<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04553

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMAPAPQPLEQGLAGYLPQGQEGSPRMNTISLAVLIDFIIQRTYHDLTVLAELLPRKSDMERKIEIYNFSARTRQLFIRLIALVKWANSVSKVDKSANIMSFLDKQNMLFVETADMLARMSRETLVRARLPNFHIPAAVEVLTTGTYNRLPTCIRERIVPPDPITPQEKKQTLLRLNQVIQHRLVTGKLMPQMREFKIRNGRVTFEIKHEFNVSLTVMGDSPNVPWRLLDIDILVEDKETGDGKALVHPLQVNYIHQLIQARLVENPNALSEVYNCLHYFCQSLQLEVLYTQTLRLSYERLDDNIQVEEYIPGVKVSVSYWRELTSKDPKSELGYRLTVQSDPNEIGRPLAVIHVPSLGAKESAEVADRAVRSDHLSMERLIVHTVYIRSVSRLSDLKLEFQAFLKDVDFKLEGTPAILTVPVLTPCLRAEQIHITIDTHTGMLRCHVPKHLDCPIIPEMQTALNNDRTKLPQIMSELRYWITHRRCEKTLQHLPATATESLPFLTVPDNPLLQSGRHKIFVKLHRHPSVILVVQLKEKSNMPNEMEYTFHLGFVAYQTNENDPLPEESSQQLVPVQAPPPNLQPGSTPNAEVPKVYTKLLKLIEFDTFVATHGPGTDVDDTPPHKRKLGNEHGAPSAKQAKTIYPAYFIPELAHVVAMCDEKIPFLNLAQALTKYNIPHSGLQVESNATSLVLKILTLPMPGQTHGEEQKPQSHVLNMPVIEPHIWKDLTKRLLSISIRSQMNKNNQIRVWVVEFVFFSTPLQSTHPKEQGNRRTVYLTYDQINHDFSKTVEDLLKDWSKIVYLYTLVYNFSEHMKNKRLNISDTITINSYNYINLLLGYGPKKEVICNIYWSAQSRGFRLIFMGGITAVNAHSMMKDQLATHLNSQHSLSQMVYILHETYYPLSSIAKLPIIPHLGIPRPQVPVLSFCILPQSPCLIRLAYQAMFCLEIRFRAGRLVSIRDGAYSRFDRNLIEDFTPIQGLKGFLSKYVDENAVYRGRSQSDDDNPPSPLGMEDNFGGPGSVTGSGTGGSSPFLSAGMRGPQSPRDSGLRFPAPHTPPLSSNPHTPASPHPSAGGGSSTSGGNSQNHPNYNLTSPPGPHMPHPSPGGLMPSSPLNPQPSPHMVHSPGPNTLYMQGHQDSPFAAMSPANSNWPGSPSMPRPSPRPGQSPDHKSSQSGNTASNAPSNSNVNAQGMNRMPQANRSWAGAVPTILTHEKLDLLCRPSPHPNKDIGVTPDISPLERFLGCVYIRRQLQRHVQNDETLTVLNSNEPGVVPFKVDNLQCQVILNQVHMQSLHLKITQLPPPPTPDGKQPFQLSPDDLIVLEQFFDTRVAAPPYRPNSLQGFCKVLSCAPQVLKDFIQIMRLEMKPDLGGDQLKWTVQFCMRVPPSAAPIIPIGSPGVLIVRLKILFFLQITRIPYNGKEWKDSPSLVLPMVYDLSSNLTQLAEKREQVQSPAAVAASTLLRRFSEYGVQHGQCSLFPAVRDLLTNLQLPNDVPPPNQAIGPPVGPMVGSSPNPIMHSPMQQMGGPVGPPQVGPGYAQIPQNPGPQ
Length1549
PositionTail
OrganismLucilia cuprina (Green bottle fly) (Australian sheep blowfly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Oestroidea> Calliphoridae> Luciliinae> Lucilia.
