<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP02534

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMNGNGERPTVNGDRVLNGLQRTASDTAHSSPSIKNEKSPHPVAAISPGRQQSRPNGDLPRKSENVSVPASEKLSDPLLDAPQDILQLVPRDSYLPVATLINRATQSCWNGLSSLVQQLATIQVPDRTDPDRPLPNGHINNQSDANLQKKERLMRFCYDQRNDFIKLAVLLDWSKNVDHVAKTISINYWLMRQRQAYWDTIAAAAILRQEAAGFQLPNPDLKTAAEILSTGRSAHLPTFGYVPQKPLSNKQILSLLKSLNRKLTVKLALIDNLPPQLKSYHVHDGRVTFTVPDMFELDLSILDDSADSPFRIVDFRLTFSPAPKLSDNLRSEIELCSNSNIDRDGIQGCFLFLHELALSYKLAAYQKQAVELSRTHWAGNLRVEVIRRNLIVQYWTEKPNYKSWIEMGISSGRQPSAMSPVPDLPYLDIKWMRQGKKVHNLALNVTQSTLRFEDILSQVVAQDSTLVIDDIYNSLLLTPLFSNAELCVQQRLSYTDPNECSLTLQLFRSTYLDLRIDPVTGSIVTSPVSEKSERLQFEVNRSQALASDISTKLLNFRASIMESVVLAAVVGTRWEVMRAFKFTQVEIKSLFGGPIVKLDMLRQPQWGPDYFLAITHAHNGDFWRLLRQISHPETHVSTHFSVILSQRIDVNQELTPAYFERLADYTTGFISLRRSADYLAKLTDKYQLPRIPDFGDVFGLPQISFPLDWARPRTATSSSRDASGVSRDRARTAANVINIRFEGVDPSSKEAIFIAKMDTSASTNTLRQLVDPSLDENVLLNPEERSVTIRATSAVSDTAIPTIVTTAIRLETLISTIQQIRHLPGVKLESLSSSGVCVSYYKASDTEELSVRISLGSNGQPPRLEFSPPGDNPHALLASPYSKILAMDDGPTSTKIRDLFTSLMMSYPVVSFLHKLQKRHGAVGETPSTEKDDTLRVHILARTVHQFAVQYFATLPQTAKDIGSDQQPSLIARLEILPHTDRTRKTMWMVRPALEEFQAYARPSFSATALRNKLRQEVLTLTDPPKPWLALDNAAACLTDQPEPLLSAVHDVLSTFVKEANSTPVRPESTSQSSVKQGPGGNASAGGAKAKSQNPANVTTASQGQGRSGGKMQVPGAGPNGVMKPAQQQPLQNQRPNMSQGRPMPNKSTGKANAPNNQDNPITLD
Length1164
PositionTail
OrganismExophiala mesophila (Black yeast)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Chaetothyriomycetidae> Chaetothyriales> Herpotrichiellaceae> Exophiala.
Aromaticity0.07
Grand average of hydropathy-0.361
Instability index46.96
Isoelectric point8.89
Molecular weight129161.16
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP02534
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      62.45|      17|     110|     854|     870|       1
---------------------------------------------------------------------------
  854-  870 (34.88/22.36)	LGSNGQP...PRLEFSPPGD
  961-  980 (27.58/15.95)	IGSDQQPsliARLEILPHTD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     214.88|      59|     110|     531|     593|       2
---------------------------------------------------------------------------
  531-  593 (84.39/61.56)	SERLqfEVNrsQALASDISTKLLNFR.A...SIMESVVLA...AVVGTRWEVMRAFKFTQVEIKSLFGGP
  596-  640 (46.96/23.45)	....................KLDMLRqP...QWGPDYFLAithAHNGDFWRLLRQISHPETHVSTHFS..
  644-  700 (83.53/49.28)	SQRI..DVN..QELTPAYFERLADYT.TgfiSLRRSADYL...AKLTDKYQLPRIPDFGDV.....FGLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     152.36|      48|      51|       4|      52|       3
---------------------------------------------------------------------------
    4-   52 (78.36/50.70)	NGERPTvNGDRVLNGLQRTASDTAHSSPSIKNEKSPH....PVAAISPGRQQS
   55-  106 (73.99/43.56)	NGDLPR.KSENVSVPASEKLSDPLLDAPQDILQLVPRdsylPVATLINRATQS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     470.63|     149|     202|     165|     320|       4
---------------------------------------------------------------------------
  114-  157 (27.98/ 8.47)	.....................................................................................................................LVQQLATIQV.PD.R...TDPDR.PLPNGHINNQSDANLQK..KERLMRFCY
  165-  320 (224.97/162.86)	KLAVLLDWSKNVDH.....VAKTISINYWLmrQRQAY..WdtIAAAAILRQEAAGFQlPNPDLK.TAAEILSTGRSAHLPTFGyVPQKPLSNKQILSLLKSLNRKLTVKlALIDN..LPPQLKSYHV.HDGRVTFTVPDMFELDLSILDDSADSPFRI..VDFRLTFSP
  366-  526 (217.68/134.36)	KQAVELSRTHWAGNlrvevIRRNLIVQYWT..EKPNYksW..IEMGISSGRQPSAMS.PVPDLPyLDIKWMRQGKKVHNLALN.VTQSTLRFEDILSQVVAQDSTLVID.DIYNSllLTPLFSNAELcVQQRLSYTDPNECSLTLQLF.RSTYLDLRIdpVTGSIVTSP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.83|      22|      25|     731|     753|       8
---------------------------------------------------------------------------
  731-  753 (32.28/19.20)	TAANVINIRfEGVDPSSKEAIFI
  758-  779 (34.55/16.29)	TSASTNTLR.QLVDPSLDENVLL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP02534 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FVKEANSTPVRPESTSQSSVKQGPGGNASAGGAKAKSQNPANVTTASQGQGRSGGKMQVPGAGPNGVMKPAQQQPLQNQRPNMSQGRPMPNKSTGKANAPNNQDNPITLD
2) MNGNGERPTVNGDRVLNGLQRTASDTAHSSPSIKNEKSPHPVAAISPGRQQSRPNGDLPRKSENVSVPASEKLSDP
1055
1
1164
76










Molecular Recognition Features






MoRF SequenceStartStop




1) MKPAQQ
1122
1127