<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP00832

Description Mediator of RNA polymerase II transcription subunit 1
SequenceMDKTKEWQMELLMEKLRSKAGQFKSFVESAKALRMSILEKRYPVDNVERNQLQKCLDILQHSIKVTSLQGMVERLESVTRQLGLKFSPGGSPLDWFISSDMFYLEVMLESTGGVKDVRIHHEGKVEQQSCEELVSCLSRGDFADFTAQLEGLASIYQLNAEKKVKCKAFSALQSLEADLGTLATLQSFIKEPFNLVHKSPVGILEKRRGGHAMKLTYLVPPYDLLDQQTNSSLSLSVEEVTSRNLGYSVMVCLEGSAAHKLQTTALISVNRSPSGKSTPLYASLSAANSATLPACFVLRLNNPMPMCLNLVRRIQQVTEMECGDLSTPHPLLSLVTQHASAGQLDCANNRGLFVTLPDQQHCFFMTESKNLEGILVSNIPFLHPAHVPQILMLLRQQALFNVLVSSCIRPASKHADLESVIMFEVTALSWQHLSISFEHPLEETMTTAELDLADISNIKCKVYGAGNGENGCTPEHASKVLQRCWSIPVTMRSLIRLWQSHNLRSNGLTAGGNLSLPPGSDPGRGHGGSMTPDFDVSRVKAEPDGSSLPHSQSLSGSGSRQGFGPDMSDTGVGFQSPGNPIESSVSNTFQFGALLSSQGSVGKGPVLLNMLSEQTPNTKKQRKRNNKDGMWRSPKRKQSEECEIVLESSSSDSTPLGTPTSRDAPMETILDCEEEKTGSDFGEEEIMPVEEVEEMIVRKPKKEKKTSPPTIILDLAESKNLVAPSVSITPITSSSSSFNSVLTGMGLERRPGIEIIPIVSTPATSLHSSITITPISSKVIAADDRNYRDRKSGKSRTEDKMRPDKKRNKRKREDSPSGCGGSSVAMGPPDKLPPKQDPLSKPVSVSIKPTESPPMTSVRPSSPASALRKVTSSPTHVNLSLTKSSSSKPSSSHQSPKHSPGYTTSSPKHHGTSSPKHQSSGGSGKPSMSALKSAASSPSTAGKSGSDVPKVKSSSSKDKDRKVSSSYSGASSGSGSSPKLKSSSVKLKQLDITSGSGDGGTGPQSGAGTPPLVTIDGNKSSAVPQVRNRKGSLSAVIDKLKSAQHQGPEPGEVMKEGRGSGTNPSSKIPGDTSGSIISSSKNPGEYMVKSSSEGIKITINKTRTKDSKSGVVKTSSSSSSSTGSGSPKTHTGLKPGVNSGPASKKPHQMLQQKSTSGNSGSSGISSSSSKAGSGSSSGNPSSKIPSTIKSTSSPVSGILSKSASKSSGSPKMSSLADANRRDNKPRTLKTGSDREKPVFSKGPDARKSSPIGLRDELEGERALKLVTAHAKIEPSLPPQLIVEGMIKSLDTKFQIPKLSARSSNLNEGDSKKQSSDKTSIGGTESSNRMDSGAKPIDLVGKVDGSNTSTKFPPLSKTDDMKGSKIPALASVTPALAISSLTTPTLKPPPTTAFPSVTSKLQDINLDTCPQISAPTAAPIVASMSSSSSEPTSGETLEVSRMKTDLPRSGQSSGNNAKEETTIQVIGKLENPVKSFSASNNNNDPSVLSTLHLSKTVDSSQTKFSSSSSQKQAEDSKKLVNLSDIPNKSTGLNKMTPAPTTSQEAAEILLDMSASSSLQNKPLVVEQQAGKLATVPERAVAVSAPSRRNTPPPPLPPPQPSVQVHILQSPAAAASTTASPVVITSPHSASPCSIDDELMDEALVGMGK
Length1647
PositionMiddle
OrganismZootermopsis nevadensis (Dampwood termite)
KingdomMetazoa
LineageEukaryota> Metazoa> Ecdysozoa> Arthropoda> Hexapoda> Insecta> Pterygota> Neoptera> Polyneoptera> Dictyoptera> Blattodea> Blattoidea> Termitoidae> Termopsidae> Zootermopsis.
Aromaticity0.03
Grand average of hydropathy-0.503
Instability index57.96
Isoelectric point9.31
Molecular weight174360.88
Publications
PubMed=24845553

