PPepDB-ID	"Peptide Name"	PMID	"Plant source"	"Plant Family"	"Peptide Family"	"Peptide function"	"Peptide function description"	"Activity against"	Sequence	"Sequence Length"	Validation	"Avg. Molecular wt(Da)"	"Monoisotopic molecular wt(Da)"	pI	Method	"Additional information"
PPepDB_5445	SCP19_ARATH	--NA--	"Arabidopsis thaliana"	Brassicaceae	--NA--	Signaling-peptide	"Acyltransferase; Carboxypeptidase; Complete proteome; Glycoprotein; Hydrolase; Protease; Secreted; Signal; Transferase; Zymogen | Serine carboxypeptidase-like 19 precursor (EC 3.4.16.-),(Sinapoylglucose--choline O-sinapoyltransferase) (EC 2.3.1.91) (SCT),(Protein SINAPOYLGLUCOSE ACCUMULATOR 2) [Contains: Serine,carboxypeptidase-like 19 chain A; Serine carboxypeptidase-like 19,chain B].Secreted (Potential)."	--NA--	EYNGTVPPLELTSFSWTKVANILYLEAPAGSGYSYAKTRRAFESSDTKQMFNVDPSNARCSNNLQAYDHCMSEIYSEHILLRNCKVDYVLADTPNIRTDRHQIDQFLRSWFVKHPEFISNPFYVGGDSYSGKIVPGAVQQISLGNEKGLTIRALNYSIVDDWRPWMMSSNQVAGYTRTYANKMTFATIKGGGHTAEYTPDMRNLSFIVLFLLTLFFIHHLVDASLLVKSLPGFEGPLPFELETGYVSIGENRCNSQNIPYTFEIFNAVPYHVNNSLKGFRSLIYSGDHDSMVPFSSTQAWPLINIQGYVLGNPVTDKNIETNYRVPFAHGMGLISDELFESLERSCGGKFQCSLMFRRWIDGEPLRRVMKEFSVNDSSSLPPPSCFTYRYFLSAFWANDENVRRALGVKKEVGKWSGDVELFYYFVKSERNPENDPLMIWLTGGPGCSSICGLLFANGPLAFKGD	465	"Experimental evidence at protein level"	52578.6	52544.9	6.1	--NA--	"FUNCTION:Involved in plants secondary metabolism. Functions as acyltransferase to form the sinapate ester sinapoylcholine also known as sinapine. May rather catalyze a transesterification reaction rather than a hydrolysis. May also have carboxypeptidase activity.CATALYTIC ACTIVITY:1-O-sinapoyl-beta-D-glucose choline = D- glucose sinapoylcholine.ENZYME REGULATION:Slightly inhibited by phenylmethylsulphonylfluoride (PMSF).BIOPHYSICOCHEMICAL PROPERTIES:Kinetic parameters: KM=0.16 mM for sinapoylglucose; KM=0.41 mM for feruloylglucose; KM=0.1 mM for caffeoylglucose; KM=1.9 mM for p-coumaroylglucose; KM=3.2 mM for choline; KM=19 mM for N,N-dimethylethanolamine; KM=245 mM for 2-metylaminoethanol; KM=25 mM for neopentyl alcohol; KM=105 mM for Tris; Vmax=2.8 nmol/sec/mg enzyme with sinapoylglucose as substrate; Vmax=1.8 nmol/sec/mg enzyme with feruloylglucose as substrate; Vmax=0.75 nmol/sec/mg enzyme with caffeoylglucose as substrate; Vmax=3.3 nmol/sec/mg enzyme with p-coumaroylglucose as substrate; Vmax=2.5 nmol/sec/mg enzyme with choline as substrate; Vmax=1.7 nmol/sec/mg enzyme with N,N-dimethylethanolamine as substrate; Vmax=1.2 nmol/sec/mg enzyme with 2-metylaminoethanol as substrate; Vmax=0.45 nmol/sec/mg enzyme with neopentyl alcohol as substrate; Vmax=0.32 nmol/sec/mg enzyme with Tris as substrate; Note=Measured in vitro at pH 7.0 and 30 degrees Celsius;SUBUNIT:Heterodimer (Potential).TISSUE SPECIFICITY:Expressed in roots and flowers, and at lower levels in young leaves and seedlings.PTM:N-glycosylated.MISCELLANEOUS:Plants lacking SCPL19 accumulate sinapoylglucose and contain low levels of sinapoylcholine and increased levels of choline.SIMILARITY:Belongs to the peptidase S10 family.SEQUENCE CAUTION:Sequence=BAB09519.1; Type=Erroneous gene model prediction; Sequence=CAB89366.1; Type=Erroneous gene model prediction; "