<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP00950

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMNGHRPPTNGEYHMSNGIQRDGASSASQDIAPKGERLPQKPSVAFSDHEQGHTNGRPSDIPLRHSTPPHAPASRSQQPPLEILQLISKDSYLPLAALVGRSAQSCWNSLSILLEELASIHVSDHVGESSKPPPSGQTNNQSKANLEKKDKLLQFANEQKANFIKLLVLLQWSRDVDDVSKTISINYWLMTRRQAYWDVIASLALIKQDSAGFQVPNPDLKTAAEILSKGKTVEMPTLGYIPQRDLSSKQMLHVLKSLNQLLSIRLAMSDNLPPPLQNFHIHDGRATFVIPNEFEMDVSVLDNSPGSQFRMVDFRFAFTPAPSISDGMRSEIENFANSNIERDGLPGCYLFLQELILSYKLAEHYKQAIELTRTQWAGNLRVELIRRTLVVQYWSKRQTAKSWVEIGIASGHDKSQNSENLPHLDVRWIRYGQLIASPMLSTTQSVLNLEEILRQVVAQDSTLLLDDIYDNLNSTPLFANGDLSLEQSISATCPEECALTIQLSHSSQIDLGIDWVTGSFVISPVTERSERLQYEINRAHGVGGEIVSKLLNFRCSVSESAISAAMLATNWEVLRTFRFSQSEIKSLFGGPVARLNSFRKPRWSSDYFVTISHTHNGDQWWLMHRTKFIGSSGQGQFQVVRSQQMKVTHKLSPAYFDRLSEYTTGVITLRRNAEYLKERNHTHDLPPFPDFEDGYKLPELSFRLTGSQQTSSILDTKSKDDDHRTMVNVRFGGVDPATNMTTVIGKIHLDGNAGISKYLDKSTLDPDVSLNFSDQSATIRVETSITEAAIPTILDKAVHLKNMISTVQEIRRLPGITLKSVSKSGFSVYYYSAPSKELSVQVSFRDSGQAPQLMFFPREENPHSLLAEKYSDTLVFDQHPFEIRIHNFLTSLIMTYPLALYLHNLQKNYGQAVKTDSGLTSTETGPQLRVHILVRTAQHFAIQYFTNKNHYSKDGAPESQTSLVARLEILPHVDHGSKKPMWLVRAAMEEFQAYSRPSFSSTSLRLKLRQGVFSRTETPTKWLTLDTAAACLADNPEPLLQEIHNLITNFVNEQSTTQVGEVAPAKADAQKASTGGVSNGNIKSKGQPRAAGKMQAPAAIPNGAVKPPPQRVNNPAGRAFPGKSQMKSNAPNNQDNPITLD
Length1140
PositionTail
OrganismExophiala aquamarina CBS 119918
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Chaetothyriomycetidae> Chaetothyriales> Herpotrichiellaceae> Exophiala.
Aromaticity0.08
Grand average of hydropathy-0.415
Instability index47.70
Isoelectric point8.01
Molecular weight127121.21
Publications









Function






Annotated function

Component of the Mediator complex, a coactivator involved in
the regulated transcription of nearly all RNA polymerase II-dependent
genes. Mediator functions as a bridge to convey information from gene-
specific regulatory proteins to the basal RNA polymerase II
transcription machinery. Mediator is recruited to promoters by direct
interactions with regulatory proteins and serves as a scaffold for the
assembly of a functional preinitiation complex with RNA polymerase II
and the general transcription factors. 


ECO:0000256	RuleBase:RU365082

GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule

GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule

GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro









Interaction





Binary Interactions











Repeat regions





Repeats

>MDP00950
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      50.33|      15|      15|     139|     153|       1
---------------------------------------------------------------------------
  139-  153 (25.19/19.11)	NQSKANLEKKDKLLQ
  156-  170 (25.14/19.05)	NEQKANFIKLLVLLQ
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     408.74|     136|     217|     558|     713|       2
---------------------------------------------------------------------------
  558-  713 (205.36/174.31)	ESAISAAMLAT..NWEV.LRTFRFSQSEIKSLfggPVARLNSFRKPRWSSDYFVTISHThngdqwwLMHRTKFiGSSGQGQfQVVRSQQMKVTHK.LSPAY.....FD......RLSEYTTGVITLRRNAEYLkernhtHDLPpfPDFEDGYKLPE.LSFRLTGSQQTSSIL
  781-  932 (203.38/123.42)	ETSITEAAIPTilDKAVhLKNMISTVQEIRRL...PGITLKSVSKSGFSVYYYSAPSKE.......LSVQVSF.RDSGQAP.QLMFFPREENPHSlLAEKYsdtlvFDqhpfeiRIHNFLTSLIMTYPLALYL......HNLQ..KNYGQAVKTDSgLTSTETGPQLRVHIL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     422.19|     141|     199|     171|     332|       3
---------------------------------------------------------------------------
  171-  332 (226.73/204.92)	WSRD..VDDVSKTISINYWLMTRRQAYWDVIASLALIKQDSAGFQVPNPDLKtaaeilskgktvemptlgYIPQRDLSSKQMLHVLKSLNQLLSI.RLAMS...........DNL.PPPL.QNFHIH.DGRATFVIPNEFEMDVSvLDNSpgSQFRM.VDF...RFAFTPAPSISDGMRSEIE
  375-  536 (195.46/137.70)	WAGNlrVELIRRTLVVQYWSKRQTAKSWVEIGIASGHDKSQNSENLPHLDVR..................WIRYGQLIASPMLSTTQSVLNLEEIlRQVVAqdstlllddiyDNLnSTPLfANGDLSlEQSISATCPEECALTIQ.LSHS..SQIDLgIDWvtgSFVISPVTERSERLQYEIN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     304.73|      99|     924|      16|     129|       4
---------------------------------------------------------------------------
   16-  129 (149.34/108.38)	NGIQRDGASSASQDIAPKGERLP......QKP.SVAFSDHEQGHTNGRPSdiplrhstpphaPASRSQQppLEILQLISKDSYLPLAALVGRSAQSCW.NSLSILLEELASIhVSDHVGESS
  948- 1054 (155.39/82.66)	NHYSKDGAPESQTSLVARLEILPhvdhgsKKPmWLVRAAMEEFQAYSRPS............FSSTSLR..LKLRQGVFSRTETPTKWLTLDTAAACLaDNPEPLLQEIHNL.ITNFVNEQS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      88.51|      20|      27|    1087|    1106|       5
---------------------------------------------------------------------------
 1063- 1083 (25.48/14.75)	P..AK.ADAQKAsTGGVSNGNIKS
 1087- 1106 (36.35/24.55)	P..RA.AGKMQA.PAAIPNGAVKP
 1114- 1136 (26.68/15.83)	PagRAfPGKSQM.KSNAPNNQDNP
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP00950 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) FVNEQSTTQVGEVAPAKADAQKASTGGVSNGNIKSKGQPRAAGKMQAPAAIPNGAVKPPPQRVNNPAGRAFPGKSQMKSNAPNNQDNPITLD
2) MNGHRPPTNGEYHMSNGIQRDGASSASQDIAPKGERLPQKPSVAFSDHEQGHTNGRPSDIPLRHSTPPHAPASRSQQPPL
1049
1
1140
80










Molecular Recognition Features






MoRF SequenceStartStop




1) KGQPRAAGKMQAPAAIPNGAVKPPPQRVNNPAGRAFPGK
2) QDIAPKGERLPQKPSVAF
1084
28
1122
45