PPepDB-ID	"Peptide Name"	PMID	"Plant source"	"Plant Family"	"Peptide Family"	"Peptide function"	"Peptide function description"	"Activity against"	Sequence	"Sequence Length"	Validation	"Avg. Molecular wt(Da)"	"Monoisotopic molecular wt(Da)"	pI	Method	"Additional information"
PPepDB_4220	SUBL_ARATH	--NA--	"Arabidopsis thaliana"	Brassicaceae	--NA--	Signaling-peptide	"Calcium; Cell wall; Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Protease; Secreted; Serine protease; Signal; Zymogen | Subtilisin-like protease precursor (EC 3.4.21.-) (Cucumisin-like,serine protease).Secreted, cell wall. Note=Intracellular spaces and cell wall."	--NA--	AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFALAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGMSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNPVAISWTVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPE	757	"Experimental evidence at protein level"	79415.37	79365.48	5.92	--NA--	"FUNCTION:Serine protease. Has a substrate preference for the hydrophobic residues Phe and Ala and the basic residue Asp in the P1 position, and for Asp, Leu or Ala in the P1' position.ENZYME REGULATION:Activated by calcium. Inhibited by the serine protease inhibitors 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), PMSF, di-isopropyl phosphofluoridate (DFP) and soybean trypsin inhibitor (SBTI). Not inhibited by benzamidine or iodoacetamide. Leupeptin and pepstatin A have a minor inhibitory action.BIOPHYSICOCHEMICAL PROPERTIES:pH dependence: Optimum pH is 5.0; Temperature dependence: Optimum temperature is 80 degrees Celsius. Thermostable;TISSUE SPECIFICITY:Expressed in immature siliques and at lower levels in stems and flowers.DEVELOPMENTAL STAGE:Highest levels of expression detected during silique development.MASS SPECTROMETRY:Mass=76102.8; Method=MALDI; Range=107-757; Source=PubMed:12413398;SIMILARITY:Belongs to the peptidase S8 family."