<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Detailed information on MDP00100

Description Mediator of RNA polymerase II transcription subunit 14
SequenceMGNVNVGGAGQEPGIHETKNGLSNPGGPGNSTELRNGTTHVNGVSGTASGQLWKTEASVASGEPPALVHITQGFFPFSMLVNRSVQQCWNDLSELVAELAEMQVPYQDQSSLSFTPHGKSSGNQTSANVQKKLRLLDFAHAKRAEFIKLLVLSQWSRQAADVSQLIDLQGFIRTRHQAYAAALQWVGDMKRDLVMAQVANPDLKTALEVISKGRVMAMSDLGYKPPKPLTAKRTLKKLQKLNKIISIRLTLHDQVPRAFQTYHVHDGRVTFSVPGEFELDLSVGEENKSSQFFFVDIRFLFSPSSAIPKGRVLNELDIRINDVLRDSGLTGCFDLLHGLVLTFKVNVLFKQAIDLARGLWSDVLRVELLHRTLIVQYWALKPGAKSWLEVGIKSGRLRGSHGDPGVPCLGLRWMRDGQEVDNANIEFDTQILSMEYVLRSVIALHVSHILSSVYTNICENSLFSTGSLSLCAQLSRMEPGDCQLDIQLTSSRHLRVTVEPMSGASVLSATPSILDRFDSDRGSDKSLTDEIASRVARIRCSSAIEEIESNLKILGFETVNPRSLKVDIRRIFPSNIIRFTFFRHHLWEHNWLIAATSSMDSDNWWVIQLRPTLSANKHPNLGARDASMLRSAQVISSRFLTAQQHTSYAACADLGHCLTGILAIHANARFLVELQCIHFHPPLHKLQIETGLRTPDIFIRYEASNLPPALRIAQPAGLKKSYIKNTIRVAFHGIDPHKNLAILVAYGSLSMPVKALSTLVSKWDPSIVFRQKGGGFAIRLLAPAGQIVIVDLIEKLQRLEQILSIIESLQRKKMDVLSLSLSRIAFSYGPGKVLRANINVSTSGPSSSAHTDLSGIPSKADSVFLQRLGIVFDHPNPHRRIQGSLTTSLNNASSEAGLDSFFELLTLTLPLLRALDQITVNPSHNEPLKAQVTVRNAKTYQIHYPIAKFRFQLVAGQHLNRMTWILKDVSGTQDRSHQSQITGRLREKLYNANGDGWRGLGTGIVADVDKVSNLVLELDGCFSDSRSTIATPGHSAVGHGTRNVGQKQAVGQGDPRQIINAGVRPSGVVSAVGAPGMKPEQKASEDIIMID
Length1091
PositionTail
OrganismAspergillus ruber CBS 135680
KingdomFungi
LineageEukaryota> Fungi> Dikarya> Ascomycota> Pezizomycotina> Eurotiomycetes> Eurotiomycetidae> Eurotiales> Aspergillaceae> Aspergillus.
Aromaticity0.07
Grand average of hydropathy-0.129
Instability index39.83
Isoelectric point9.36
Molecular weight120067.18
Publications
PubMed=24811710

Function

Annotated function Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 
ECO:0000256	ARBA:ARBA00003669
ECO:0000256	RuleBase:RU365082
GO - Cellular Component
mediator complex	GO:0016592	IEA:UniProtKB-UniRule
GO - Biological Function
transcription coregulator activity	GO:0003712	IEA:UniProtKB-UniRule
GO - Biological Process
regulation of transcription by RNA polymerase II	GO:0006357	IEA:InterPro

Interaction

Binary Interactions











Repeat regions





Repeats

>MDP00100
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      34.06|      11|      15|     521|     533|       1
---------------------------------------------------------------------------
  521-  533 (14.18/17.20)	RGSdkSLTDEIAS
  539-  549 (19.88/14.15)	RCS..SAIEEIES
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.27|      17|      23|     681|     697|       3
---------------------------------------------------------------------------
  681-  697 (30.66/18.53)	PPLHKLQIETGLRTPDI
  707-  723 (29.61/17.66)	PPALRIAQPAGLKKSYI
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      60.67|      20|      94|     373|     398|       4
---------------------------------------------------------------------------
  348-  367 (34.11/14.61)	LFKQAIDLARGL..WSDV....LRVE
  373-  398 (26.56/22.39)	LIVQYWALKPGAksWLEVgiksGRLR
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|      41.95|      12|      16|       7|      21|       5
---------------------------------------------------------------------------
    7-   21 (18.29/24.51)	GGAGQEPgihETKNG
   26-   37 (23.66/17.47)	GGPGNST...ELRNG
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             3|     256.44|      63|     647|     274|     337|       7
---------------------------------------------------------------------------
  274-  337 (103.95/80.79)	PGEFE.LDLSVGEEN.KSSQFFFVDIRFLFSPSSaIPKGRVLNELDIRINDVLRDSGLTGCFDLLH
  852-  905 (72.53/50.38)	.......DLS.GIPS.KADSVFLQRLGIVFDHPN..PHRRIQGSLTTSLNNASSEAGLDSFFELL.
  922-  977 (79.95/56.43)	PSHNEpLKAQVTVRNaKTYQIHYPIAKFRFQ....LVAGQHLN....RMTWILKD..VSGTQDRSH
---------------------------------------------------------------------------
---------------------------------------------------------------------------
No. of Repeats|Total Score|Length  |Diagonal| BW-From|   BW-To|   Level
             2|     259.76|      82|     120|     430|     517|       9
---------------------------------------------------------------------------
  430-  517 (124.34/92.75)	QILSMEYVLRSVIALHVSHILSSvytNICENSLFSTGSLS...LCAQLSRMEpGDCQLDIQLTSSrhLRVTVEPMSG...ASVLSATPSILDRF
  552-  639 (135.42/82.10)	KILGFETVNPRSLKVDIRRIFPS...NIIRFTFFRHHLWEhnwLIAATSSMD.SDNWWVIQLRPT..LSANKHPNLGardASMLRSAQVISSRF
---------------------------------------------------------------------------




































Explaination for Stockholm format
  The  "Stockholm" format is a system for marking up features in a multiple alignment. These mark-up annotations are preceded by a 'magic' label, of which there are four types. The Stockholm format is used by HMMER, Pfam, and Belvu. Mark-up lines include any characters except whitespace. Underscore ("_") is used instead of space. 

 #=GR (seqname) PP (Generic per-Sequence AND per-Column markup, exactly 1 char per column) where PP is Posterior Probability  [0-9*], (0=0.00-0.05; 1=0.05-0.15; *=0.95-1.00) 
 
 #=GC PP_cons line is Stockholm-format consensus posterior probability annotation for the entire column.  It’s calculated simply as the arithmetic mean of the per-residue posterior probabilities in that column.  This should prove useful in phylogenetic inference applications, for example, where it’s common to mask away non confidently aligned columns of a multiple alignment. The PP_cons line provides an objective measure of the confidence assigned to each column.
 
 #=GC RF line is Stockholm-format reference coordinate annotation, with an x marking each column that the profile considered to be consensus.





Alignment of MDP00100 with Med14 domain of Kingdom Fungi


     
       
      
        
        
        


      
      
   
   

      

      














Intrinsically Disordered Regions








IDR SequenceStartStop




1) IATPGHSAVGHGTRNVGQKQAVGQGDPRQIINAG
2) MGNVNVGGAGQEPGIHETKNGLSNPGGPGNSTELRNGTTHVNGVSGTA
1029
1
1062
48










Molecular Recognition Features






MoRF SequenceStartStop




NANANA