<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04577

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMPHVKQSNVIGSSSAVGTLRAADPQKMTQSLLRPFPSAGLGSKHQQPLPHLYPSIMSDHEPGPAVDRVTSMAASISTDPRASIPSPARSNIPTTLPAAAAATTSEAPPELPPLWMNAVSIKELAGRMIQSGYAKLQELTRSLPNAPEMERKRQLLDYALSQRKEFQKLLVLTKWGRQHKDWQKMMNLHRFIDEQNDAYRRTADGLYFMVDKFKRMAETPVPDVPTAIDVLGTGRYQRLPKVLRGVLPRKPLTKVQTRETLVTLDELIKRRLELEIIPDSMRKNLVIEKGCVTFNVNLFKVSLTLVGKDFSLPWRIVQLETHVKSADSYGGVCPGFMDHQMQYILNSAQVLIEQVQKRKAKPPLSGVVEQPKPTSISASKLPTVTRNTARKPTSTSASSVAAQKPTIPASSSSNSKDRGKKEKSKDKAKVTMAKDTVDVKGKGNGPRESKEKGKSTATMKVRTMKKDKGQLPATAPSTSEAGEDTKTRGTTTAPITSTTPIPTTTSSAQPAEATSVHPAHSSAPKPHNPYAILHLHDFLQKLTLRLQLQMLYMQAIFLSRERWQGALKIEYQQDKEMLRIHFWDHEIPGPDPKTVPAGFKVPHRYNVLEMGIKVEEPLKDPMFETWVRKRKRKFWKHVNTDLVSATVFGDKSRPRRYFDIRCYSVIPDLKGNVQKLELVNPQTEQKIPLTLDPSNLDLERLLNNIAELQCRFTLRRMRNIALGLPINSGLAEIGFITPPLAEGDNAASSDLSSIPGDSLPLSPGRSTGSASSVYKRKRDADDVEASRTMKKGKQDAEGVEAAVNKRKRELEMGHEPERTSGAQDGRMLFQEQDLELKEWFPTGTAHECIQPELEVTYRPQRTLKIVVDIRTGRIMVLMDGKRGQTTGDIKLMGIEQRLNDHIEETLDILMYMRYSSYIDDIEAWARRLGLRAFRNTPLKSEEDSKIAKLLPLHRIYLRLPAFSEGWIVVAVAPLKQWLTTYGDRPAGSEAKARLNLGVKEDDAIFRVWFMMTKYERRRVKRFGRVKKEMLREIEFITPLSVDDVFGREFSGYQGTKPSQMIVNADDGEGSFPRKKIKSMESSIPVKGQAAEDGSSSAELESLDEMLHSWDHLDYETLAKVETICRLQYGFTRVTTELHMRQVPYEYILGSSLPRVTPEELEIPETRIAGRGMRICIPVDYFAEEDAGWRSAILPFGELYISIESNVVRGATSGVTKVPSAKIPFGTPTTVKSAHVLSVCVIKMQLGVPPIEKPASEDFTFEPQSHAITFRFVDVDEIEDCVALLLARMRSIVMMTRLAQQTVRNKKRLEKIGVQFERFDLVTLSLTFGEDVQIKLWWEDGGEEKLPDGSIVAKDGTFRTAFIQQKSINATGTKGIPLEMYERIKDFFEEHLNTGRDLAQSLLMLWHMAPLLRLVWQVEQKRNVDAYALESGKPLVKLTVHSLSRVQLVYAEIYSVDFVLHRSGTFAVYDSARSSPKSKDAMQVYSEIPQFYAGAGLTTVLANSVYDASVPAGTRALAVPYGVFFDGPMLELAVTKVDSHLTNITSLMWFNSEAQKRLPATDVKFDIKTETESSVIIRTEARAFYISVNAASKWKVATLGMPGGEVSGENIRNDLWRMMSDSISAKLNALPPGTNMRPFLMTFVDMLLMWRDPMADLLKLIEFEKKQADENLRLTVEWCLAIPPDAPEYLGKVGTPAFHVQLDMHRMSVLYRFTDTQTNENVLFPLLYNVTTGVLCPWEATPDNDFTQLSEMNNLLYQRLRDDWNKENFLSLLVKMSLTPKIAESMGLPGKLFPIIKQCCKGNLRYLKKK
Length1808
PositionTail
OrganismSpizellomyces punctatus (strain DAOM BR117)
KingdomFungi
LineageEukaryota> Fungi> Fungi incertae sedis> Chytridiomycota> Chytridiomycota incertae sedis> Chytridiomycetes> Spizellomycetales> Spizellomycetaceae> Spizellomyces.
Aromaticity0.07
Grand average of hydropathy-0.381
Instability index42.85
Isoelectric point9.17
Molecular weight203458.12
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP04577
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     231.70|      79|     324|    1116|    1218|       1
---------------------------------------------------------------------------
  808-  850 (45.37/12.54)	ELEMGHEP.ERTSGAQDGRMLFQEqdLELKEWFPTGTAHE.CIqP.................................................
 1135- 1218 (124.08/106.08)	ELHMRQVPyEYILGSSLPRVTPEE..LEIPETRIAGRGMRiCI.P.....vdYFAEEDAGWRSAILPFGELYIS..IESNVVRgATSGVTKVPS
 1485- 1544 (62.25/25.66)	...........................EIPQF.YAGAGLT.....tvlansvYDASVPAGTRALAVPYGVFFDGpmLELAVTK.VDSHLTNITS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.16|      14|      15|     473|     486|       2
---------------------------------------------------------------------------
  473-  486 (24.85/13.26)	TAPSTSEAGEDTKT
