ATSFQPFISFALSRRDSFILELLSAAIQ
  261-  305 (59.15/35.27)	LIPWIALLAGGrlaeiiRTVIDSKIIIDIELLDTHQLQLLSSKIN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     163.20|      48|      49|     494|     542|       4
---------------------------------------------------------------------------
  494-  542 (80.58/59.41)	WIQGVIGRFNLMANLGAHLGSTKGFTINyTSNPSQSYSLTDLGPIQMTT
  546-  593 (82.62/55.60)	WIESLYGSTGIPDDLLIKTDPRIFFMIS.TSLFKQSFDALKAGLIDLQT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      77.54|      23|     182|     175|     198|       5
---------------------------------------------------------------------------
  175-  198 (36.72/29.40)	LHSLLRHLSPSTAKGLSyNPPSST
 1283- 1305 (40.82/26.92)	LDWIISSLTISPVSGLS.DPSTST
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.97|      17|      76|      76|      96|       9
---------------------------------------------------------------------------
   76-   96 (22.37/26.85)	SKLiitSNRiTTNKLNWLAEP
  154-  170 (27.60/17.24)	SKL...DNR.TILKITQTIAP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      81.37|      30|      59|    1019|    1050|      11
---------------------------------------------------------------------------
 1019- 1050 (45.79/35.60)	DHQLNDLGVEDVNGLENQ.........NRIEIELEPEpaTG
 1067- 1105 (35.57/20.36)	TRAATEIGSDVVEHTENDkehekesdlDKVEEEVEVE..VG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     115.59|      36|      40|     780|     816|      12
---------------------------------------------------------------------------
  780-  816 (63.00/47.87)	IGSIVKAIKPDPDPPSPKPCR.............RVEKAWDIhQYQRLER
  818-  866 (52.59/34.01)	VGLAVDILTWPADLPSSESRRlrvleelfggwgnRLSKTTDI.DHAEIER
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04613 with Med5 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DLGVEDVNGLENQNRIEIELEPEPATGAQTATATATATGSETQTRAATEIGSDVVEHTENDKEHEKESDLDKVEEEVEVEVGDKEEEKGELEDKAGLDVKGEQDEKEKEGLEEKEKEEEEQEQEGKEKEKGEQEQEEKEKEEEKEMEMEKGEQEEKEESKEKSRSESGIDNEIEQVEDKMEVDPKESVESTKGESTIPDTKKVHFLEPARSSTEPEKEMCTIKEDDNHQASIRDEPVRKKIKKSR
2) SGVSSSLNQDTDGNSTGDVEMRTSDDHHHTGNQTNDDD
3) SLGDCERDGERQDHQGMRSVDDLGRGLEGVGQGERQNGDQVLIHSHGQL
4) SNNHHLKNDLEDHRTVHSNENDGGEHHHHQNSVI
1024
887
965
443
1268
924
1013
476










Molecular Recognition Features






MoRF SequenceStartStop




NANANA