<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP02471

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMTTTIGMLQNNGLVNPQVQKVGSVSNGLKAGEMNSKEPGVGKDLKNHNIGNLKQETKSKPVELPHVAVNQEPLALLIRNLTVFSIKELAQFFKTSVHSNQDPSAKKTTFLQLVVFLRNQFLKLYVLVKWCRTLKHNNFNTMIDLLNWFRGSNMTVNNCIWALKASVAGMTNAKLPNPDLITALEVLSLGRPNLPSHNFTLSGEEGAQGGAKKSVVPPKLILKTLRNMNIILSVKISLMEIPDQFRHYTVNDGRLYVVVEDEFEIQLSTTDQASPLFFVDLKLLFNEALPLNKYMLEKVINEVLFKSPKPLFALYKLLHNYVLSVQMYMIHADLLSLESNSKYAGGNLVHHYDSRKTIINIRYWCNSRLKGKCNAIIAVEEASGSIILEWNIEEVANGEIAVPLRYVNVLSNLGNILDEIMFNHSQMIKTELLSTGVLQEDEEVSDALLFNVPSTPASVIPIQIKINTISGVFYVRNPSSLLTFYINQMNKTSTTEEFVKVLNKLKLDKIISILGNMFEKVGWTSNNVVKLDAPVSKDPSWAQKKILFRELFIRLENWPSNWYLILSVISSNTTCMIEKRIGKIAAAKGKWELKYLDSENTSTLKLESMTYQKMIHLKKTSLHKIVDHMIIDSLNGLNMRNRICSRKQLGSIPKYIVPEDQSHHSSVLGIELESFVGGTSALKHILEDTLFLRIDYHISEIKLFGKFRQNCKALGNQYNETLLHFTGEGALSFFMSEPFTDLNNIVQHFNTFKQKLMQIVTLTDVIEELHGFYSENFSIVASKPNEISFKYLKNSKDQYDCRILLHTNDHSVENLDIELSPSNPQHIFQPFIESSKLDYHFVFNYLQFTSGFFGILDSILTTSGGQAKSKNEFTTISLQLHNLSEYELIYYNNQTNTKITLMIELRNVFLNGSKKLQYFVHFAEDEHISTKSPAYPLVHKVRNTVFMVDSTSIIAGDEISSLNSPKRVKPSSLIKLTDGVCCGAAEIASVIGDIHDILKVDCV
Length1002
PositionTail
OrganismLachancea lanzarotensis
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Saccharomycotina> Saccharomycetes> Saccharomycetales> Saccharomycetaceae> Lachancea.
Aromaticity0.09
Grand average of hydropathy-0.098
Instability index38.27
Isoelectric point8.33
Molecular weight113370.65
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


GO - Cellular Component
core mediator complex	GO:0070847	IEA:EnsemblFungi
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
RNA polymerase II repressing transcription factor binding	GO:0001103	IEA:EnsemblFungi
TFIIB-class transcription factor binding	GO:0001093	IEA:EnsemblFungi
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
negative regulation of transcription by RNA polymerase II	GO:0000122	IEA:EnsemblFungi
positive regulation of transcription by RNA polymerase II	GO:0045944	IEA:EnsemblFungi









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP02471
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     232.11|      70|      93|     371|     444|       1
---------------------------------------------------------------------------
  272-  343 (26.73/10.46)	................................ASPLFFVDlkLLFNealplnkymLEKVINEVLFKSPKplfalykllhnyvlsvqmyMI.HADLLS...LESNSKYA
  371-  444 (106.32/80.81)	KCNAI...IAVEEASGsiILEWNIEEVANGeiAVPLRYVN..VLSN.........LGNILDEIMFNHSQ...................MI.KTELLSTGVLQEDEEVS
  464-  535 (99.07/63.91)	KINTIsgvFYVRNPSS..LLTFYINQMNKT..STTEEFVK..VLNK.........LK..LDKIISILGN...................MFeKVGWTSNNVVKLDAPVS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     118.04|      36|      90|     709|     748|       3
---------------------------------------------------------------------------
  709-  741 (53.68/26.41)	...............NCKAL..GN.....QYN.E......TL.LHFTGEGALSFFMSEPFTDL
  742-  779 (29.75/18.51)	NNI.....................vqhfnTFK.QklmqivTL.TDVIEE..LHGFYSENFSIV
  784-  833 (34.61/15.31)	NEIsfkylknskdqyDCRILlhTN.....DHSvE......NLdIELSPSNPQHIF..QPFIES
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     151.14|      48|     228|     647|     698|       4
---------------------------------------------------------------------------
  647-  698 (74.70/46.51)	QLGSIPKY..IVPEDQSHHSSVLGIELES.FVGGTSALKHILEdtlFLRiDYHIS
  878-  928 (76.43/38.00)	QLHNLSEYelIYYNNQTNTKITLMIELRNvFLNGSKKLQYFVH...FAE.DEHIS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      82.62|      25|      26|     105|     130|       6
---------------------------------------------------------------------------
  105-  130 (38.35/27.64)	KKTTFLQLVVFLrNQFLKLYVLVKWC
  134-  158 (44.27/27.59)	KHNNFNTMIDLL.NWFRGSNMTVNNC
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     125.96|      33|      33|      42|      74|       8
---------------------------------------------------------------------------
    8-   38 (27.83/10.86)	..LQNNGLVNPQvQKVGSVSNGLKAGEMNsKEP..
   42-   74 (55.39/27.67)	KDLKNHNIGNLK.QETKSKPVELPHVAVN.QEPLA
   78-  104 (42.75/19.96)	RNLTVFSIKELA.QFFKTS......VHSN.QDPSA
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP02471 with Med14 domain of Kingdom Fungi
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