<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP01287

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMDKIIDFRFERLEKALANLIDSVTKYHPSTAYAEELCSADDELSKGLEEVQTHQNNHLRIQELRKTSSALDAQIRQTLSTLASTRKDIVTTQTTEFPPGPSFPIAYEELLSYARRISKTTMPPSGTINGIPPVSESQTPIPESQPQSAITPTAMTPSQSQTQSPAPMNGLASQASEPPTQHTTTSVNTTLPEMMSQYLNPLSGQLFFPWPSEDKIRMGAMAMNQMLAERGIDPKGYDPVAEEERKVREELERKEREEREKLEREEREKKLWEERERLRLERERQREKEQEEWRRSSVAGGAAGGPGSSKPGATPGEKKQFQFTNLDDLDDDDD
Length333
PositionMiddle
OrganismStachybotrys chartarum IBT 40288
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Sordariomycetes> Hypocreomycetidae> Hypocreales> Stachybotryaceae> Stachybotrys.
Aromaticity0.05
Grand average of hydropathy-0.976
Instability index53.58
Isoelectric point4.98
Molecular weight37620.40
Publications
PubMed=25015739

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP01287
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      44.96|      14|      15|     251|     264|       1
---------------------------------------------------------------------------
  251-  264 (24.16/12.71)	ERKEREERE..KLERE
  267-  282 (20.80/10.00)	EKKLWEERErlRLERE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      96.53|      24|      24|     132|     155|       2
---------------------------------------------------------------------------
  117-  133 (24.43/ 8.49)	..SKTTMPPS..GTINGI.....PPV
  134-  157 (43.54/20.09)	SESQTPIPES..QPQSAITPTAMTPS
  159-  182 (28.56/11.00)	SQTQSPAPMNglASQASEPPTQHT..
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      71.69|      24|      30|     190|     216|       3
---------------------------------------------------------------------------
  190-  216 (37.91/34.11)	LPEMMSQY.LNPlSGqlFFPWPSED.KIR
  222-  247 (33.78/19.78)	MNQMLAERgIDP.KG..YDPVAEEErKVR
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP01287 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) PWPSEDKIRMGAMAMNQMLAERGIDPKGYDPVAEEERKVREELERKEREEREKLEREEREKKLWEERERLRLERERQREKEQEEWRRSSVAGGAAGGPGSSKPGATPGEKKQFQFTNLDDLDDDDD
2) TMPPSGTINGIPPVSESQTPIPESQPQSAITPTAMTPSQSQTQSPAPMNGLASQASEPPTQHTTTSVNTTLPEMMSQY
208
120
333
197










Molecular Recognition Features






MoRF SequenceStartStop




1) GSSKPGATPGEKKQFQFTNLDDLDDDDD
2) QEEWRRSSVAGGAAG
306
289
333
303