<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP03932

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMQDDARPRTPSVNPGKQRRKYFTTKPYTLEVTNEPASAYRTHPFQSFVCPPSGSTHNTRPVKHDITLVPLLQKNASPRLMDAKKAALNGEHMVDDAPTIAQPQPPPPLPPNPAEQPVSLRVLVQRLARSGFAELQEVVERLSQAPAYSHRPTLLPHLLTIRQHLLQLLILTQYATKSSEHASLLRSIDAYLEGHNLSFHHLVDFELGPLVKEAAALRTPPPDLEVALRVFKGLPTTNLGRETEGLVGPPPLTPADVNEALEDLNAQIRWRLAMDDTLPMAWWTTQDQWIIHDGRVRFVSEETEGACGWWLELSLFQSHWYLANFGWTDLLEGNGDESDGKMGLPAPQFLEVEASMTRSGVFHSPQPLVSIYTFLSYLAAARRHDRLGRALGNLRWGGTPLWIQLTGPKRQDMRIEYWAGDKAAVEISMKEVEPSAEDRMVCDAEDKVVASSRIEVRRVWKREVGGEEVPLRCGGEAVDVIKAAVGLHVRYVVESIAEALGVDGGFTFAMREGKGGTGIGPVVDVTVCEGVSVTISIDPFTGKKVLSGTIRPNFLRAAESGMNEPAKPVPSYNQAQTGPWANVVEALKKLRFEVVKDGLETRARFLGWEGHDIRALGLTGDDSLAVGRTVHPTCRAKWASWFSVDADAAAAKGWFIVAVMDRVEGGGAGVGGGVGVRWVLARCAGVEGAWRVKSTELIPEVFAPPVVGDENRKRKRDEEPARPSSGSHARGVPGLEFMAKVKRYAKLRKALLQAEDGLIAKGVAYDVLDNGDSLREVKVQRVTMKAASVEGLSMDESPSFENSFVDVLEDGDGVKLVLQGRLKERVVVPDLAPAERAEEDVKVVYDVDSGRVTICVADGEDAGAMLCRKWAAIAKSVNLVKQTLAKPSEYFKLIGFQLGWVRLGYQQGLWVDIGDEIQFGRDGTEGAANPHGLIKRFAGMFSGDLEKLTMLLQLTYPLLTALESIRSTAETDSTISVALAPSLFRIMYTEGPHSLEICFAPRSEADRTTILLLRDAAMKTRIEKAKGERDFKEMVWLHDLWQQGIGANDGIKPLGRALQCRPEKGGSVLRLIHEKIIAVLRGGTVKQEPLLSRAYSEPNAWADDAGLDRPQLLFMGREIRLEKERSKRKNSKTIYDQNVQSINHKKPSNIDIEASTSQETSI
Length1161
PositionTail
OrganismSaitoella complicata (strain BCRC 22490 / CBS 7301 / JCM 7358 / NBRC 10748 / NRRL Y-17804)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Taphrinomycotina> Taphrinomycotina incertae sedis> Saitoella.
Aromaticity0.07
Grand average of hydropathy-0.286
Instability index41.46
Isoelectric point6.37
Molecular weight127943.60
Publications
PubMed=21914972
PubMed=24646756

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP03932
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No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     214.29|      74|     142|     440|     580|       1
---------------------------------------------------------------------------
  440-  524 (111.28/87.68)	VCDAEDKV...VASSRIEVRrvwKREVGGEEVPLRC....GGEAVdvikaAVGLHVR..........YVVEsiAEALGVDGGFTFAM..R.EGkGGTGIGPVVDV
  582-  675 (103.01/114.06)	VVEALKKLrfeVVKDGLETR...ARFLGWEGHDIRAlgltGDDSL.....AVGRTVHptcrakwaswFSVD..ADAAAAKGWFIVAVmdRvEG.GGAGVGGGVGV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     226.36|      70|     142|     841|     920|       2
---------------------------------------------------------------------------
  841-  920 (105.78/85.26)	KVVYDVDSGRVTICVADGEDAG..AMLCRKWAAIAKSVNlvKqtlAKPSEYFKligfQLGWVRLGYQQGLWVDIGDEiQFGR
  984- 1055 (120.58/70.65)	RIMYTEGPHSLEICFAPRSEADrtTILLLRDAAMKTRIE..K...AKGERDFK....EMVWLHDLWQQGIGANDGIK.PLGR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.05|      12|      37|     765|     776|       4
---------------------------------------------------------------------------
  765-  776 (21.14/11.64)	DVLDNGDSLREV
  805-  816 (20.91/11.44)	DVLEDGDGVKLV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.53|      25|      26|     212|     237|       5
---------------------------------------------------------------------------
  181-  205 (39.61/19.34)	ASLLRSIDAYLEGHNLSFHHLVDFE
  213-  237 (42.92/26.69)	AAALRTPPPDLEVALRVFKGLPTTN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      84.24|      27|     522|       9|      36|       7
---------------------------------------------------------------------------
    9-   36 (46.20/40.84)	TPSVNP..GKQRRKYfTTKPYTLEVT....NEPA
  533-  565 (38.04/26.88)	TISIDPftGKKVLSG.TIRPNFLRAAesgmNEPA
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Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP03932 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MQDDARPRTPSVNPGKQRRKYFTTKPYTLEVTNEPA
2) RLMDAKKAALNGEHMVDDAPTIAQPQPPPPLPPNPAEQPV
3) RTHPFQSFVCPPSGSTHNTRPVKHDITLVPL
1
78
40
36
117
70










Molecular Recognition Features






MoRF SequenceStartStop




1) PGKQRRKYFTTKPYTLEVT
14
32