<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP04129

Description Mediator of RNA polymerase II transcription subunit 16
SequenceMPLMLDNPMETTTHDGDQVMNHGAISAGDIPMNGPMQVDGLDVDDLFGDGVNLTLPNVRGPSRQLAQRVDEMRTRGCAQGIAWSKSGTIASISPDGRSVQVRYLRADPKTGGWGLSEPTTVDAITASPSTPIVHLAWAATISNELAVIDAAGRVAILAFSVSLNRPFLTRKWDADHVDDLHAIAGCFWLPLASQHKNVRAPSVFGRRLCASTLTISQYHVFHGPAVKDATTYRYESSFIHAFGPWHPNPAKSALLCVTTSGMLRLFWSQSNNKLEETTLELDSFSSSDDLITHASLHSDKSKPFRETLPVYACSQILDHMYVALATASRQLRLVRVSISWGPPQPDNKLPPSGQPLNPTMTAKHLAISSWMPNGQSGNPIDASMTQISHIELLPSQLDNTGQNWVPMNVLVVRSYLPTPNSPFQEMQSVIDRWEVGHEPSPALHPAFEQMGSRRNSNAGGNDKDPNRLKKLDPVFINKVIVGMQTIHFGKAVCIAFSDGSVEYRDRFTMQETYNELSLDRVMTLNQVGFTFAEGSPCLQAAFSPTNCSVAQIQDDGQVKWNGLHYPLGDIGNSNQDAQYAAVIAALTVAASAATFYQVNCDDILAAAKPYADKKCKSIIINHLGFKGEFNPRSSSGKFAFLSLNIRNVVILITIASNTPTNMREKLNPLDEHEVIDALCGCAQWSVDLLAYLADSLFALLDDPKFTELLQPRNYSEISNYLRSRSDPSLQLLLCSATRGFLSAVCRRLTHLDQISHRAIEFYDRRAAMQNATDPSGQSKVATIALNKSYQRMQHIISNSHIKVQDFEHLLTTLTSDIRSTYQTAFAGLAAQAQQKNESNGTANTQQGQKQIDAQIRGAQIISELNLLLAAQPPPPFLTVLKKLFSQDLRAFRAQTDPSKLFFADLGLLEVDDAPRVLGARRAAARYVDVFRRVEMTNKAGPKEETPEEQLQQRGGRGGPLQWRRCVRCAAVMEDVFGSRPGFTFVLTQQRKCSCALVRAIAPDGQEHTPERREALAALKGEGEXDAPSPSPDAEDSTMYMNGGHDFAESSPSADENNASDDADFDMDDDLPNVQSDGAVDALSSSNDSQRPSKRKAGVEVQEDLYIKANPELYGLRRSTRPREQRKIVDSDDSESDVPVNRRNVKRRRVETSQRSSKVNTPIRQASADDSDSDNYGGARAKTLQKKARRQREAQPSSLLAEKRWSSRRAAQTVQQGGYEESDFEDEEDEAAQDAQYYAPEADNSPYIDKVLRHRLKDGLELSFESTRQDFEYYIKWQGKSHLHDTWETAQTLRDVRGYRKLENYFRVVVDHELYIRFGFDIPPETKEQFFLDRERVEEALEDYTKVDRVVAVRDGDEETEYLVKWKGCYYDECTWEVASAISTDFQDKIDQFLDRSSRQWVSDRTETNPDTRTRMTKLEAQPDYIKGGELRTFQLRGLNFLCLNWTRANNVILADEMGLGKTVQSVSFMSWLRNDREQEGPFLIVAPLSVIPAWGDTFDNWSPDMNYVVYLGNEASRQTIRENELMINGNSKKPKFNALITSYEMILHDWSFLQTIKWQALLVDEAHRLKNKESQLYAKLVSFGVPCKILITGTPIQNNLAELSALMDFLNPGKVIIDEELETLTGADTQEKLQDLHTSIAPYILRRTKETVESDLPPKTEKIIRVELSDVQLEYYKNILTRNYAALSDATGQKNSLLNIMMELKKVSNHPYMFGGAEDRVLAGSTRREDQVKGLIASSGKMMLLDQLLTKLKKDGHRVLIFSQMVKMLDILGDYLALRGYKFQRLDGTIAAGPRRMAINHFNAEGSDDFCFLLSTRAGGLGINLMTADTVVIFDSDWNPQADLQAMGRAHRIGQKKPVSIYRLVSKETVEEEVLERARNKLLLEYLTIQAGVTDEGKAFRDEFNKKGLKMDGPSSADDIQWILKMRSQKMFEQTGNQERLEQLDIDSILENAEVTKTKVDDKMNLSSGGIDWDNFMQYTDVKVDDLALDWDQIIPAEELENIKAEEEKRRHEAYLAKVAAESAPRRAAVKNRHNENDRADRVAKKRQREEQQRQEAEEQRALLSDPRRPLNEKETRNLLKAFYRYGAMEDRGDEIVHEARLTERDREFLSSIIDDFTKVCGQALDDNYSKLEEDERKLGKSLTKKDKKAVLIDFGEVRKMNAETAIERPKQLRLLRQVLRKNNDDKSFRLPDASKAAHYSCEWGAREDGMLLVGIDRYGFGAWTQIRDDAGLDLQDKFFLEEHRVEKKEERNKASEKSINSPGAVHLVRRSEYLLSVLQSKHSTDAATLKAVENHHRNNKKHLNGGRRSELGSAAASPAPHLSKKNSSHRDRERERDHHRSRSNVDDRGTPRPESKRKHSGHDEGRIPKHRRTDDSRRSKGHADDRKSPRPEKRRHREEDGDRKHRRPDESRHKDDRRRSHDNSQAHDEDRERRNQALARLADLRLMGDSREEREKDPDAMLWYLLKPVRENFEIILGTTKDKVKSSKERARIFGSELVVIGSFLDREFVRESDKPLKNRFWEFLATLWPVDSTSNPVTADRLSFLYRTIRDREQSDAKKKAQNSNGTAATS
Length2573
PositionTail
OrganismVerticillium longisporum
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Hypocreomycetidae> Glomerellales> Plectosphaerellaceae> Verticillium.
Aromaticity0.07
Grand average of hydropathy-0.667
Instability index44.88
Isoelectric point6.33
Molecular weight290920.35
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364149

GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
ATP binding	GO:0005524	IEA:UniProtKB-KW
DNA binding	GO:0003677	IEA:UniProtKB-KW
hydrolase activity	GO:0016787	IEA:UniProtKB-KW
nucleosome-dependent ATPase activity	GO:0070615	IEA:InterPro

GO - Biological Process








Interaction





Binary Interactions











Repeat regions





Repeats

>MDP04129
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     147.77|      33|      35|    2341|    2375|       1
---------------------------------------------------------------------------
 2341- 2375 (60.30/39.60)	RSRSNVDDRGTPRPESKRKHSGHDEGRipKHRRTD
 2379- 2410 (61.03/33.77)	RSKGHADDRKSPRPE.KRRHREEDGDR..KHRRPD
 2412- 2432 (26.44/ 9.29)	.SRHK.DDR...R.RSHDNSQAHDEDR........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.81|      21|     134|      57|      85|       4
---------------------------------------------------------------------------
   57-   85 (29.97/37.35)	NVRGPSrQLAQRVdemrtrgCAQGIAWSK
  197-  217 (37.84/22.08)	NVRAPS.VFGRRL.......CASTLTISQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     113.05|      32|      34|    1103|    1136|       6
---------------------------------------------------------------------------
 1105- 1127 (30.66/17.60)	..........YIKANPELYGLRRSTRPREQRKI
 1128- 1158 (47.65/27.76)	VDSDDSESD..VPVNRRNVKRRRVETSQRSSKV
 1165- 1192 (34.74/18.01)	ASADDSDSDnYGGARAKT..LQK..KARRQRE.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      88.73|      29|      40|    2004|    2042|       7
---------------------------------------------------------------------------
 2004- 2038 (38.36/43.00)	KAEEEKRRHEAYLAKvAAESAPRRAAvknrhNEND
 2047- 2075 (50.37/25.99)	RQREEQQRQEAEEQR.ALLSDPRRPL.....NEKE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     107.97|      32|     312|     453|     486|       8
---------------------------------------------------------------------------
  453-  486 (51.85/43.17)	RRnsNAGGNDKDPNRLKKLDPVFINKVIVGMQTI
  764-  795 (56.12/39.61)	RR..AAMQNATDPSGQSKVATIALNKSYQRMQHI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     367.76|     118|     312|     288|     422|       9
---------------------------------------------------------------------------
  288-  422 (183.42/142.97)	DDLITHASLHSDKS.KP.......FRETLPVYACSQildhmyvALATASRQLRLVRVSISWGPPQPDNklppSGQPLNPtmTAKHLAISSWMPNGQSGNPIDASMTQiSHIELLPS.QLDNTGQnwvPMNVLVVRSYLPTPNSP
  601-  727 (184.33/105.37)	DDILAAAKPYADKKcKSiiinhlgFKGEFNPRSSSG.......KFAFLSLNIRNVVILITIASNTPTN....MREKLNP..LDEHEVIDALCGCAQWSVDLLAYLAD.SLFALLDDpKFTELLQ...PRNYSEISNYLRSRSDP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     191.45|      40|      40|    1271|    1310|      10
---------------------------------------------------------------------------
 1235- 1264 (38.60/20.37)	................QYYAPEADNSPYID.....KVLR.H.RLKDGLELSFE
 1265- 1306 (66.05/40.52)	STRQ..........dfEYYIKWQGKSHLHDTWETAQTLR.DVRGYRKLENYFR
 1307- 1345 (52.52/30.59)	VVVD...........hELYIRF.GFDIPPETKE.QFFLD.RERVEEALEDYTK
