<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP01089

Description Mediator of RNA polymerase II transcription subunit 4
SequenceMLSVFQARFQRLETALNTLVESVAAYNPSVSAADALVAADEDLEESLGQLAVHHANYARILELRATADALDEKIKSTLRTLADTRKELLDIPSSQLAKNTRQVGVEELLSYAKFISKTTVPPTFRGHISTELLSKPQPVTADVPTTQITNGMATPAANGDNTSTSDQPEQTENRAVATLSAETKAMLDPLSQLPFVPWPSQDVIRMGALATIQGMIEDGTDPTTVLSAEEQEARDKQRQEEEERLQAEQEERERRRREDWAARGGGGRAAMTEDVFDPDEL
Length281
PositionMiddle
OrganismAureobasidium pullulans EXF-150
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Dothideomycetes> Dothideomycetidae> Dothideales> Saccotheciaceae> Aureobasidium.
Aromaticity0.04
Grand average of hydropathy-0.541
Instability index51.71
Isoelectric point4.61
Molecular weight30858.93
Publications
PubMed=24984952

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364141
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats
>MDP01089
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      48.03|      15|      17|     226|     241|       1
---------------------------------------------------------------------------
  226-  241 (21.50/16.39)	LSAeEQEARDKQRQEE
  245-  259 (26.53/15.56)	LQA.EQEERERRRRED
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      52.62|      16|      21|     107|     123|       2
---------------------------------------------------------------------------
  107-  123 (24.04/20.60)	ELLSYAKFISkTTVPPT
  131-  146 (28.58/19.30)	ELLSKPQPVT.ADVPTT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      79.87|      27|      30|      12|      41|       3
---------------------------------------------------------------------------
   12-   41 (39.14/31.14)	LETALNTLVESVAAYNPSV...SAADALvaaDE
   43-   72 (40.73/24.96)	LEESLGQLAVHHANYARILelrATADAL...DE
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      51.78|      16|      95|      80|      96|       4
---------------------------------------------------------------------------
   80-   96 (23.71/18.99)	TLADTRKELLDiPSSQL
  178-  193 (28.07/17.57)	TLSAETKAMLD.PLSQL
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP01089 with Med4 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) MGALATIQGMIEDGTDPTTVLSAEEQEARDKQRQEEEERLQAEQEERERRRREDWAARGGGGRAAMTEDVFDPDEL
2) RGHISTELLSKPQPVTADVPTTQITNGMATPAANGDNTSTSDQPEQTENRAVATLSAETK
206
125
281
184










Molecular Recognition Features






MoRF SequenceStartStop




1) EEEERLQAEQEERERRRREDWAARGGGGRAAMTEDVFDPDEL
240
281