PPepDB-ID	"Peptide Name"	PMID	"Plant source"	"Plant Family"	"Peptide Family"	"Peptide function"	"Peptide function description"	"Activity against"	Sequence	"Sequence Length"	Validation	"Avg. Molecular wt(Da)"	"Monoisotopic molecular wt(Da)"	pI	Method	"Additional information"
PPepDB_4273	AGAL_ORYSJ	--NA--	"Oryza sativa subsp. Japonica"	Poaceae	--NA--	Signaling-peptide	"3D-structure; Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase; Signal | Alpha-galactosidase precursor (EC 3.2.1.22) (Melibiase) (Alpha-D-,galactoside galactohydrolase)."	--NA--	AGSQTCSNKMPGSLDHEEQDVKTFASWGVDYLKYDNCNDAGRSVMERYTRARRRAFENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSMARASSSSSPPSPRLLLLLLVAVAATLLPEAAALGNFTAESRGARWRSRRMSNAMKTYGKNIFFSLCEWGKENPATWAGRMGNSWRTTGDIADNWGSMTSNNRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFAAQGQIQYVNIDDCWAEYSRDSQGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAKAPLLSASVAPHDCKMYVLTPN	417	"Experimental evidence at protein level"	45821.24	45792.28	8.14	--NA--	"FUNCTION:Hydrolyzes melibiose, raffinose and stachyose in the following decreasing order of reactivity: raffinose, melibiose, stachyose.CATALYTIC ACTIVITY:Hydrolysis of terminal, non-reducing alpha-D- galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.ENZYME REGULATION:1mM Hg(2) and Ag(2) decrease activity by 98% and 96% respectively. 1mM Para-chloromercuribenzoic acid (PCMB) completely inhibits enzymatic activity.BIOPHYSICOCHEMICAL PROPERTIES:pH dependence: Optimum pH is 5.0. Slowly inactivated above pH 8.0 and below pH 3; Temperature dependence: Optimum temperature is 45 degrees Celsius with p-nitrophenyl- alpha-D-galactopyranoside as substrate;SIMILARITY:Belongs to the glycosyl hydrolase 27 family.SEQUENCE CAUTION:Sequence=BAF26853.1; Type=Erroneous gene model prediction; "