<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04176

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMQAEQKQPVDHSGVPVEALEALRLRLTQLTHSLNKLQAEFKNPQLTHYVSLQAQLSIILTQLGSLSSTLSSYSDVLERTVAYPLPTFPTTEQEGLLTTLLRKKATPEVVEWIEESKSKNTDFLLKDDEDITKWAARCTVDTKEKYNFVGFKTKQEEREANGDDEKDVDIADEPAASSKYSVDQILKFMYQGDITK
Length195
PositionHead
OrganismCyberlindnera jadinii (strain ATCC 18201 / CBS 1600 / CCRC 20928 / JCM 3617 / NBRC 0987 / NRRL Y-1542) (Torula yeast) (Candida utilis)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Phaffomycetaceae> Cyberlindnera.
Aromaticity0.07
Grand average of hydropathy-0.572
Instability index40.74
Isoelectric point4.92
Molecular weight22070.55
Publications
PubMed=26150016
PubMed=27535936

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364144
GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
protein-macromolecule adaptor activity	GO:0030674	IEA:EnsemblFungi
RNA polymerase II cis-regulatory region sequence-specific DNA binding	GO:0000978	IEA:EnsemblFungi
TBP-class protein binding	GO:0017025	IEA:EnsemblFungi
transcription corepressor activity	GO:0003714	IEA:EnsemblFungi
GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP04176
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      81.03|      28|      32|       2|      33|       1
---------------------------------------------------------------------------
    2-   33 (35.67/35.72)	QAEQKQP.VDHSgVPVEAleALRLRLTQLThSL
   37-   65 (45.36/27.23)	QAEFKNPqLTHY.VSLQA..QLSIILTQLG.SL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04176 with Med8 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




NANANA









Molecular Recognition Features






MoRF SequenceStartStop




1) KDVDIADEPA
2) KYSVDQILKFMYQGDIT
165
178
174
194