<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP01000

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMATAELGQQTVELSTLVTRAAQDSYNSLKELVDKCRSTELSDSDKKISMLKFLTKTQQRMIRLNVLSKWCQQVPLIQHCQQLSSTVSNHDMCFTQAADSLFFMHEGLQQARAPVYDVPSAIEILLTGSYERLPKCIEDVGSQYALTEDKQKPALKKLDMLVRSKLLEVSLPKEISDIKVSDGTAMVRVNGEFQVLLTLGYRGHMSLWRILHLELLVGEKNKTVKLEELRRHVLGDDLERRMAAAENPFSILYTVLHELCVALVMDTVIRQVQFLRQGRWKDAIRFELISDGGSGHGASSSSVQNPDGESDSSGLRTPGLKIIYWLDFDKNAGVSDSGACPFIKIEPGPDLQIKCTHSIFVIDPLAGKEAEFFLDQNCIDVERLLLRAICCNRYTRLLEIKTELIKNVQVFRTADDVVLQSHMGEPDIEYKQKDNKRCDKDSEGNEVLHVRAYGSSFFTLGISIRNGRFLLQSSQNIVVSSALLECEEALNQGSITAAEVFLSLRSKSMLHLFASIGRVLGLEVYEHGLNTVKTPKTLSNGSTVLMMGFPDCGSSYFLLMQLDKNFKPLFKLLETEPDPSGKDKIFGDLNQVLRFKKIDIAQMQVLEDEMNLSLVDWAKLHSILPNAACPNQMSGHGLYSDTRLQNSMHTARGHHPSGFSSLVDDVFGLEKGSSAPPFPVQNISSPLNSSLPSHYGSLPINSQSLKAGNIHYNSSLFSSGNVKGPVQSSSVGSVPTGHGRSAVGKKLSASKSEQDLASVKSPHSVDISSSTPMDEDTANDALSGSRSSLLSPSWPINSRMSAPSSRPNGSSSCATTLVSQGLDTVNFSTSEDVISEQDKKSRKRTASDMLNLIPSLQEFVKNQGICKRRKILDACSSQLALPQSSITPEIIPKAEGCSYGSLIAEANKGNAPSSIYVAALLHVVRHSSLCIKHARLTSQMDALEISYVEEVGLRRVSSNIWFRLPFARGDSWQHIFLRLGRPGCMYWDVKIDDQHFRDLWELQKGSSNTSWGSGVRIANTSDIDSHIHYDPDGVVLSYQSVEKDSIKKLVADIQRLANARTFSIGMRKLLGIRADEKSEEFLTNSDVKISGVKTASDTADKLQMRRAFRIEAVGLMSLWFSFGSGVLARFVVEWESGKEGCTMHVSPDQLWPHTKFLEDFINGAEVSSLLDCIRLTAGPLHALAAATRPARAGPVPGVAAIISSFPKQTGYTSSQGLLLGSSTSATNVGQPASGLGANTSVSNASGISNQNLSMLAAAGRTGPGIVPSSLLPFDVSVVLRGPYWIRIMYRKQFAVDMRCFAGDQVWLQPATPPKEGRPSGGSLPCPQFRPFIMEHVAQELNGLDPSFTGQQAGGLTSSNSPNPNSGTQSMAANGNRINSAAMSRTGNQVASLNRMGNALAGSSNLALMTSAVPLRRPPGTVVPAHVRGELNTAIIGLGDDGGYGGGWVPLVALKKVLRGILKYLGVLWLFAQLPDLLKEILGSILKDNEGALLNLDPEQPALRFFVGGYVFAVSVHRVQLLLQVLSVKRFHHQQQQQQQNSNPAPEELSPSEISEICDYFSRRVASEPYDASRVASFITMLTLPIPVLREFLKLIAWKKGLSQAQVGDVVSAQKPRIELCLENHAGLNADENSESSSAFRSNIHYDRLHNSVDFALTVVLDSAHIPHVNAAGGAAWLPYCVSVRLRYSFGESLNVSFLGMSGSHGGRACWSRVDDWEKCKQRVARTVEVSASSAADVSQGRLKLVADSVQRNLHMCIQGLRDGSGATASSGAT
Length1772
PositionTail
OrganismMedicago truncatula (Barrel medic) (Medicago tribuloides)
KingdomViridiplantae
LineageEukaryota> Viridiplantae> Streptophyta> Embryophyta> Tracheophyta> Spermatophyta> Magnoliopsida> eudicotyledons> Gunneridae> Pentapetalae> rosids> fabids> Fabales> Fabaceae> Papilionoideae> 50 kb inversion clade> NPAAA clade> Hologalegina> IRL clade> Trifolieae> Medicago.
Aromaticity0.07
Grand average of hydropathy-0.177
Instability index46.81
Isoelectric point7.83
Molecular weight193349.96
Publications
PubMed=22089132
PubMed=24767513
PubMed=30397259

