<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP03242

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMESSGHNGTRTDHDRDSSMNGVNGANGTPQDSGFPKEKASAATATDGSAKAINGNDESRSQESNQNGEAERVISRMNDLPDEIVHITQGFVPLSLLLTRLAQTSHNALQDKVAEMAKMPLPAAAANGSSSYSSSGPDDSSADNLRKKGALTNFAQEWHGKWLKALVIAEWSRKAHLVSKLIDLKFHIDQQRILYDTALDEMVNVKRDLTFARMPSPDLKTALQVLSTGNAPWMPDLGYIEPPTLTPEVQLKWINELNTLLSLRLNLDDFDKIPYHFRNYEISSGRVTFKVEGEFEVDLTIADEDFEKQFWFIDFRYAFTPAASSISESLRNYLENCVNDVLGKEGLLGCYQFLHELVLTTKIHELKRQAMQLSRTSWTGTLNVEPLNRALSIQYWTGRSTTMGSKSWIIVAVNSNRSRNGKEAASSTSQLVVNWYRDNKEVKDVEIEFNVNELSAEALLTNVIARHIEHILTSIHDKLLTVARFKNHEASMALRVSRTDPAFSSLTTQVGHSGEASMLLEPMTGVFALKPPSRFTIQPEHQLNMGKTPAEDGLNCLEVLRCAVMEDELHCRATLMGWFVRKPPMAAEELKSAIKVRDWTRVICLQKDGWGSNWFVVVVLSLNGDEWWLLESNPNEASKTLRFKAKLPINNGHPDLSDVFWENLAFFTTGVIAQSVDLRELHRQKIKSRSSNRISLSTSQTVRLPSTDVALSALFPSMVFDQSKPEEDALSNSEGQLSNLELLSLIQQASGAVLVRKKAWADNIVSITFRGIRCLSKAENADDGSKENELICTSEAILKVRKPSKFASLDGLADRDVSYNPKRGEFALRIQRAVGRPILDTLKSRIKAIDRFVNFLEALESANGTITTESVTLRQITFYYSELQTKQSSNDADQVMEDSRKRWRVVLDLSKDDIDIEIEKGNPHLRVLDLMRQLVNSDGGIGVLTAWLPASLPALQAIDKMETEWEPIQAAGQGRIEFSMKTVAWMSVEYTMASDAKTLKNPIRLQVQMQPRRSQAWWHVWRSDAEPKADDRVSTALQPIWNKRGDNWLGLSTGAAGRPQDGVVKMLLAVDEAIRSTITSGPGVTQGNEVVVLD
Length1093
PositionTail
OrganismMetarhizium anisopliae BRIP 53293
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Hypocreomycetidae> Hypocreales> Clavicipitaceae> Metarhizium.
Aromaticity0.07
Grand average of hydropathy-0.362
Instability index39.70
Isoelectric point5.89
Molecular weight122065.84
Publications
PubMed=25102932

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP03242
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     103.24|      24|      29|     993|    1016|       1
---------------------------------------------------------------------------
  961-  984 (23.43/ 9.94)	...ETEWEPI..QAAGQG...RiefsmKTVAW
  993- 1016 (44.59/24.96)	SDAKTLKNPIRLQVQMQP...R.....RSQAW
 1022- 1047 (35.21/18.30)	SDAEP.KADDRVSTALQPiwnK.....RGDNW
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      75.13|      22|      29|      16|      37|       2
---------------------------------------------------------------------------
   16-   37 (42.02/28.98)	DSSMNGVNGAN.GTPQDSGFPKE
   46-   68 (33.11/21.14)	DGSAKAINGNDeSRSQESNQNGE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      83.51|      26|      35|     248|     276|       3
---------------------------------------------------------------------------
  248-  276 (37.59/38.41)	VQLKWINELNtlLSLRLNLDDFDKiPYHF
  286-  311 (45.92/33.24)	VTFKVEGEFE..VDLTIADEDFEK.QFWF
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      89.92|      30|     219|     552|     582|       5
---------------------------------------------------------------------------
  546-  576 (48.36/39.49)	KTPAEDGLNClEVLRCAV.MED..ELHC.RATLMG
  577-  610 (41.57/28.43)	WFVRKPPMAA.EELKSAIkVRDwtRVIClQKDGWG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.79|      16|      27|      77|      92|       7
---------------------------------------------------------------------------
   77-   92 (28.56/18.69)	NDLPDEIVHITQGFVP
  106-  121 (27.23/17.50)	NALQDKVAEMAKMPLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.65|      21|      27|     794|     814|       8
---------------------------------------------------------------------------
  794-  814 (33.49/20.22)	EAILKVRKPSKFASLDGLADR
  824-  844 (34.16/20.76)	EFALRIQRAVGRPILDTLKSR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      34.48|      10|      29|     500|     511|      10
---------------------------------------------------------------------------
  500-  511 (14.08/14.32)	PafSSLTTQVGH
  531-  540 (20.40/12.10)	P..SRFTIQPEH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      34.59|      10|      27|     207|     216|      11
---------------------------------------------------------------------------
  207-  216 (19.30/10.65)	DLTFARMP..SP
  235-  246 (15.30/ 6.98)	DLGYIEPPtlTP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP03242 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MESSGHNGTRTDHDRDSSMNGVNGANGTPQDSGFPKEKASAATATDGSAKAINGNDESRSQESNQNGEAERVISRMND
1
78










Molecular Recognition Features






MoRF SequenceStartStop




1) GFPKEKASAAT
33
43