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	RuleBase:RU364059
GO - Cellular Component
mediator complex	GO:0016592	IEA:InterPro
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:InterPro
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP00832
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      65.45|      25|      26|     933|     957|       1
---------------------------------------------------------------------------
  888-  913 (30.39/ 8.30)	KPSSSHQSPKHSPGYTTSSPK.........hHGTS
  937-  971 (35.06/10.92)	SPSTAGKSGSDVPKVKSSSSKdkdrkvsssySGAS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             4|     123.15|      24|      24|    1352|    1375|       2
---------------------------------------------------------------------------
 1277- 1305 (28.68/13.20)	PPqlivegMIKSLDTK.FQIPKLSARSSNL
 1352- 1375 (40.51/22.24)	PP......LSKTDDMKGSKIPALASVTPAL
 1378- 1400 (34.13/17.36)	SS......LT.TPTLKPPPTTAFPSVTSKL
 1401- 1417 (19.83/ 6.44)	Q............DINLDTCPQISAPTAA.
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             5|     250.39|      55|      56|    1006|    1060|       3
---------------------------------------------------------------------------
  853-  887 (29.23/ 6.22)	....P.PMTS..V..RPSSPASA.LRKVTSSPTHVnlsLTKSSSS..............................
 1006- 1060 (86.91/33.95)	GAGTP.PLVT..IDGNKSSAVPQ.VRNRKGSLSAV...IDKLKSAQ..........HQGPEPG...EVMKEGRGS
 1096- 1143 (40.34/11.56)	.......KIT..I..NKT.......RT.KDSKSGV...VKTSSSSSsstgsgspktHTGLKPG.....VNSGPAS
 1178- 1222 (49.40/15.92)	..............GNPSSKIPStIKSTSSPVSGI...LSKSASKS..........SGSPKMS...SLADANRRD
 1231- 1284 (44.51/13.57)	GSDREkPVFSkgPDARKSSPI.G.LRDELEGERAL.....KLVTA...........HAKIEPSlppQLIVEG...
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      90.06|      27|      27|     110|     136|       4
---------------------------------------------------------------------------
  110-  136 (48.16/25.99)	STGGVKDVRIHHEG..KVEQQSCEELVSC
  138-  166 (41.90/21.83)	SRGDFADFTAQLEGlaSIYQLNAEKKVKC
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      65.92|      23|      27|    1452|    1478|       5
---------------------------------------------------------------------------
 1452- 1478 (32.73/29.30)	SGNNAKEETTIQvigkLENPVKS....FSAS
 1480- 1506 (33.20/19.28)	NNNDPSVLSTLH....LSKTVDSsqtkFSSS
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      80.53|      26|      27|     724|     750|       6
---------------------------------------------------------------------------
   62-   87 (38.18/16.32)	SIKVTSLQGMVERLESVTRQLGLKFS
  725-  750 (42.35/24.72)	SVSITPITSSSSSFNSVLTGMGLERR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      81.74|      24|      27|     300|     324|       7
---------------------------------------------------------------------------
  300-  324 (39.74/32.61)	LNNPMPMcLNLVRRIQQVTEMECGD
  325-  348 (42.00/28.02)	LSTPHPL.LSLVTQHASAGQLDCAN
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     107.91|      25|      27|     783|     809|       8
---------------------------------------------------------------------------
  521-  538 (28.09/ 7.22)	......DPGRGHGGS...M.TPDFDVSR
  783-  809 (35.06/15.44)	DDRNyrDRKSGKSRTEDKM.RPDKKRNK
  810-  835 (44.77/16.04)	RKRE..DSPSGCGGSSVAMgPPDKLPPK
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      77.98|      25|      27|     563|     588|       9
---------------------------------------------------------------------------
  563-  588 (42.90/23.19)	FGPDMSDTG.VGfQSP..GNPIESSVSNT
  591-  618 (35.08/14.26)	FGALLSSQGsVG.KGPvlLNMLSEQTPNT
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|      90.31|      23|      27|     371|     393|      10
---------------------------------------------------------------------------
  371-  393 (40.98/28.21)	LEGILVSNI..PFLHPAHVPQILML
  399-  423 (32.70/20.74)	LFNVLVSSCirPASKHADLESVIMF
  428-  440 (16.63/ 6.24)	LSWQHLS.I..SFEHP.........
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      87.69|      26|      28|     170|     195|      12
---------------------------------------------------------------------------
  170-  195 (41.66/27.01)	SALQSLEADLGTLATLQSFIKEPFNL
  199-  224 (46.02/30.65)	SPVGILEKRRGGHAMKLTYLVPPYDL
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      32.44|      10|      28|     641|     652|      13
---------------------------------------------------------------------------
  641-  652 (13.30/11.60)	ECEivLESSSSD
  671-  680 (19.14/ 9.73)	DCE..EEKTGSD
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      90.83|      28|      60|    1536|    1564|      15
---------------------------------------------------------------------------
 1536- 1564 (42.04/28.69)	PAPTTSQEaAEILLDMSASSSLQNKPLVV
 1595- 1622 (48.79/29.35)	PPPQPSVQ.VHILQSPAAAASTTASPVVI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      45.56|      15|     341|     973|     999|      16
---------------------------------------------------------------------------
  973-  990 (19.94/ 6.08)	GSGSSPKLKSSSvklKQL
 1322- 1336 (25.62/14.69)	GTESSNRMDSGA...KPI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      66.06|      21|      30|     235|     258|      17
---------------------------------------------------------------------------
  235-  258 (30.43/21.30)	LSVEEVTSrnlGYSVMVCLEGSAA
  267-  287 (35.63/17.61)	ISVNRSPS...GKSTPLYASLSAA
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.00|      13|      61|    1081|    1094|      20
---------------------------------------------------------------------------
 1062- 1074 (23.06/12.79)	TNPSS.KIPGDTSG
 1081- 1094 (17.94/14.62)	KNPGEyMVKSSSEG
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP00832 with Med1 domain of Kingdom Metazoa