Aromaticity0.07
Grand average of hydropathy-0.321
Instability index51.78
Isoelectric point8.72
Molecular weight172988.89
Publications
PubMed=26108605

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP04553
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             6|     225.00|      29|      29|    1070|    1098|       1
---------------------------------------------------------------------------
 1031- 1077 (35.24/11.47)	SSpflSAG..MRGPQ...SPRdsglrfpaphtpplsSNPH.TP.....AS....P...HPSAGGG
 1078- 1109 (50.21/19.79)	SS...TSG..GNSQN...HPN...............YNLT.SP.....PG....PhmpHPSPGGL
 1110- 1128 (31.00/ 9.11)	MP...SSP..LNPQ..................................PS....P...HMVHSPG
 1129- 1166 (36.28/12.05)	PN...TLY..MQGHQ...DSP...............FAAM.SPansnwPGspsmP...RPSPRPG
 1204- 1232 (37.83/12.91)	WA...GAV..PTILT...HEK...............LDLL.CR.....PS....P...HPNKDIG
 1505- 1538 (34.44/11.02)	PP...VGPmvGSSPNpimHSP...............MQQMgGP.....VG....P....PQVGPG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     150.89|      43|     478|     907|     950|       3
---------------------------------------------------------------------------
  907-  950 (77.28/48.40)	IAKLPIIPHLGIPRPQVPVlSFCI.LPQSPCLIRLAYQAMFCLEI
 1362- 1390 (38.35/17.78)	IMRLEMKPDLGGDQLKWTV.QFCMrVPPSA...............
 1392- 1414 (35.27/15.69)	....PIIP.IG.................SPGVLIVRLKILFFLQI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.20|      20|     478|      44|      68|       4
---------------------------------------------------------------------------
   12-   31 (34.37/17.46)	LAGYLPQGQEGSPRMNTISL
   49-   68 (32.82/11.92)	LAELLPRKSDMERKIEIYNF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      74.28|      23|      40|     808|     831|       5
---------------------------------------------------------------------------
  808-  831 (36.11/29.94)	VYnFSEHMKNKRLNISDTIT.INSY
  850-  873 (38.17/26.15)	IY.WSAQSRGFRLIFMGGITaVNAH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.07|      11|      28|    1304|    1314|       6
---------------------------------------------------------------------------
 1304- 1314 (24.57/10.62)	PPPTPDGKQPF
 1335- 1345 (23.50/ 9.81)	PPYRPNSLQGF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     115.86|      37|     389|     581|     635|       8
---------------------------------------------------------------------------
  581-  624 (58.18/66.31)	NLQPGStpNAEvpkvyTKLLKLIEFDTFVATHG....PGTDVDDTP...PH
  681-  724 (57.68/27.14)	GLQVES..NAT.....SLVLKILTLPMPGQTHGeeqkPQSHVLNMPviePH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     145.53|      69|     389|     320|     406|       9
---------------------------------------------------------------------------
  320-  354 (28.95/17.36)	...................................................................................................................................................................................................................................................................................................................................................................................WRELTskdpKSELGYRLTVQSDPNEIGRPLAVIHV
  355-  406 (34.91/38.26)	PSLGAKESAEVADRavrsdhlsmerlivHTVYIRSVSRLSDLKLEFQAFLKD..................................................................................................................................................................................................................................................................................................................................................................
  726-  756 (32.53/10.76)	....................................................vdfklegtpailtvpvltpclraeqihitidthtgmlrchvpkhldcpiipemqtalnndrtklpqimselrywithrrcektlqhlpatateslpfltvpdnpllqsgrhkifvklhrhpsvilvvqlkeksnmpnemeytfhlgfvayqtnendplpeessqqlvpvqapppnlqpgstpnaevpkvytkllkliefdtfvathgpgtdvddtpphkrklgnehgapsakqaktiypayfipelahvvamcdekipflnlaqaltkyniphsglqvesnatslvlkiltlpmpgqthgeeqkpqshvlnmpviephiWKDLT....KRLLSISIRSQMNKNNQIRVWVVEFV
  760-  797 (49.15/24.73)	TPLQSTHPKEQGNR..............RTVYLTYDQINHDFSKTVEDLLKD..................................................................................................................................................................................................................................................................................................................................................................
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.75|      14|      23|     145|     158|      10
---------------------------------------------------------------------------
  145-  158 (26.14/20.34)	TYNRLPTCIRERIV
  171-  184 (23.62/17.49)	TLLRLNQVIQHRLV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      35.37|      10|      26|    1422|    1431|      12
---------------------------------------------------------------------------
 1422- 1431 (18.52/11.12)	KEWKDSPSLV
 1449- 1458 (16.84/ 9.37)	REQVQSPAAV
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04553 with Med14 domain of Kingdom Metazoa
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