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
core mediator complex	GO:0070847	IBA:GO_Central
mediator complex	GO:0016592	IBA:GO_Central
GO - Biological Function
transcription coregulator activity	GO:0003712	IBA:GO_Central
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IBA:GO_Central

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP01000
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     118.76|      38|     564|      89|     133|       1
---------------------------------------------------------------------------
   21-   61 (53.79/29.05)	AQDSYNSLKELVDKCRStELSDSDKKISMLkfLTKTQQRMI
   96-  133 (64.97/31.24)	AADSLFFMHEGLQQARA.PVYDVPSAIEIL..LTGSYERLP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      31.08|      10|      23|     698|     709|       2
---------------------------------------------------------------------------
  698-  709 (13.59/10.68)	PINSQSLkaGNI
  724-  733 (17.50/ 7.38)	PVQSSSV..GSV
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     587.34|     172|     693|     891|    1073|       3
---------------------------------------------------------------------------
  891- 1073 (280.43/259.46)	PKAEGCSYGSLIAEANKGNAPSSIYVAALLHVVRHSSLCIKHARLTSQ.MDALEIS...YVEEVGLrrvsSNIWF......RLPFARGDSWQHIF.LRL....GRPGCMYWDVKIDD..QHFRDLWELQKGSSNTSWGSGVRIantsDIDSHIHYDPDGVVLSYQSVEKDSIkKLVAD.IQRlaNARTFSIGMRKLLGIRA
 1081- 1174 (82.45/56.82)	................................LTNSDVKISGVKTASDtADKLQMRrafRIEAVGL....MSLWF........SFGSGVLARFVVeWES....GKEGCTM.HVSPDQlwPHTKFLEDFINGAEVSSLLDCIRL..........................................................
 1614- 1767 (224.47/179.38)	PRIELCLENHAGLNADENSESSSAFRSNIHYDRLHNSV...DFALTVV.LDSAHIP...HVNAAG.....GAAWLpycvsvRLRYSFGESLNVSF.LGMsgshGGRAC..WS.RVDD........WE....................KCKQRVARTVEVSASSAADVSQGRL.KLVADsVQR..NLHMCIQGLRDGSGATA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      87.11|      25|     693|     643|     691|       4
---------------------------------------------------------------------------
  667-  691 (45.66/53.83)	GLEKGSSAP.....PFPVQNISSPLNSSLP
 1315- 1344 (41.44/10.81)	GRPSGGSLPcpqfrPFIMEHVAQELNGLDP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      67.42|      19|      96|     441|     459|       5
---------------------------------------------------------------------------
  441-  459 (35.03/24.20)	SEGNEVLHV..RAYGSSFFTL
  538-  558 (32.40/21.74)	SNGSTVLMMgfPDCGSSYFLL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      44.76|      14|     693|     781|     794|       7
---------------------------------------------------------------------------
  746-  761 (17.93/ 6.99)	LSASKSeqDLASVKSP
  781-  794 (26.83/14.63)	LSGSRS..SLLSPSWP
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     148.28|      48|     730|     829|     877|       8
---------------------------------------------------------------------------
  829-  877 (77.71/53.21)	SEDVISEQDKKSRKRTASDMLNL.IPSLQEFVK....NQGIcKRRKILDACSSQ
 1560- 1612 (70.57/42.78)	SRRVASEPYDASRVASFITMLTLpIPVLREFLKliawKKGL.SQAQVGDVVSAQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      42.33|      12|     338|     293|     304|      10
---------------------------------------------------------------------------
  293-  304 (22.59/12.55)	SGHG.ASSSSVQN
  633-  645 (19.75/ 9.94)	SGHGlYSDTRLQN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      61.65|      17|     115|    1228|    1245|      11
---------------------------------------------------------------------------
 1228- 1244 (30.43/10.48)	GQPASGLGANTSVSNAS
 1348- 1364 (31.22/11.02)	GQQAGGLTSSNSPNPNS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      73.82|      22|     153|    1245|    1266|      18
---------------------------------------------------------------------------
 1245- 1266 (40.15/26.59)	GISNQNLSMLAAAGRTGPG.IVP
 1400- 1422 (33.67/20.89)	GSSNLALMTSAVPLRRPPGtVVP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP01000 with Med14 domain of Kingdom Viridiplantae


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) GLDPSFTGQQAGGLTSSNSPNPNSGTQSMAANGNRIN
2) KGPVQSSSVGSVPTGHGRSAVGKKLSASKSEQDLASVKSPHSVDISSSTPMDEDTANDALSGSRSSLL
1341
722
1377
789










Molecular Recognition Features






MoRF SequenceStartStop




1) LKIIYW
319
324