<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP02284

Description Mediator of RNA polymerase II transcription subunit 4 (Fragment)
SequenceMDKYIDSRFERLEKALSNLIDSVTKYHPSTIQAEELKAADNELSKGLEEVQIHQNNHLRIQKLRQSSAALDTQIRDTLTSLANTHKDIVSTHTTTYPSEPNYPIAYEELLRYARRISKTTLPPAATLEGVAASPETQSPNPESLAQSALTPSARTPSQPQSPAVNGTPQLTAEQPTQPPAANLNTSLPEGMSQYLNPLSGQLFFPWPLEDKIRSGSLASYQVLVEQGIDPKGYDPATEEERRLKEEEERRAKEEQEKAEREAREKQLREERERARAERERQREKEQAEWRRASIVSGQPDGPGLSRAGTALGEKKQFQFTNLDDLDDDDDDDD
Length333
PositionMiddle
OrganismMetarhizium majus (strain ARSEF 297)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Hypocreomycetidae> Hypocreales> Clavicipitaceae> Metarhizium> Metarhizium majus.
Aromaticity0.05
Grand average of hydropathy-1.048
Instability index59.50
Isoelectric point4.93
Molecular weight37500.74
Publications
PubMed=25368161

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP02284
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     122.22|      16|      16|     150|     165|       1
---------------------------------------------------------------------------
   91-  106 (27.99/11.21)	TH..TTTYPSEPNYPIAY
  110-  125 (20.66/ 6.76)	LR..YARRISKTTLPPAA
  133-  147 (22.04/ 7.60)	SP..ETQSPN.PESLAQS
  150-  165 (29.38/12.06)	TP..SARTPSQPQSPAVN
  167-  182 (22.15/ 7.66)	TPqlTAEQPTQP..PAAN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      56.99|      18|      21|     240|     260|       2
---------------------------------------------------------------------------
  240-  257 (30.26/18.19)	ERRLKEEEER.RAKEEQEK
  264-  282 (26.73/ 8.50)	EKQLREERERaRAERERQR
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP02284 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DTQIRDTLTSLANTHKDIVSTHTTTYPSEPNYP
2) LASYQVLVEQGIDPKGYDPATEEERRLKEEEERRAKEEQEKAEREAREKQLREERERARAERERQREKEQAEWRRASIVSGQPDGPGLSRAGTALGEKKQFQFTNLDDLDDDDDDDD
3) LPPAATLEGVAASPETQSPNPESLAQSALTPSARTPSQPQSPAVNGTPQLTAEQPTQPPAANLNTSLPEGMSQYLN
71
217
121
103
333
196










Molecular Recognition Features






MoRF SequenceStartStop




1) KEQAEWRRASIVSGQP
2) PGLSRAGTALGEKKQFQFTNLDDLDDDD
284
302
299
329