 1346- 1393 (34.28/17.20)	..VDrvvavrdgdeetEYLVKWKGCYYDECTWEVASAIStDFQD..KIDQFL.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.50|      20|     305|    2123|    2148|      11
---------------------------------------------------------------------------
 2088- 2107 (33.91/13.17)	AMEDRGDEIVHEAR.....LTERDR
 2124- 2148 (26.59/22.43)	ALDDNYSKLEEDERklgksLTKKDK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      44.76|      14|      21|    1956|    1975|      12
---------------------------------------------------------------------------
 1956- 1975 (17.31/24.42)	TKTKVDDkmnLSsggIDWDN
 1980- 1993 (27.45/14.52)	TDVKVDD...LA...LDWDQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     114.41|      26|      32|    2277|    2302|      16
---------------------------------------------------------------------------
 1074- 1094 (29.87/16.61)	...QSDGAVDA..LSSSNDSQRPSKR
 2277- 2302 (41.39/26.46)	SVLQSKHSTDAATLKAVENHHRNNKK
 2310- 2335 (43.15/27.97)	SELGSAAASPAPHLSKKNSSHRDRER
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      90.73|      32|      33|    1644|    1676|      17
---------------------------------------------------------------------------
 1644- 1663 (15.93/11.55)	...............ILRRTKETVeSDLPPKTEKI
 1664- 1697 (45.54/34.56)	IRVELSDVQLEYYknILTRNYAAL.SDATGQKNSL
 1700- 1721 (29.25/18.29)	IMMELKKVSNHPY..MFGGAEDRV...........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      70.43|      18|      35|    1518|    1535|      18
---------------------------------------------------------------------------
 1518- 1535 (29.72/16.93)	QTIRENELMINGNSK.KPK
 1538- 1551 (16.39/ 6.01)	ALITSYEMILHDWS.....
 1554- 1572 (24.32/12.50)	QTIKWQALLVDEAHRlKNK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.17|      16|      65|     940|     957|      20
---------------------------------------------------------------------------
  940-  956 (25.44/21.43)	GpKEETPE..EQLQQRGGR
 1004- 1021 (23.73/ 8.47)	G.QEHTPErrEALAALKGE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.08|      12|      20|    2159|    2170|      23
---------------------------------------------------------------------------
 2159- 2170 (20.63/12.65)	RKMNAETAIERP
 2181- 2192 (21.45/13.49)	RKNNDDKSFRLP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP04129 with Med16 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AAQTVQQGGYEESDFEDEEDEAAQDAQYYAP
2) MPLMLDNPMETTTHDGDQVMNHGAISAGDIPMNGPMQVD
3) PPQPDNKLPPSGQPLNPTMTAKHLAISSWMPNGQSGNPIDASMT
4) PRRAAVKNRHNENDRADRVAKKRQREEQQRQEAEEQRALLSDPRRPL
5) STDAATLKAVENHHRNNKKHLNGGRRSELGSAAASPAPHLSKKNSSHRDRERERDHHRSRSNVDDRGTPRPESKRKHSGHDEGRIPKHRRTDDSRRSKGHADDRKSPRPEKRRHREEDGDRKHRRPDESRHKDDRRRSHDNSQAHDEDRERRNQALARLADLRLMGDSRE
6) VRAIAPDGQEHTPERREALAALKGEGEXDAPSPSPDAEDSTMYMNGGHDFAESSPSADENNASDDADFDMDDDLPNVQSDGAVDALSSSNDSQRPSKRKAGVEVQEDLYIKANPELYGLRRSTRPREQRKIVDSDDSESDVPVNRRNVKRRRVETSQRSSKVNTPIRQASADDSDSDNYGGARAKTLQKKARRQREAQPSSLLAEKRWSS
1209
1
342
2025
2284
997
1239
39
385
2071
2453
1206










Molecular Recognition Features






MoRF SequenceStartStop




1) EVWEAADELFI
242
252