<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP01331

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDTYIDGRFERLEKALANLIDSVNKYHPSTVHAKELEAADEELTKGLEQIQAHQVNYLRVQQLRKSSAALDTQIKDTLTSLASTRRDITNTRTTTFPAGPNYPVAYDELLSYARRISKTTMPPAGTLKPPSGTSATTPEIQTPADMATPSASAAPTPSQPLSPAVNGASTPLPTQQAVVGATQQSAVTTNTSLPDVVSQYLNPLSGQLFFPWPLEDKIRNGALASNQILAEKGIDPRGYDPAEEEERQRKAEEEQKEREEQEKRELEERERRLREERERQRRERERQQEEWRKASMSGPSPERAPSRSNTGEKKQFQFRSLDDDLDEDDED
Length331
PositionMiddle
OrganismAcremonium chrysogenum (strain ATCC 11550 / CBS 779.69 / DSM 880 / JCM 23072 / IMI 49137)
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Hypocreomycetidae> Hypocreales> Hypocreales incertae sedis> Acremonium.
Aromaticity0.05
Grand average of hydropathy-1.047
Instability index57.75
Isoelectric point5.06
Molecular weight37218.54
Publications
PubMed=25291769

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP01331
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      47.35|      16|      30|     231|     257|       1
---------------------------------------------------------------------------
  230-  250 (21.27/23.09)	AEKGIDPRgydpaEEEERQRK
  257-  272 (26.07/ 6.09)	EREEQEKR.....ELEERERR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.36|      14|      16|     130|     143|       2
---------------------------------------------------------------------------
  130-  143 (26.56/13.58)	PSGTSATTPEIQ.TP
  149-  163 (21.80/ 9.87)	PSASAAPTPSQPlSP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      74.22|      21|      25|      81|     103|       3
---------------------------------------------------------------------------
   81-  103 (35.34/24.25)	LASTRRDItnTRTTTFPAGPNYP
  109-  129 (38.88/20.86)	LLSYARRI..SKTTMPPAGTLKP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      56.23|      17|      29|     276|     292|       4
---------------------------------------------------------------------------
  276-  292 (30.93/16.72)	ERERQRRER.ERQQEEWR
  302-  319 (25.30/12.46)	ERAPSRSNTgEKKQFQFR
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP01331 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) RNGALASNQILAEKGIDPRGYDPAEEEERQRKAEEEQKEREEQEKRELEERERRLREERERQRRERERQQEEWRKASMSGPSPERAPSRSNTGEKKQFQFRSLDDDLDEDDED
2) SKTTMPPAGTLKPPSGTSATTPEIQTPADMATPSASAAPTPSQPLSPAVNGASTPLPTQQAVVGATQQSAVTTNTSLPDVVSQY
219
117
331
200










Molecular Recognition Features






MoRF SequenceStartStop




1) AYDELL
2) EEWRKASMS
3) NTGEKKQFQFRSLDDDLDEDDED
105
289
309
110
297
331