<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP02688

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMNGDRPTANGERIQNGVQQGASDSSSHVGPLKQSREGQAPSTSTADRSSSYANGVPQPSNGDLGYNAANGTNQAPLEILQLIPRDNYLPMAALISRASQSCWNGLTNLVEQLAAVQVPEQSPDQAKVLPNGLPNNQTKANLDKKERLLKFANDQKADFIKLLVLLQWSRDVEEVGKTISINYWLMKRRQAYWDTIALMAGLKQESAGFQMPNPDLRVAAEVLSRGEVLKFPSLGYLPQKDLTSKQILRLLKSLNRLLSVKLALSDDLPPQLRQFHIHDGRATFIVPDEFELDVSILDENPNSQFRVVDFRYSFSPSPYIPDSLHAEIERYANSNIDRGGLQGCYFFLKELALSSKLAEYHKQVLELARSQWAGNLRVELIRRNLVVQYWSERQASKSWMEVGIASGREKREGSAQVAFPVLEIKWMRQGKKVDSLRLRQNESVLCFEDILRQIIAQHSTQILDGVYDKLVLAPLFAEAELSVEQSLSDEDPEQCSLSIQLSRSSTLQLKVDAVTGLLVISPVSEKSERLQYEVNRIHGVADEIVSKLLNFRCSVVESSVLAGISGTSWEILRAFKFSPAEIKALFGGPVVRMNLFRQSQWGVSYSLAVTHGQDGDHWWLLQQMSTSGPNSQSRFQVLRHQRIEAKEELSSAYFDRLAEYAMGLICLRRNADFLKERKEKFNLPSFPPLEKNYELPPISFDLDMARPTKSLLHGAAAETFPGEAAQNPPSKPACLQKSIQVRFGGVDRTTNRVTAIAHFQNRASAAVLKHLDESTLDSDVTLNVEDRTVTIRVKTSITEAAIPRIVDKATDLEKIVSTVEQIHRLPGLKLRSLSNSAFTIIYHQGLSKELGVSIAFGSGSQTPRLEFFPSEDNPHGLLTAQYSKLLVASRVPFATKVRDFLTSLTLTLPLLSFLYKLQRKHGLEVSRSQTTPSQEPERGLRVHVLVRSATAFAVQYFTPARHVPGDVGPNSQPHLLARLEILPHINASKKPMWLVRAALEEFQSNSRPSYSTPELRTQLRQEIFTRTDGQKWLALDSAAACMADQPEALFQAMHDLLSNWATLAAESKGEKEAKGKAEPKPEATNNLPNGNTKPNVAGKLQAAKLQRPTNPNVSRVVPGKGNPNPPKIEEYIMLD
Length1134
PositionTail
OrganismRhinocladiella mackenziei CBS 650.93
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Chaetothyriomycetidae> Chaetothyriales> Herpotrichiellaceae> Rhinocladiella.
Aromaticity0.07
Grand average of hydropathy-0.366
Instability index47.07
Isoelectric point8.79
Molecular weight126381.30
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP02688
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     285.98|      69|     112|     826|     894|       1
---------------------------------------------------------------------------
  826-  886 (91.35/53.15)	.........GLK.LR....SLSNSAFTIIYHQGLSKELGVSIAFGSGSQ.TPRLEFFP...SEDNPHGLLTAQYSKLLV
  887-  945 (71.47/39.91)	ASRVPFAT....kVRdfltSLTLTLPLLSFLYKLQRKHGLEV...SRSQ.TT.....P...SQEPERGL....RVHVLV
  947- 1003 (60.35/32.50)	.....................SATAFAVQYFTP.ARHVPGDVGPNSQPHlLARLEILPhinASKKPMWLVRAALEEFQS
 1004- 1054 (62.81/34.14)	NSRPSYSTpELR.TQ.....LRQEIFT..RTDG.QKWLALDSAAACMAD.QPEALFQA...MHD...............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     112.34|      32|     600|      48|      89|       4
---------------------------------------------------------------------------
   48-   79 (56.35/26.75)	SSSYANGVPQPSNG..D....LGYNAANGTN.QAPLEIL
  603-  637 (36.08/15.29)	..SYSLAVTHGQDG..DhwwlLQQMSTSGPNsQSRFQVL
  649-  670 (19.90/12.21)	SSAYFDRLAEYAMGliC....LRRNA.............
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     247.72|      70|     203|     147|     240|       5
---------------------------------------------------------------------------
   93-  131 (31.50/37.30)	....................LISRaSQSCWNG...LTNLVEQLAAVQVPeqSPD...QAKVLPNG...............
  166-  240 (109.09/80.07)	QWS..rdVEEVGKTISINYWLMKR.RQAYWDTIALMAGLKQESAGFQMP..NPDLRVAAEVLSRGEvlKFPSLgYLPQKD
  370-  441 (107.12/61.74)	QWAgnlrVELIRRNLVVQYWSERQ.ASKSWMEVGIASGREKREGSAQVA..FPVLEI..KWMRQGK..KVDSL.RLRQNE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     154.60|      49|     395|     674|     728|       6
---------------------------------------------------------------------------
  674-  728 (76.87/65.40)	KERKEKFNlPSfPPLEKNY....ELPPISFDLDMA...RPTksllHGAAAETFPGEAAQNPP
 1070- 1125 (77.73/48.23)	KEAKGKAE.PK.PEATNNLpngnTKPNVAGKLQAAklqRPT....NPNVSRVVPGKGNPNPP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      43.65|      11|      28|     273|     283|       7
---------------------------------------------------------------------------
  273-  283 (22.23/14.60)	QFHIHDGRATF
  303-  313 (21.42/13.81)	QFRVVDFRYSF
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP02688 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ATLAAESKGEKEAKGKAEPKPEATNNLPNGNTKPNVAGKLQAAKLQRPTNPNVSRVVPGKGNPNPPKIEEYIM
2) MNGDRPTANGERIQNGVQQGASDSSSHVGPLKQSREGQAPSTSTADRSSSYANGVPQPSNGDLGYNAANG
1060
1
1132
70










Molecular Recognition Features






MoRF SequenceStartStop




1) PKIEEYIMLD
2) VAGKLQAAKLQRP
1125
1095
1134
1107