<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP03270

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMAASSSGEKEKERLGGGLGVASGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDSDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSSDMSMNMLPPNHSSDFLLEPPGHNKENEDDVEIMSTDSSSSSSESD
Length270
PositionMiddle
OrganismChlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Mammalia> Eutheria> Euarchontoglires> Primates> Haplorrhini> Catarrhini> Cercopithecidae> Cercopithecinae> Chlorocebus.
Aromaticity0.03
Grand average of hydropathy-0.612
Instability index50.73
Isoelectric point5.02
Molecular weight29748.10
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364141

GO - Cellular Component
mediator complex	GO:0016592	IEA:Ensembl
nucleoplasm	GO:0005654	IEA:Ensembl

GO - Biological Function
thyroid hormone receptor binding	GO:0046966	IEA:Ensembl
transcription coactivator activity	GO:0003713	IEA:Ensembl

GO - Biological Process
positive regulation of transcription initiation from RNA polymerase II promoter	GO:0060261	IEA:Ensembl
transcription by RNA polymerase II	GO:0006366	IEA:Ensembl









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP03270
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      38.34|      12|      24|      25|      36|       1
---------------------------------------------------------------------------
   25-   36 (19.54/10.37)	STRERLLSALED
   51-   62 (18.80/ 9.75)	SRNQKLLQAGEE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     116.27|      28|      28|      94|     121|       2
---------------------------------------------------------------------------
   64-   91 (37.82/22.57)	QVLELLI.HRDGEFQELMKlALNQGKIHH
   94-  121 (43.48/26.88)	QVLEKEVEKRDSDIQQLQK.QLKEAEQIL
  124-  149 (34.98/20.41)	AVYQAK.EKLKS.IEKARK.GAISSEEII
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.54|      23|      23|     216|     238|       3
---------------------------------------------------------------------------
  216-  238 (43.71/24.22)	LAPQYPWQSSDMSMNMLPPNHSS
  242-  264 (38.82/20.85)	LEPPGHNKENEDDVEIMSTDSSS
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP03270 with Med4 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) TWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAG
2) WQSSDMSMNMLPPNHSSDFLLEPPGHNKENEDDVEIMSTDSSSSSSESD
166
222
210
270










Molecular Recognition Features






MoRF SequenceStartStop




1) EPPGHNKENEDDVEIM
243
258