<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP01863

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMPGLVMDDVSASASRDWGDSYDRHGSNGDSTVMRGAHHVDNLGRSENPQQANGFKGEDGKGQGSSTAMVLKHGQSGVVPDGVEGQWAPPLMHITQGFFPYSQLVNRAVQQCYNDLCDVITELADSTQQQQQQLPSSANGKPDPASIQKRIRILEFAQTKRTEFIKLLVLSQWSRQAVDVSRLIDLQNFIRTRHFAYQTAVQRVADMKRDLVRAQVANPDLQTALEVLTTGRVSNMPEFGYKPPKPLSSRRLLATLQRINRIISTRLVTSDSIPSSLNEYQVHDGRVTFSVHGEFELDLSIAEEDVTSQFYFIDIRFLFTPSSPIPKGRFFNELDSQINGILKTKGLTGCFDFIHNLVLANKINILFKQAMSLSRGQWTGALRVELLHRTLVVHYWPDKSGPKSWLEIGAHSGSHQRQKVSYLALRWIREGKECDFSQIHFDTESLSMESILRSVIAIHSSHILRAVYERLRTQNLFANHRLSINMQMSKTEPGNCRLNVQLTETRYLNASLEPVSGAMCIHTIPSLLCRLDKGSTSDDDFVNRISRLRCIAAMEEIESDAKIFGWESVDHRKFKVDIRRVFPSNILRASFFRNRLWGNSWIIAATTSLSGDDWWILRLKARPSPLPDALGHARVVAGGFSVLSTRVLSGDQLLSPRESRYHFPNLDYSLTGALVMYSNALWLSELGYQNVLPPVEQLQLGSRLEVPCLYLKFDSGKLPKQMRITPLDGTRRKSYVRETLTISYYGIDSRTKNAIVVAHGRFHIPLKKMGLKRLKLDDCILLRRGGSAFAMRFFVTPGQSIMVDLFERIQRLNIVLSLFHTLQQNKITVKSVSLSNLSFVYAPREGFEANIVIRLDGPGHAPDLDPTIIRLQNEPLSRLRLHISFNSLSPHNRIKQSLGMILNRTGPGNGVQCVLQLLKLTLPVLHALDRLVTEPQHNSTLRVQVSIRGAQTYQIHYFGLRFRFLLNASHRRDKIIWALKDISSSEGQRDVEQAVEARLQEKIYCGKGDGWQGLGNGAVAEVYQVGNLLSELDSCFADLTPPAAPPAVPRETAEKKPGKTPKDKGPKTNGTSKVTNRPPQEAPKESDVIMID
Length1091
PositionTail
OrganismTalaromyces marneffei PM1
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Eurotiomycetidae> Eurotiales> Trichocomaceae> Talaromyces> Talaromyces sect. Talaromyces.
Aromaticity0.08
Grand average of hydropathy-0.307
Instability index43.72
Isoelectric point9.45
Molecular weight122671.95
Publications
PubMed=25330172

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	ARBA:ARBA00003669
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP01863
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     287.02|      95|     151|     617|     726|       1
---------------------------------------------------------------------------
  617-  726 (142.46/112.91)	RLKarpspLPDALGHARvvaGGfSVLSTR..VLSGDQLLSPRESRYHFPNLDYSLTGAL...VMYSNALWLSELGYqnVLPPVEQLQ..LGSRLEVPCLYLKFDsgklPKQMRI..TPL
  772-  875 (144.56/81.26)	RLK.....LDDCILLRR...GG.SAFAMRffVTPGQSIMVDLFERIQRLNIVLSLFHTLqqnKITVKSVSLSNLSF..VYAPREGFEanIVIRLDGPGHAPDLD....PTIIRLqnEPL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     160.26|      52|     260|     293|     348|       3
---------------------------------------------------------------------------
  293-  348 (79.54/67.62)	EFELDLSI.AEEDVTSQFYFIDIRFLFtPSSpIPKGRFF.NEL..DSQIngILKTKGLTG
  555-  610 (80.72/52.11)	EIESDAKIfGWESVDHRKFKVDIRRVF.PSN.ILRASFFrNRLwgNSWI..IAATTSLSG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     108.95|      33|     474|     448|     480|       4
---------------------------------------------------------------------------
  448-  480 (55.57/30.93)	ESILRSVIAIHSSHILRAVYERLRTQNLF.ANHR
  937-  970 (53.37/29.47)	NSTLRVQVSIRGAQTYQIHYFGLRFRFLLnASHR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      55.66|      16|     473|     405|     433|       5
---------------------------------------------------------------------------
  387-  402 (30.85/22.66)	HRTLVVHY....WPDKSGPK
  414-  433 (24.81/13.59)	HQRQKVSYlalrWIREGKEC
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      63.85|      20|     187|     162|     181|       6
---------------------------------------------------------------------------
  162-  181 (34.85/24.42)	EFIKLLVLSQWS....RQAVDVSR
  351-  374 (29.00/19.07)	DFIHNLVLANKInilfKQAMSLSR
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP01863 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) ASASRDWGDSYDRHGSNGDSTVMRGAHHVDNLGRSENPQQANGFKGEDGKGQGSSTAMVLKHGQ
2) TPPAAPPAVPRETAEKKPGKTPKDKGPKTNGTSKVTNRPPQEAPKESDVIMID
11
1039
74
1091










Molecular Recognition Features






MoRF SequenceStartStop




1) QEAPKESDVIMID
2) RETAEKKPGKTPKDKGPKTNGTSK
1079
1049
1091
1072