<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP01366

Description Mediator of RNA polymerase II transcription subunit 15 (Fragment)
SequenceDDAMRKAGVAHNKSSKDMESHVFMKAKTREEYLSLVARLIIHFRDIRFWGGSCMAGCILVTIFFVDPMNALQNLTGGPPAGAPGMGMASRAQGAPMSGMSGLGPMGQQMSLPGQQQPPGTSGMAPHGMPGVSTATQQTQLQLQQIAQQQQQQQQQFQQQQVALQQQQFQAQQSAMQQQFQVQQQQAAAAAAAQQQQQQQQQLQAVQQQQQHMLKLQMQQQQNQQQMQQQQQQQQLQRIAQMQQLQAAQAMQAQQQQQQQQQQQQQQIPQQQIQQQPPQQVMQQQMQQMQQQQQQQQQQQQQQQQQVAQAQQSQLPPQSQPQPQPMVSQPQAISGQIPSQVMPVTLTPQQLKAMQVRAQLVQQQQAAAAVQAAQAQAAQMGASGQVPILYSLLLLLILPFSTFQVSQNNITMMSSPSPVQQAQTPQSMPPPPQPQPSPQPGQPTSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPAAARTPQNFSVPSPGPLNTPGNPNSVMSPASSSQSEEQQYLEKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVPPTKQQYLCQPLLDAVLANIRSPVFNHSLYRTFMPAMTAIHGPPITAPVASPRKRKYEEDERQTIPNVLQGEVARLNPKFLVNLDPSHCSNNGTVHLICKLDDKNLPNVPPLQLSVPADYPDQSPLWIKNPRQYAANPFLQSVYRYMTSKLLQLPDKHSVTALLNTWAQSIRQACLSAA
Length769
PositionTail
OrganismAptenodytes forsteri (Emperor penguin)
KingdomMetazoa
LineageEukaryota> Metazoa> Chordata> Craniata> Vertebrata> Euteleostomi> Archelosauria> Archosauria> Dinosauria> Saurischia> Theropoda> Coelurosauria> Aves> Neognathae> Sphenisciformes> Spheniscidae> Aptenodytes.
Aromaticity0.04
Grand average of hydropathy-0.713
Instability index86.67
Isoelectric point9.59
Molecular weight85238.02
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU364148

GO - Cellular Component
integral component of membrane	GO:0016021	IEA:UniProtKB-KW
mediator complex	GO:0016592	IEA:InterPro

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP01366
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     313.58|      40|      40|     227|     266|       1
---------------------------------------------------------------------------
  151-  189 (63.27/10.68)	Q..QQQQFQQQ.QVALQQQQFQAQQsAMQ.QQFQV...QQQQAAAA
  224-  265 (75.64/14.58)	QqmQQQQQQQQLQRIAQMQQLQAAQ.AMQAQQQQQ...QQQQQQQQ
  266-  296 (53.45/ 7.58)	QipQQQIQQQPPQ...QV.........MQQQMQQM...QQQQQQQQ
  297-  334 (56.38/ 8.50)	Q..QQQQQQQ..Q.VAQAQQSQLPP...QSQPQPQpmvSQPQAISG
  403-  442 (64.84/11.17)	Q..VSQNNITMMSSPSPVQQAQTPQ.SMPPPPQPQ...PSPQPGQP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      72.13|      19|      23|     451|     473|       2
---------------------------------------------------------------------------
  454-  473 (35.49/21.58)	PAPSPSSFlPSPSPQPSQSP
  475-  493 (36.64/11.58)	AARTPQNF.SVPSPGPLNTP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      91.00|      23|      23|      84|     106|       3
---------------------------------------------------------------------------
   67-   94 (31.73/13.45)	PMNALQNLTG.GppagapGM.GMASRAQGA
   95-  116 (36.24/16.42)	PMSGMSGLGPMG........qQMSLPGQQQ
  117-  136 (23.04/ 7.73)	P.PGTSGMAPHG....mpGV.STATQ....
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      79.16|      24|     152|     193|     226|       4
---------------------------------------------------------------------------
  200-  223 (45.21/11.01)	QQLQAVQ......QQQQHMLKLQMQQQQNQ
  348-  377 (33.96/ 6.97)	QQLKAMQvraqlvQQQQAAAAVQAAQAQAA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.53|      14|      23|     663|     676|       5
---------------------------------------------------------------------------
  663-  676 (24.62/14.32)	VARLNPKFLVNLDP
  688-  701 (26.92/16.32)	ICKLDDKNLPNVPP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      91.18|      23|      23|     540|     562|       6
---------------------------------------------------------------------------
  518-  541 (29.91/17.41)	K......QLSKyIEPLRRMINKIDKNEDRK
  542-  564 (37.65/24.13)	K......DLSK.MKSLLDILTDPSKRCPLK
  565-  591 (23.62/11.93)	TlqkceiALEK.LKNDMAVPTPPPPPVP..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.57|      14|      23|     601|     614|       9
---------------------------------------------------------------------------
  601-  614 (23.65/16.53)	PLLDAVLA.NIRSPV
  625-  639 (21.91/14.73)	PAMTAIHGpPITAPV
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP01366 with Med15 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) AQAQQSQLPPQSQPQPQPMVSQPQAISGQIPSQVMPVTLTP
2) NLTGGPPAGAPGMGMASRAQGAPMSGMSGLGPMGQQMSLPGQQQPPGTSGMAPHGMPGVSTATQ
3) SQNNITMMSSPSPVQQAQTPQSMPPPPQPQPSPQPGQPTSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPAAARTPQNFSVPSPGPLNTPGNPNSVMSPASSSQSEEQQYLEKLKQ
307
73
405
347
136
519










Molecular Recognition Features






MoRF SequenceStartStop




NANANA