<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP03274

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCLELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQSCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEGESHNKIQIFFLPIVLHRYGVSLCHPGHPSLLLDSKNGVFPCCSLYISVCQTMGCTSNSCKYLIERCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKANNQADTVDFSIISVAGKALGPTDLMEHHSGSQGPLMTTGDLGKEKTQKRVKEGNGTSNSTLAGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKIPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
Length1580
PositionMiddle
OrganismChlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Cercopithecidae> Cercopithecinae> Chlorocebus.
Aromaticity0.04
Grand average of hydropathy-0.674
Instability index53.77
Isoelectric point9.01
Molecular weight168482.46
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


GO - Cellular Component
mediator complex	GO:0016592	IEA:Ensembl
nucleolus	GO:0005730	IEA:Ensembl
nucleoplasm	GO:0005654	IEA:Ensembl

GO - Biological Function
DNA binding	GO:0003677	IEA:Ensembl
estrogen receptor binding	GO:0030331	IEA:Ensembl
LBD domain binding	GO:0050693	IEA:Ensembl
nuclear receptor coactivator activity	GO:0030374	IEA:Ensembl
peroxisome proliferator activated receptor binding	GO:0042975	IEA:Ensembl
promoter-specific chromatin binding	GO:1990841	IEA:Ensembl
protein-containing complex binding	GO:0044877	IEA:Ensembl
retinoic acid receptor binding	GO:0042974	IEA:Ensembl
thyroid hormone receptor binding	GO:0046966	IEA:Ensembl
transcription corepressor activity	GO:0003714	IEA:Ensembl
vitamin D receptor binding	GO:0042809	IEA:Ensembl