  491-  504 (26.31/14.53)	TAPITSTTPIPTTT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     230.93|      79|     281|     936|    1086|       3
---------------------------------------------------------------------------
 1000- 1086 (120.33/188.72)	DDAI......FRVWFMMTKYERRRVKRFGRVKK..EMLREIEFITpLSVddVFGREF.......SGYQGTKPSQMIVNADdgeGSFPRKKIKsmESSIPVKG
 1277- 1370 (110.60/49.50)	EDCValllarMRSIVMMTRLAQQTVRNKKRLEKigVQFERFDLVT.LSL..TFGEDVqiklwweDGGEEKLPDGSIVAKD...GTFRTAFIQ..QKSINATG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     159.17|      50|     322|      33|      90|       4
---------------------------------------------------------------------------
   36-   90 (86.10/70.13)	PSA..GLGSKHQQPLPHLYPSIMSDHEPGPavdrvTSMAASISTDPRASIPSPARSN
  224-  250 (27.53/ 6.26)	PTAidVLGTGRYQRLPKVLRGVL...PRKP...........................
  381-  413 (45.54/23.87)	...................PTVTRNTARKP.....TSTSASSVAAQKPTIPASSSSN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     127.22|      39|     322|     418|     467|       5
---------------------------------------------------------------------------
  418-  456 (62.73/35.84)	GKKEKSKDKAKVTMAKDTVDVK.GKGNGPRESKEKGKSTA
  742-  779 (54.19/28.84)	GDNAASSDLS..SIPGDSLPLSpGRSTGSASSVYKRKRDA
  791-  806 (10.29/12.40)	GKQDAEGVEAAVNKRK........................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     126.61|      42|      49|    1665|    1713|       6
---------------------------------------------------------------------------
 1665- 1713 (63.09/63.85)	QADENLRLTVEWCLAIPPDAPEYlgkvGTP..AFhVQL.DMHrmSVLY.RFTD
 1715- 1760 (63.53/41.82)	QTNENVLFPLLYNVTTGVLCPWE....ATPdnDF.TQLsEMN..NLLYqRLRD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     108.85|      32|     323|     307|     378|       7
---------------------------------------------------------------------------
  190-  223 (52.65/13.36)	FIDEQNDAYRRTADGLYFMVDkfKRMAETPVPDV
  335-  366 (56.19/14.54)	FMDHQMQYILNSAQVLIEQVQ..KRKAKPPLSGV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.46|      15|     387|     595|     612|       8
---------------------------------------------------------------------------
  595-  612 (25.27/25.26)	PAGFKVPHRYNvleMGIK
  984-  998 (26.18/16.72)	PAGSEAKARLN...LGVK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      79.13|      28|     327|     549|     579|      11
---------------------------------------------------------------------------
  549-  579 (45.34/38.56)	MLYMQAiflSRERWQGALK...IEYQ.QD..KEMLRI
  874-  907 (33.79/20.06)	MVLMDG...KRGQTTGDIKlmgIEQRlNDhiEETLDI
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04577 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AKPPLSGVVEQPKPTSISASKLPTVTRNTARKPTSTSASSVAAQKPTIPASSSSNSKDRGKKEKSKDKAKVTMAKDTVDVKGKGNGPRESKEKGKSTATMKVRTMKKDKGQLPATAPSTSEAGEDTKTRGTTTAPITSTTPIPTTTSSAQPAEATSVHPAHSSAPKPHNPY
2) DNAASSDLSSIPGDSLPLSPGRSTGSASSVYKRKRDADDVEASRTMKKGKQDAEGVEAAVNKRKRELEMGHEPERTSGAQDGRM
3) MPHVKQSNVIGSSSAVGTLRAADPQKMTQSLLRPFPSAGLGSKHQQPLPHLYPSIMSDHEPGPAVDRVTSMAASISTDPRASIPSPARSNIPTTLPAAAAATTSEAPP
359
743
1
529
826
108










Molecular Recognition Features






MoRF SequenceStartStop




1) LRYLK
1802
1806