PPepDB-ID	"Peptide Name"	PMID	"Plant source"	"Plant Family"	"Peptide Family"	"Peptide function"	"Peptide function description"	"Activity against"	Sequence	"Sequence Length"	Validation	"Avg. Molecular wt(Da)"	"Monoisotopic molecular wt(Da)"	pI	Method	"Additional information"
PPepDB_5062	GLE_CHLRE	--NA--	"Chlamydomonas reinhardtii"	Chlamydomonadaceae	--NA--	Signaling-peptide	"Cell wall; Cell wall biogenesis/degradation; Direct protein sequencing; Glycoprotein; Hydrolase; Metal-binding; Metalloprotease; Protease; Secreted; Signal; Zinc; Zymogen | Autolysin precursor (EC 3.4.24.38) (Gametolysin) (Gamete lytic enzyme),(GLE).Note=Stored in the periplasm of gametes until its release. Secreted concurrently"	--NA--	DFSSCKLSGWSAPATLTPEKVTSDMLRGASAPTNNLANYYGACSYEKTLFDWWDLSKYCTASEQQAWERAAEAYAQAIVAQDPNSATGKKLQGILQWRERMSLATRRFGAAAALLVAACVLCTAPAWAQNETTGTGMVKTKSAFRWIRPPNPDNFLVLGPVPVPCIGGVTPPPRPPRPPRPPPRAGSTISSLSRRNDTYDNYGLEHAGRGTLEYGDATDVMGDFNKAGKGLLCPNAPNMYRIGWAKPINEPARPPPFRRPPPAQTPYVHKVEYTELQILCPQTIDSVTGYPMDDPRCNVPPGVAPFQNATGAWGNLTAANFTTDPWIRGLVIPAQGTRDDNMIVVNVGAQQAVVDICRISENGKELSCDDGIDNDCDGLQDNEDPDCQQRTGRVLLATSAELPTPTFRLKSLKSILKGSQKEIYAGKPIDLRTIVYIMRATVAAGEEALTIRNEFELLNGDVLNVTLEEVDTPENPSRRRLLSIIREERRNIYILPPGVKCSWSGYADVTCTSATCSAYVRGYSDTNAMQVIMHEAMHSERVFGFKSNLLDWGPNFQSRSNTWTSPFLAYNNGLGGGVRLVVQSTSDTSTRDGAMKATGAQAYYFSYRIKNTTAGGYDSGLTLDFHKKVLVHAYNGIQ	638	"Experimental evidence at protein level"	69832.86	69788.66	7.53	--NA--	"FUNCTION:Mediates digestion of the cell walls of the 2 mating type gametes during mating as a necessary prelude to cell fusion.This enzyme acts specifically on the framework proteins (inner wall) of the cell wall, cleaving several model peptides at specific sites.CATALYTIC ACTIVITY:Cleavage of the proline- and hydroxyproline- rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, gelatin and Leu-Trp-Met-|-Arg-Phe-Ala.COFACTOR:Binds 1 zinc ion per subunit (By similarity).INDUCTION:By the signal of flagellar agglutination between gametes of the opposite mating type.DOMAIN:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme.The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.PTM:Present in 2 forms: an inactive V-form in vegetative cells and an active and soluble G-form. The V-form enzyme may be converted to the G-form enzyme during gametic differentiation under nitrogen-starved conditions.SIMILARITY:Belongs to the peptidase M11 family."