<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP02259

Description Mediator of RNA polymerase II transcription subunit 8
SequenceMATLGLDEDELKSVEHIVARLAQLSSSIQSLKMDILKSNPLPHPSSLQASAQILQRNLQSVLESLNDNAELFTRMVIHPSTNYPGRTQENILTQLLRKKLEPDVEELVAEGRETARLATAEGIAALQAIWDELREWTQGRIAAYVREEAGDVYTREEREAGVDNVRTGLRRGLDESDDEDDEAGGGEGEGEDEDEDGDEDGERGAAVVPRGPEPEMLLWFGARGDFELPRNVEFERKVGVRRGLEGINVPPDRMDSAASS
Length260
PositionHead
OrganismMetarhizium album (strain ARSEF 1941)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Hypocreomycetidae> Hypocreales> Clavicipitaceae> Metarhizium.
Aromaticity0.04
Grand average of hydropathy-0.666
Instability index45.34
Isoelectric point4.43
Molecular weight28730.32
Publications
PubMed=25368161

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364144
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP02259
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      49.11|      13|      17|     172|     188|       1
---------------------------------------------------------------------------
  172-  184 (24.33/16.19)	GLDESDDEDDEAG
  192-  204 (24.78/ 6.88)	DEDEDGDEDGERG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      38.16|      13|      17|      26|      39|       2
---------------------------------------------------------------------------
   26-   39 (17.84/18.41)	SSIQSlKMDILKSN
   45-   57 (20.32/14.66)	SSLQA.SAQILQRN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.37|      18|      25|     105|     122|       3
---------------------------------------------------------------------------
  105-  122 (28.40/16.48)	EEL..VAEGRETARLATAEG
  131-  150 (26.97/15.33)	DELreWTQGRIAAYVREEAG
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP02259 with Med8 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) DNVRTGLRRGLDESDDEDDEAGGGEGEGEDEDEDGDEDGERGAAVVPRGPEP
163
214










Molecular Recognition Features






MoRF SequenceStartStop




1) AAVVPRGPEPEMLLWFGARGDFELPRNV
205
232