<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04541

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMSFHLSTKERLLLLIDDMELISKELIDNLSPKHQKISTAEHTALVDLLVAKDEEFRKMLELADEQAKLEQKMDDMKAKVDVQDREIQKLQKSLKEAEHILSTAIFQARRKLASINQANKRPVSSEELIKYAHRISSANAVSAPLTWCIGDVRRPYPTDIEMRNGFLGKSDLNINGGGVAHQSNMAENQRNMNEVPNAFQNQFNWTNMGELHMSMGSSGNSVALETRSHKEASQDDVEVMSTDSSSSSSSDSQ
Length252
PositionMiddle
OrganismLucilia cuprina (Green bottle fly) (Australian sheep blowfly)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Endopterygota> Diptera> Brachycera> Muscomorpha> Oestroidea> Calliphoridae> Luciliinae> Lucilia.
Aromaticity0.04
Grand average of hydropathy-0.625
Instability index51.20
Isoelectric point5.53
Molecular weight28259.46
Publications
PubMed=26108605

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP04541
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      84.59|      28|      33|      17|      46|       1
---------------------------------------------------------------------------
   17-   46 (41.98/31.32)	DMELISK.ELIDNLSPKHQKIStaEHTALVD
   52-   80 (42.61/25.82)	DEEFRKMlELADEQAKLEQKMD..DMKAKVD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      89.85|      29|      42|      81|     109|       3
---------------------------------------------------------------------------
   81-  109 (46.11/30.32)	VQDREIQKLQKSLKEAEHI...LSTAIFQARR
  122-  153 (43.74/28.46)	VSSEELIKYAHRISSANAVsapLTWCIGDVRR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      39.88|      12|      14|     223|     234|       4
---------------------------------------------------------------------------
  223-  234 (20.12/10.74)	LETRSHKEASQD
  239-  250 (19.75/10.43)	MSTDSSSSSSSD
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04541 with Med4 domain of Kingdom Metazoa
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