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) GKGPVLLNMLSEQTPNTKKQRKRNNKDGMWRSPKRKQSEECEIVLESSSSDSTPLGTPTSRDAPMETILDCEEEKTGSDFGEEEIMPVEEVEEMIVRKPKKEKKTSPPTIILDLAESK
2) INLDTCPQISAPTAAPIVASMSSSSSEPTSGETLEVSRMKTDLPRSGQSSGNNAKEETTIQVIGKLENPVKSFSASNNNNDPSVLSTLHLSKTVDSSQTKFSSSSSQKQAEDSKKLVNLSDIPNKSTGLNKMTPAPTTSQEAAEILLDMS
3) IPKLSARSSNLNEGDSKKQSSDKTSIGGTESSNRMDSGAKPIDLVGKVDGSNTSTKFPPLSKTDDMKGSKIPAL
4) LTAGGNLSLPPGSDPGRGHGGSMTPDFDVSRVKAEPDGSSLPHSQSLSGSGSRQGFGPDMSDTGVGFQSPGNPIESSVSNTFQ
5) SKVIAADDRNYRDRKSGKSRTEDKMRPDKKRNKRKREDSPSGCGGSSVAMGPPDKLPPKQDPLSKPVSVSIKPTESPPMTSVRPSSPASALRKVTSSPTHVNLSLTKSSSSKPSSSHQSPKHSPGYTTSSPKHHGTSSPKHQSSGGSGKPSMSALKSAASSPSTAGKSGSDVPKVKSSSSKDKDRKVSSSYSGASSGSGSSPKLKSSSVKLKQLDITSGSGDGGTGPQSGAGTPPLVTIDGNKSSAVPQVRNRKGSLSAVIDKLKSAQHQGPEPGEVMKEGRGSGTNPSSKIPGDTSGSIISSSKNPGEYMVKSSSEGIKITINKTRTKDSKSGVVKTSSSSSSSTGSGSPKTHTGLKPGVNSGPASKKPHQMLQQKSTSGNSGSSGISSSSSKAGSGSSSGNPSSKIPSTIKSTSSPVSGILSKSASKSSGSPKMSSLADANRRDNKPRTLKTGSDREKPVFSKGPDARKSSPIGLRDELEGERALKLVTAHAKIEPSLPPQLI
6) STTASPVVITSPHSASPCSIDDELMDEALVGMG
7) TVPERAVAVSAPSRRNTPPPPLPPPQPSVQVHILQSPA
602
1403
1295
508
777
1614
1573
719
1552
1368
590
1281
1646
1610










Molecular Recognition Features






MoRF SequenceStartStop




1) IGLRDELEGERALKLV
2) PKLKSSSVKLKQLDITS
3) SKKPHQMLQ
4) SLSAVIDKLKSAQ
5) VHILQSPAAAASTTAS
1251
978
1143
1032
1603
1266
994
1151
1044
1618