GO - Biological Process
androgen biosynthetic process	GO:0006702	IEA:Ensembl
angiogenesis	GO:0001525	IEA:Ensembl
animal organ regeneration	GO:0031100	IEA:Ensembl
brain development	GO:0007420	IEA:Ensembl
cell morphogenesis	GO:0000902	IEA:Ensembl
cellular response to epidermal growth factor stimulus	GO:0071364	IEA:Ensembl
cellular response to hepatocyte growth factor stimulus	GO:0035729	IEA:Ensembl
cellular response to thyroid hormone stimulus	GO:0097067	IEA:Ensembl
embryonic heart tube development	GO:0035050	IEA:Ensembl
embryonic hemopoiesis	GO:0035162	IEA:Ensembl
embryonic hindlimb morphogenesis	GO:0035116	IEA:Ensembl
embryonic placenta development	GO:0001892	IEA:Ensembl
enucleate erythrocyte development	GO:0048822	IEA:Ensembl
epithelial cell proliferation involved in mammary gland duct elongation	GO:0060750	IEA:Ensembl
fat cell differentiation	GO:0045444	IEA:Ensembl
intracellular steroid hormone receptor signaling pathway	GO:0030518	IEA:Ensembl
keratinocyte differentiation	GO:0030216	IEA:Ensembl
lactation	GO:0007595	IEA:Ensembl
lens development in camera-type eye	GO:0002088	IEA:Ensembl
liver development	GO:0001889	IEA:Ensembl
mammary gland branching involved in pregnancy	GO:0060745	IEA:Ensembl
mammary gland branching involved in thelarche	GO:0060744	IEA:Ensembl
megakaryocyte development	GO:0035855	IEA:Ensembl
monocyte differentiation	GO:0030224	IEA:Ensembl
mRNA transcription by RNA polymerase II	GO:0042789	IEA:Ensembl
negative regulation of apoptotic process	GO:0043066	IEA:Ensembl
negative regulation of keratinocyte proliferation	GO:0010839	IEA:Ensembl
negative regulation of neuron differentiation	GO:0045665	IEA:Ensembl
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:Ensembl
peroxisome proliferator activated receptor signaling pathway	GO:0035357	IEA:Ensembl
positive regulation of erythrocyte differentiation	GO:0045648	IEA:Ensembl
positive regulation of G0 to G1 transition	GO:0070318	IEA:Ensembl
positive regulation of gene expression	GO:0010628	IEA:Ensembl
positive regulation of hepatocyte proliferation	GO:2000347	IEA:Ensembl
positive regulation of interferon-gamma-mediated signaling pathway	GO:0060335	IEA:Ensembl
positive regulation of intracellular estrogen receptor signaling pathway	GO:0033148	IEA:Ensembl
positive regulation of keratinocyte differentiation	GO:0045618	IEA:Ensembl
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IEA:Ensembl
protein import into nucleus	GO:0006606	IEA:Ensembl
regulation of vitamin D receptor signaling pathway	GO:0070562	IEA:Ensembl
retinal pigment epithelium development	GO:0003406	IEA:Ensembl
thyroid hormone generation	GO:0006590	IEA:Ensembl
thyroid hormone mediated signaling pathway	GO:0002154	IEA:Ensembl
ventricular trabecula myocardium morphogenesis	GO:0003222	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP03274
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
            10|     566.60|      65|      65|    1149|    1213|       2
---------------------------------------------------------------------------
  613-  657 (45.81/ 8.54)	GSTIG..SSPTPPHHTPPPVS.....S......MAG.NT.................................KNH.P.MLMN..LL......K.DNP...AQDFS...T..........................................................
  660-  717 (54.66/11.73)	....G..SSPLERQNSSSGSPrmeicS......GSN.KT.................................KKK.KSSRLPPE................KPKHQ...T.........................E......D....D...FQREL...FS.M.DVDS
  728-  793 (57.89/12.89)	ADTLD..TPHITP.APSQCST.....P......PTT.YP.................................QPV.PHP..QPSIQ......RmVRL...SSSDS...I.........................G......P....D...VTDILsdiAEEASKLPS
  802-  877 (50.85/10.35)	GTPLR..DSSSSGHSQSTLFD.....SdvfqtnNNE.NP.................................YTD.PADLIADAAG..............SPSSD...S.........................PtnhffhD....GvdfNPDLL...NSQSQSGFG
  982- 1047 (50.62/10.27)	AG.PGldSKP....GKRSRTP.....S......NDG.KS.................................KDK.P.....PKRK......K.ADTegkSPSHSssnR.........................P....ftPptstG...GSK......SPGSSGRS
 1048- 1122 (40.77/ 6.73)	QTPPGvaTPPIPKITIQI....................P.................................KG....TVM...VG......K.PS......SHS...QytssgsvsssgskshhshsssssssS......A....S...TSGKM...KSSKSEGSS
 1149- 1212 (114.42/33.21)	GGKPG..SSPITKHGLSSGSS.....S......TKM.KP.................................QGK.PSSLMNPSLS......K.PNI...SPSHS...R.........................P......P....G...GSDKL...ASPMKPVP.
 1267- 1329 (52.16/10.82)	...AS..SSSFSSSGSSMSSS.....Q......NQHgSS.................................KGKsPSRNKKPSLTavidklK.HGV...VTS...............................G......P....G...GEDPL...DGQM....G
 1335- 1384 (56.55/12.40)	SSHPM..SS...KHNMSGGEF.....Q......GKR.EK.................................SDK.DKSKVSTSGS......S.VD.......................................................SSKK...TSESKNV.G
 1463- 1556 (42.86/ 7.48)	ESESG..SSIAEKSYQNSPSS.....D......DGI.RPlpeystekhkkhkkekkkvkdkdrdrdrdkdrdKKK.SHSIKPESWS......K.SPI...SSDQS...L................................S....M...TSNTI...LSADRP...
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      90.50|      25|      30|     327|     356|       3
---------------------------------------------------------------------------
  331-  356 (41.49/39.07)	ETQPTYAPLYELITQFELSKDPdPIP
  359-  383 (49.01/28.34)	HNMRFYAALPGQQHCYFLNKDA.PLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      94.94|      19|      19|    1213|    1231|       4
---------------------------------------------------------------------------
 1213- 1231 (39.56/18.34)	GTPPSSKA.KSPISSGSGGS
 1235- 1251 (30.60/11.96)	GTSSSSGM.KS..SSGLGSS
 1422- 1441 (24.78/ 7.81)	PQMASSKNyGSPLISGSTPK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     183.06|      59|      70|     154|     213|       7
---------------------------------------------------------------------------
  154-  213 (98.16/71.30)	LKGLVNLYNLPgDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAG...PLDKILHGS..V.GYLTP
  222-  286 (84.91/56.18)	LKYYVSPSDLL.DDKTASPIILHENNVSRSLGMNASVTIEGTSAMyklPIAPLIMGShpVdNKWTP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      65.03|      17|      21|     539|     556|       8
---------------------------------------------------------------------------
  539-  556 (30.58/17.18)	PPASSPGYGMTTGNNpMS
  561-  577 (34.45/15.14)	PTNTFPGGPITTLFN.MS
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP03274 with Med1 domain of Kingdom Metazoa
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