Welcome to tncRNA Toolkit

Transfer RNA-derived small ncRNAs(tncRNAs) are 14-50 nucleotide length tRNA fragments responsible for the gene regulation at both transcription and translation levels. tncRNAs can be broadly classified into tRF-5, tRF-3(CCA), tRF-1, 5' tRH and 3' tRH(CCA), leader-tRF and other-tRF which are named according to the contributing portion of tRNA segment.
tncRNA Toolkit is a pipeline, designed for convenient identification of tncRNAs. It identifies the tncRNAs using small RNA sequencing datasets. It also provides detailed information related to each tncRNA entry viz. tncRNA type, tRNA information, position on tRNA, strand, sequence, length, read count, RPM and modification site.

Get tncRNA Toolkit from here: DOWNLOAD

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tncRNA Toolkit Features

• Identification of tncRNA- tncRNA Toolkit detects tRNA-derived ncRNAs viz. tRF-5, tRF-3(CCA), tRF-1, 5' tRH, 3' tRH(CCA), leader-tRF and other-tRF using small RNA sequences provided by the User. The identification of tncRNAs is done on the basis of origin and site of cleavage of mature tRNAs or precursor tRNAs.

• tncRNAs attributes- This pipeline gives following information related to each identified tncRNA viz. tncRNA type, frequency, small RNA read length, tRNA information (amino acid, anticodon, locus), Modification sites, tncRNA locus (on tRNA), tncRNA sequence, and length.

• tncRNA Alignment- Output file "Alignment.txt" shows the local alignment of tncRNA on their respective origin.


If you are using this toolkit, please cite: Transfer RNA-derived non-coding RNAs (tncRNAs): Hidden regulation of plants' transcriptional regulatory circuits.
Zahra et al (2021) Computational and Structural Biotechnology Journal, 19: 5278-5291